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Efficacy of Glucomannan-containing Yeast Product (Mycosorb®) and Hydrated Sodium Calcium Aluminosilicate in Preventing the Individual and Combined Toxicity of Aflatoxin and T-2 Toxin in Commercial Broilers

  • Girish, C.K. (College of Veterinary Medicine, University of Agricultural Sciences) ;
  • Devegowda, G. (College of Veterinary Medicine, University of Agricultural Sciences)
  • Received : 2005.02.22
  • Accepted : 2005.08.22
  • Published : 2006.06.01

Abstract

A feeding trial was conducted on commercial broilers for a period of 35 days to determine the individual and combined effects of aflatoxin (AF) and T-2 toxin (T-2) on performance, organ weights and immune status. The efficacy of dietary glucomannan-containing yeast product (GYP) ($Mycosorb^{(R)}$) and hydrated sodium calcium aluminosilicate (HSCAS) in preventing the adverse effects of aflatoxin and T-2 toxin was also evaluated. Twelve dietary treatments ($4{\times}3$ factorial) comprising two dietary levels each of AF (0 and 2 mg/kg), T-2 toxin (0 and 1 mg/kg), GYP (0 and 1 kg/ton) and HSCAS (0 and 10 kg/ton) were tested on 720 commercial broiler chickens divided at random into 36 replicates of 20 chicks each (10 males and 10 females). Weight gain and feed intake were recorded weekly. Organ morphology and antibody titers for Newcastle disease (ND) and infectious bursal disease (IBD) were measured on the $35^{th}$ day. AF and T-2 toxin individually decreased weight gain and increased feed conversion ratio (FCR) (p<0.05). AF alone (p<0.05) increased weights of liver, kidney, gizzard and spleen and reduced thymus and bursal weights. T-2 toxin (p<0.05) increased liver and gizzard weights and decreased thymus weight. Both AF and T-2 toxin when fed individually affected ND and IBD titers in a significant manner. Significant interactions between AF and T-2 toxin were observed for their additive effects on weight gain, FCR, organ weights and antibody titers. Addition of GYP (p<0.05) improved weight gain, feed conversion efficiency and restored the organ weights. Antibody titers against ND and IBD were significantly improved with the supplementation of GYP. Supplementation of HSCAS (p<0.05) resulted in improvement in weight gain and restored organ weights in the groups fed AF alone, but not in T-2 toxin fed groups. HSCAS inclusion did not influence FCR in toxin fed groups. Addition of HSCAS (p<0.05) improved the antibody titers against ND and IBD only in AF fed groups. Thus, the results indicate that addition of GYP is effective in averting the individual and combined toxicity of aflatoxin and T-2 toxin in commercial broilers, while HSCAS is effective only against aflatoxin.

