Association of MC4R Gene Polymorphisms with Growth and Body Composition Traits in Chicken

  • Li, Chun-Yu (College of Animal Science and Technology, Northeast Agricultural University) ;
  • Li, Hui (College of Animal Science and Technology, Northeast Agricultural University)
  • Received : 2005.08.18
  • Accepted : 2006.01.09
  • Published : 2006.06.01


Genetic and pharmacological studies in mice have demonstrated a complementary role for the melanocortin 4 receptor (MC4R) in the control of food intake, energy balance and body weight. This study was designed to investigate the associations of a MC4R gene polymorphism on chicken growth and body composition traits in broiler lines divergently selected for abdominal fat. A SNP (G54C) was found in CDS region of chicken MC4R gene. The analysis of the least squares and variance revealed a significant association between the G54C SNP and BW, CW and SL at 7 wk of age, and there were significant differences in different genotypes (p<0.05). The results from protein secondary structure prediction and tertiary structure prediction showed that it appeared a helix in $13^{th}$ amino acid and two strands at $14^{th}$ and $15^{th}$ amino acid in mutant protein, respectively. It maybe induce the change of the activity or function of MC4R gene in poultry.


Melanocortin 4 Receptor;SNP;Body Weight;Body Composition;Chicken


Supported by : National Natural Science Foundation


  1. Cone, R. D. 1999. The central melanocortin system and energy homeostasis. Trends Endocrinol. Metab. 10(6):211-215
  2. Deeb, N. and S. J. Lamont. 2002. Genetic architecture of growth and body composition in unique chicken population. J. Hered. 93:107-118
  3. Huszar, D., C. A. Lynch, V. Fairchild-Huntress, J. H. Dunmore, Q. Fang, L. R. Berkemeier, W. Gu, R. A. Kesterson, B. A. Boston, R. D. Cone, F. J. Smith, L. A. Campfield, P. Burn and F. Lee. 1997. Targeted disruption of the melanocortin-4 receptor results in obesity in mice. Cell. 88(1):131-141
  4. Hwa, J. J., L. Ghibaudi, J. Gao and E. M. Parker. 2001. Central melanocortin system modulates energy intake and expenditure of obese and lean Zucker rats. Am. J. Physiol. Regul. Integr. Comp. Physiol. 281:444-451
  5. Ikeobi, C. O. N., J. A. Woolliams, D. R. Morrice, A. Law, D. Windsor, D. W. Burt and P. M. Hocking. 2002. Quantitative trait loci affecting fatness in the chicken. Anim. Genet. 33:428- 435
  6. Kim, K. S., N. Larsen, T. Short, G. Plastow and M. F. Rothschild. 2000. A missense variant of the porcine melanocortin-4 receptor (MC4R) gene is associated with fatness, growth and feed intake traits. Mamm genome. 11:131-135
  7. King, P. J., P. S. Widdowson, H. Doods and G. Williams. 2000. Regulation of neuropeptide Y release from hypothalamic slices by melanocortin-4 agonists and leptin. Peptides. 21(1):45-48
  8. Koegler, F. H., K. L.Grove, A. Schiffmacher, M. S. Smith and J. L. Cameron. 2001. Central melanocortin receptors mediate changes in food intake in the Rhesus Macaque. Endocri. 6(142):2586-2592
  9. MacNeil, D. J., A. D. Howard, X. Guan, T. M. Fong, R. P. Nargund, M. A. Bednarek, M. T. Goulet, D. H. Weinberg, A. M. Strack, D. J. Marsh, H. Y. Chen, C. P. Shen, A. S. Chen, C. I. Rosenblum, T. MacNeil, M. Tota, E. D. MacIntyre and L. H. Van der Ploeg. 2002. The role of melanocortins in body weight regulation: opportunities for the treatment of obesity. Eur. J. Pharmacol. 450:93-109
  10. Mizuno, T. M. and C. V. Mobbs. 1999. Hypothalamic agoutirelated protein messenger ribonucleic acid is inhibited by leptin and stimulated by fasting. Endocri. 140:814-817
  11. Rothschild, M. F. and M. Soller. 1997. Candidate gene analysis to detect genes controlling traits of economic importance in domestic livestock. Probe. 8:13-20
  12. Schwartz, M. W., S. C. Woods, D. J. Porte, R. J. Seeley and D. G. Baskin. 2000. Central nervous system control of food intake. Nat. 404:661-671
  13. Strader, A. D., H. B. SchiÖth and J. D. Buntin. 2003. The role of the melanocortin system and the melanocortin-4 receptor in ring dove (Streptopelia risoria) feeding behavior. Brain Res. 960:112-121
  14. Vergoni, V. A. and A. Bertolini. 2000. Role of melanocortins in the central control of feeding. Eur. J. Pharmacol. 405:25-32
  15. Zhang, B. Z., M. G. Lei, C. Y. Deng, Y. H. Xiong, B. Zuo and F. E. Li. 2005. Association between PCR-RFLP polymorphism of the fifth intron in lipoprotein lipase gene and productive traits in pig resource family. Asian-Aust. J. Anim. Sci. 18(4):458- 462