Keywords

Glucomannan-containing Yeast Product;HSCAS;Aflatoxin;T-2 Toxin;Broiler Chickens

References

  1. AOAC. 1995. Official methods analysis. 16th edn. Association of official analytical chemists, Washington, DC
  2. Bureau of Indian Standards. 1992. Nutrient requirements of Poultry. Bureau of Indian standards, Manak Bhavan, New Delhi
  3. Chestnut, A. B., P. D. Anderson, M. A. Cochran, H. A. Fribourg and K. D. Twinn. 1992. Effects of Hydrated Sodium Calcium Aluminosilicate on Fescue Toxicosis and Mineral Absorption. J. Anim. Sci. 70:2838-2846 https://doi.org/10.2527/1992.7092838x
  4. Devegowda, G., B. I. R. Aravind, M. G. Morton and K. Rajendra. 1995. A biotechnological approach to counteract aflatoxicosis in broiler chickens and ducklings by the use of Saccharomyces cerevisiae. In: Proc. Feed Ingredients Asia '95, Singapore. pp. 161-171
  5. Huff, W. E., L. F. Kubena, R. B. Harvey and T. D. Phillips. 1992. Efficacy of hydrated sodium calcium aluminosilicate to reduce the individual and combined toxicity of aflatoxin and ochratoxin A. Poult. Sci. 71:64-69 https://doi.org/10.3382/ps.0710064
  6. Jindal, N., S. K. Mahipal and N. K. Mahajan. 1993. The effect of hydrated sodium calcium aluminosilicate on prevention of aflatoxicosis in broilers. Indian J. Anim. Sci. 63:649-652
  7. Kubena, L. F., R. B. Harvey, W. E. Huff, D. E. Corrier, T. D. Phillips and G. E. Rottinghaus. 1989. Influence of ochratoxin A and T-2 toxin singly and in combination on broiler chickens. Poult. Sci. 68:867-972 https://doi.org/10.3382/ps.0680867
  8. Kubena, L. F., R. B. Harvey, W. E. Huff, D. E. Corrier, T. D. Phillips and G. E. Rottinghaus. 1990. Efficacy of hydrated sodium calcium aluminosilicate to reduce the toxicity of aflatoxin and T-2 toxin. Poult. Sci. 69:1078-1086 https://doi.org/10.3382/ps.0691078
  9. Kubena, L. F., R. B. Harvey, S. A. Buckley, T. S. Edrington and G. E. Rottinghaus. 1997a. Individual and combined effects of moniliformin present in Fusarium fujikuroi culture material and aflatoxin in broiler chicks. Poult. Sci. 76:265-270 https://doi.org/10.1093/ps/76.2.265
  10. Rukmini, C. and R. V. Bhatt. 1978. Occurrence of T-2 toxin in Fusarium infested sorghum from India. J. Agric. Food Chem. 26:647-649 https://doi.org/10.1021/jf60217a013
  11. Sengstag, C. 1997. The molecular mechanism of aflatoxin B1- induced liver cancer: is mitotic recombination involved. Molecular Carcinogenesis, 19:147-152 https://doi.org/10.1002/(SICI)1098-2744(199707)19:3<147::AID-MC1>3.0.CO;2-B
  12. Swamy, H. V. L. N. and G. Devegowda. 1998. Ability of mycosorb to counteract aflatoxicosis in commercial broilers. Indian J. Poult. Sci. 33:273-278
  13. Ueno, Y. 1977. Mode of action of trichothecenes. Pure Appl. Chem., 49:1737-3745 https://doi.org/10.1351/pac197749111737
  14. Dvorska, J. E. and P. F. Surai. 2001. Effect of T-2 Toxin, zeolite and mycosorb on antioxidant systems of growing quail. Asian-Aust. J. Anim. Sci. 14(12):1752-1757 https://doi.org/10.5713/ajas.2001.1752
  15. Kubena, L. F., R. B. Harvey, R. H. Bailey, S. A. Buckley and G. E. Rottinghaus. 1998. Effect of a hydrated sodium calcium aluminosilicate (T-bind) on mycotoxicosis in young broiler chickens. Poult. Sci. 77 (10):1502-1504 https://doi.org/10.1093/ps/77.10.1502
  16. Moshtaghian, J., C. M. Parsons, R. W. Leeper, P. C. Harrison and K. W. Koelkebeck. 1991. Effect of sodium aluminosilicate on phosphorus utilization by chicks and laying hens. Poult. Sci. 70:955-962 https://doi.org/10.3382/ps.0700955
  17. Nelson, T. S., Z. B. Johnson, L. K. Kirby and J. N. Beasley. 1982. Digestion of dry matter and amino acids and energy utilization by chicks fed molded corn containing mycotoxins. Poult. Sci. 61:534-585
  18. Hoerr, F. J., W. W. Carlton and B. Yagen. 1981. Mycotoxicosis caused by a single dose of T-2 toxin or diacetoxyscirpenol in broiler chickens. Vet. Path. 18:652-664 https://doi.org/10.1177/030098588101800510
  19. Huff, W. E., R. B. Harvey, L. F. Kubena and G. E. Rottinghaus. 1988. Toxic synergism between aflatoxin and T-2 toxin in broiler chickens. Poult. Sci. 67:1418-1423 https://doi.org/10.3382/ps.0671418