  16. Li, H., N. Deeb, H. Zhou, A. D. Mitchell, C. M. Ashwell and S. J. Lamont. 2003. Chicken quantitative trait loci for growth and body composition associated with transforming growth factor- ${\beta}$ genes. Poult. Sci. 82:347-356
  17. Dumont, L. M., C. S. Wu, M. A. Tatnell, J. Cornish, K. G. Mountjoy. 2005. Evidence for direct actions of melanocortin peptides on bone metabolism. Peptides. 26:1929-1935
  18. Hagan, M. M., P. A. Rushing, M. W. Schwartz, K. A. Yagaloff, P. Brun, S. C. Wodds and R. J. Seely. 1999. Role of the CNS melanocortin system in the response to overfeeding. J. Neurosci. 19(6):2362-2367
  19. Boldman, K. and D. V. Vleck. 2002. MTDFREML manual, United States Department of Agriculture, USA
  20. Julian, R. J. 1998. Rapid growth problems: ascites and skeletal deformities in broilers. Poult. Sci. 77:1773-1780
  21. Vaisse, C., K. Clement, E. Durand, S. Hercberg, B. Guy-Grand and P. Froguel. 2000. Melanocortin-4 receptor mutations are a frequent and heterogeneous cars of morbid obesity. J. Clin. Inverst. 106(2):253-262
  22. Xu, D. Q., Y. Z. Xiong, M. Liu, J. Lan, X. F. Ling, C. Y. Deng and S. W. Jiang. 2005. Association analyses with carcass traits in the porcine KIAA1717 and HUMMLC2B genes. Asian-Aust. J. Anim. Sci. 18(11):1519-1541
  23. Robert, B. 2005. The complex interplay between fat and bone. Cellscience Reviews. 4:71-77
  24. Steppan, C. M., D. T. Crawford, K. L. Chidsey-Frink, H. Ke and A. G. Swick. 2000. Leptin is a potent stimulator of bone growth in ob/ob mice. Regul Pept. 92:73-78
  25. Chung, E. R. and W. T. Kim. 2005. Association of SNP Marker in IGF-I and MYF5 candidate genes with growth graits in korean cattle. Asian-Aust. J. Anim. Sci. 18(8):1061-1065
  26. Hinney, A., A. Schmidt, K. Nottebom, O. Heibult, W. Beckfried, M. Fichter, H. Remschmidt and J. Hebebrand. 1999. Several mutations in the melancortin-4 receptor gene including a nonsense and a frame shift mutaion associated with dominantly inherited obesity in humans. J. Clin. Endocrionl. Metab. 84(4):1483-1486
  27. Meng, H., J. G. Zhao, Z. H. Li and H. Li. 2005. Single nucleotide polymorphism on peroxisome proliferators- activated receptor genes associated with fatness traits in chicken. Asian-Aust. J. Anim. Sci. 18(9):1221-1225
  28. Vaisse, C., K. Clement, B. Guy-Grand and P. Froguel. 1998. A frame shift mutation in human MC4R is associated with a dominant form of obesity. Nat Genet. 20(2):113-114
  29. Baskin, D. G., T. M. Hahn and M. W. Schwartz. 1999. Leptin sensitive neurons in the hypothalamus. Horm. Metab. Res. 31:345-350
  30. Kask, A., L. Rago, J. Wikberg and H. B. Schioth. 1998. Evidence for involvement of the melanocortin 4 receptor in the effects of leptin on food intake and body weight. Eur. J. Pharmacol. 360 (1):15-19
  31. Yang, Y. K., D. A. Thompson, C. J. Dickinson, J. Wiken, G. S. Barsh, S. B. Kent and I. Gantz. 1999. Characterization of agouti-related protein binding to melanocortin receptors. Mol. Endocrinol. 13:148-155
  32. Ho, G. and R. G. Mackenzie. 1999. Functional characterization of mutations in melanocortin-4 receptor associated with human obesity. J. Biol. Chem. 274(50):35816-35822
  33. Roger, A. H. and W. H. Gispen. 1997. Brain melanocortin receptors: from cloning to function. Reptides. 8:1279-1287
  34. Kim, T. H., B. H. Choi, H. K. Lee, H. S. Park, H. Y. Lee, D. H. Yoon, J. W. Lee, G. J. Jeon, I. C. Cheong, S. J. Oh and J. Y. Han. 2005. Identification of quantitative traits loci (QTL) affecting growth traits in pigs. Asian-Aust. J. Anim. Sci. 18(11):1524-1528
  35. Butler, A. A., R. A. Kesterson, K. Khong, M. J. Cullen, M. A. Pelleymounter, J. Dekoning, M. Baetscher and R. D. Cone. 2000. A unique metabolic syndrome causes obesity in the melanocortin-3 receptor-deficient mouse. Endocri. 141(9):3518-3521
  36. Schwartz, M. W., R. J. Seeley, L. A. Campfield, P. Burn and D. G. Baskin. 1996. Identification of targets of leptin action in rat hypothalamus. J. Clin. Invest. 98:1101-1106
  37. Jiang, Y. L., N. Li, X. Z. Fan, L. R. Xiao, R. L. Xiang, X. X. Hu, L. X. Du and C. X. Wu. 2002b. Associations of $T^{0}CA$ mutation in the promoter region of myostatin gene with birth weight in Yorkshire pigs. Asian-Aust. J. Anim. Sci. 15(11):1543-1545
  38. Takeuchi, S. and S. Takahashi. 1999. A possible involvement of melanocortin 3 receptor in the regulation of adrenal gland function in the chicken. Biochim. Biophys. Acta. 1448(3):512- 518

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