  20. Lyons, T. P. 1994. In: Biotechnology in the Feed industry (Ed. T. P. Lyons and K. A. Jacques). Proceedings of the Alltech's 10th Annual symposium, Kentucky, pp. 1-48
  21. Burmeister, H. R. 1971. T-2 Toxin production by Fusarium tricinctum on solid substrate. Appli. Micro. 21:739-742
  22. Hoerr, F. J., W. W. Carlton, B. Yagen and A. Z. Joffe. 1982. Mycotoxicosis produced in broiler chickens by multiple doses of either T-2 toxin or diacetoxyscirpenol. Avian. Pathol. 11:269-383
  23. Devegowda, G., B. I. R. Arvind and M. G. Morton. 1996. Saccharomyces cerevisiae and mannanoligosaccharide to counteract aflatoxicosis in broilers. In: Proc. Aust. Poult. Sci. Symp, Sydney, Australia. 8:103-106
  24. Santurio, J. M., C. A. Mallmann, A. P. Rosa, G. Appel, A. Heer, S. Dageforde and M. Bottcher. 1999. Effect of sodium bentonite on the performance and blood variables of broiler chickens intoxicated with aflatoxin. Br. Poult. Sci. 40:115-119 https://doi.org/10.1080/00071669987935
  25. Arvind, K. L., V. S. Patil, G. Devegowda, B. Umakantha and S. P. Ganpule. 2003. Efficacy of esterified glucomannan to counteract Mycotoxicosis in naturally contaminated feed on performance, serum biochemical and hematological parameters in broilers. Poult. Sci. 82:571-576 https://doi.org/10.1093/ps/82.4.571
  26. Rizzi, L., A. Zaghini and P. Roncoda. 1998. Aflatoxin $B_1$ oral administrations to laying hens: Efficacy of modified mannanoligosaccharide (Mycosorb) to prevent mycotoxicosis. Enclosure Code Myco 1.5 in Biotechnology in the Feed Industry (Ed. T. P. Lyons and K. A. Jacques). Nottingham University Press, Loughborough, Leics, UK
  27. Whitlow, L. W., D. E. Diaz, B. A. Hopkins and W. M. Hagler, Jr., 2000. Mycotoxins and milk safety: the potential to block transfer to milk. Pages 391-408 In: Biotechnology in the feed industry (Ed. T. P. Lyons and K. A. Jacques), Nottingham University Press, Nottingham, UK
  28. Atroshi, F., A. Rizzo, T. Westermarck and T. Ali-vehmas. 2002. Antioxidant nutrients and mycotoxins. Toxicology 180:151- 167 https://doi.org/10.1016/S0300-483X(02)00388-8
  29. Raju, M. V. L. N. and G. Devegowda. 2000. Influence of modified glucomannan on performance and organ morphology, serum biochemistry and hematology in broilers exposed to individual and combined mycotoxicosis (Aflatoxin, Ochratoxin and T-2 toxin). Br. Poult. Sci. 41:640-650 https://doi.org/10.1080/713654986
  30. Smith, T. K. 1984. Spent canola oil bleaching clays: potential for treatment of T-2 toxicosis in rats and short-term inclusion in diets for immature swine. Can. J. Anim. Sci. 64:725-732 https://doi.org/10.4141/cjas84-080
  31. Harvey, R. B., L. F. Kubena, M. H. Elissalde and T. D. Philips. 1993. Efficacy of zeolite ore compounds on the toxicity of aflatoxin to growing broiler chickens. Avian diseases. 37:67-73 https://doi.org/10.2307/1591459
  32. Mahesh, B. K. and G. Devegowda. 1996. Ability of Aflatoxin B1 binders to bind aflatoxin B1 in liquid media-an in vitro study. Proc. 20th World's Poultry Congress, New Delhi, 4:295-296
  33. Afzal, M. and Z. Saleem. 2004. Effects of addition of a mycotoxin detoxifier in poultry feed containing different levels of aflatoxins on the performance of broilers. Asian-Aust. J. Anim. Sci. 17(7):990-994 https://doi.org/10.5713/ajas.2004.990
  34. Devegowda, G., M. V. L. N. Raju, N. Afzali and H. V. L. N. Swamy. 1998b. Mycotoxin picture worldwide: Novel solutions for their counteraction. In: Biotechnology in the Feed Industry: Proceedings of Alltech's 14th Annual Symposium (Ed. T. P. Lyons and K. A. Jacques). Nottingham University Press, Nottingham. pp. 241-255
  35. Phillips, T. D., B. A. Clement, L. F. Kubena and R. B. Harvey. 1990b. Detection and detoxification of aflatoxins: Prevention of aflatoxicosis and aflatoxin residues with hydrated sodium calcium aluminosilicate. Vet. Human Toxicol. 32:15-19
  36. Romer, T. R., T. M. Boling and J. L. Macdonald. 1978. Gas-liquid chromatographic determination of T-2 toxin and diacetoxyscirpenol in corn and mixed feeds. J. Assoc. Off. Anal. Chem. 6:801-807
  37. Barmase, B. S., G. Devegowda and U. Devurkar. 1990. Reversal of aflatoxicosis through dietary adsorbents in broiler chickens. Proc. 13th Annual Conf. Symp. Indian Poult. Sci. Assn. Bombay:20-22
  38. Savage, T. F., P. F. Cotter and E. I. Zakrzewska. 1996. The effect of feeding Mannanoligosaccharide on immunoglobulins, plasma IgG and bile IgA of Worldstad MW male turkeys. Poult. Sci. 75(s1):143
  39. Devegowda, G. and T. N. K. Murthy. 2005. Mycotoxins: Their adverse effects in poultry and some practical solutions. In: The Mycotoxin Blue Book (Ed. D. E. Diaz). Nottingham University Press, Nottingham, UK. pp. 25-56
  40. Johri, T. S., J. Verma and A. K. Srinivastav. 1996. The individual and combined effects of aflatoxin and ochratoxin on protein quality and metabolizability of energy in broilers. Proceedings of 20th World's Poultry Congress, New Delhi, 4:275
  41. Murthy, T. N. K. and G. Devegowda. 2004. Efficacy of modified glucomannan (Mycosorb) to adsorb aflatoxin B1 in gut conditions of broiler chickens. In Proc.: 22nd World's Poultry Congress, Istanbul, Turkey. pp. 471
  42. SAS Institute, 1996, SAS$^{\circledR}$ User's Guide: Statistics. SAS Institute Inc., Cary, NC, USA
  43. Dwivedi, P. and R. B. Burns. 1984. Pathology of ochratoxicosis A in young broiler chicks. Res. Vet. Sci. 36:92-103
  44. Devegowda, G., M. V. L. N. Raju, N. Afzali and H. V. L. N. Swamy. 1998a. Mycotoxin picture worldwide: Novel solutions for their counteraction. Feed Compounder, 18(6):22-27
  45. Ledoux, D. R., G. E. Rottinghaus, A. J. Bermudez and M. Alomo Debolt. 1999. Efficacy of a hydrated sodium calcium aluminosilicate to ameliorate the toxic effects of aflatoxin in broiler chicks. Poult. Sci. 78:204-210 https://doi.org/10.1093/ps/78.2.204
  46. Raju, M. V. L. N. and G. Devegowda. 2002. Esterifed glucomannan in broiler chicken diets contaminated with aflatoxin, ochratoxin and T-2 toxin: Evaluation of its binding ability (in vitro) and Efficacy as immunomodulator. Asian-Aust. J. Anim. Sci. 15:1051-1056 https://doi.org/10.5713/ajas.2002.1051
  47. Phillips, T. D., A. B. Sarr, B. A. Clement, L. F. Kubena and R. B. Harvey. 1990a. Prevention of aflatoxicosis in farm animals via selective chemisorption of aflatoxin. Pages 223-237 in: Pennington Center Nutrition Series. Vol. 1, Mycotoxins, Cancer and Health (Ed. G. A. Bray and D. H. Ryan). Louisiana State University Press, Baton Rouge, LA
  48. Shotwell, O. L., C. W. Hesseltine, R. D. Stubblefield and W. G. Sorenson. 1966. Production of aflatoxin on rice. Appl. Microbiol. 14:425-428
  49. Swamy, H. V. L. N., T. K. Smith, P. F. Cotter, H. J. Boermans and A. E. Seftons. 2002. Effects of feeding blends of grains naturally contaminated with Fusarium mycotoxins on prodution and metabolism in Broilers. Poult. Sci. 81:966-975 https://doi.org/10.1093/ps/81.7.966
  50. Chandrasekaran, D. 1996. Survey on the presence of T-2 and ochratoxin in feed/ingredients in Namakkal area. In: Proceedings of the 20th World's poultry congress, New Delhi, 4:268
  51. Edrington, T. S., L. F. Kubena, R. B. Harvey and G. E. Rottinghaus. 1997. Influence of super activated charcoal on the toxic effects of aflatoxin or T-2 toxins in growing broilers. Poult. Sci. 76:1205-1211 https://doi.org/10.1093/ps/76.9.1205
  52. Kubena, L. F., T. S. Edrington, R. B. Harvey, S. A. Buckley, T. D. Phillips, G. E. Rottinghaus and H. H. Caspers. 1997b. Individual and combined effects of fumonisin B1 present in Fusarium moniliforme culture material and T-2 toxin or deoxynivalenol in broiler chicks. Poult. Sci. 76:1239-1247 https://doi.org/10.1093/ps/76.9.1239
  53. Bailey, R. H., L. F. Kubena, R. B. Harvey, S. A. Buckley and G. E. Rottinghaus. 1998. Efficacy of various inorganic sorbents to reduce the toxicity of aflatoxin and T-2 toxin in broiler chickens. Poult. Sci. 77:1623-1630 https://doi.org/10.1093/ps/77.11.1623
  54. Yu, F. L. 1981. Studies on the mechanism of aflatoxin $B_1$ inhibition of rat liver nucleolar RNA synthesis. J. Biol. Chem. 256:3292-3297

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