Silk fibroin/hyaluronic acid blend sponge accelerates the wound healing in full-thickness skin injury model of rat

전층피부창상에서 실크피브로인과 하이알론산 혼합 스폰지의 창상치유효과

  • Kang, Seuk-Yun (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Roh, Dae-Hyun (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Kim, Hyun-Woo (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Yoon, Seo-Yeon (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Kwon, Young-Bae (Institute for Medical Science, Chonbuk National University Medical School) ;
  • Kweon, HaeYong (National Institute of Agricultural Science and Technology) ;
  • Lee, Kwang-Gill (National Institute of Agricultural Science and Technology) ;
  • Park, Young-Hwan (College of Agriculture and Life Sciences, Seoul National University) ;
  • Lee, Jang-Hern (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
  • 강석윤 (서울대학교 수의과대학) ;
  • 노대현 (서울대학교 수의과대학) ;
  • 김현우 (서울대학교 수의과대학) ;
  • 윤서연 (서울대학교 수의과대학) ;
  • 권영배 (전북대학교 의과대학) ;
  • 권해용 (국립농업과학진흥청 잠사연구소) ;
  • 이광길 (국립농업과학진흥청 잠사연구소) ;
  • 박영환 (서울대학교 농업생명과학대학) ;
  • 이장헌 (서울대학교 수의과대학)
  • Accepted : 2006.10.10
  • Published : 2006.12.30

Abstract

The primary goal of the wound healing is rapid wound closure. Recent advances in cellular and molecular biology have greatly expanded our understanding of the biologic processes involved in wound repair and tissue regeneration. This study was conducted to develop a new sponge type of biomaterial to be used for either wound dressing or scaffold for tissue engineering. We designed to make a comparative study of the wound healing effect of silk fibroin/hyaluronic acid (SF/HA) blend sponge in full-thickness dermal injury model of rat. Two full-thickness excisions were made on the back of the experimental animals. The excised wound was covered with either the silk fibroin (SF), hyaluronic acid (HA) or SF/HA (7 : 3 or 5 : 5 ratio) blend sponge. On the postoperative days of 3, 7, 10 and 14, the wound area was calculated by image analysis software. Simultaneously, the tissues were stained with Hematoxylin-Eosin and Masson's trichrome methods to measure the area of regenerated epithelium and collagen deposition. In addition, we evaluated the degree of the epithelial cell proliferation using immunohistochemistry for proliferating cell nuclear antigen (PCNA). We found that the half healing time ($HT_{50}$) of SF/HA blend sponge treated groups were significantly decreased as compared with either those of SF or HA treatment group. Furthermore, SF/HA blend sponges significantly increased the size of epithelialization and collagen deposition as well as the number of PCNA positive cells on epidermal basement membrane as compared with those of control treatment. Especially, the 5 : 5 ratio group of SF/HA among all treatment groups was most effective on wound healing rate and histological studies. These results suggest that SF/HA blend sponges could accelerate the wound healing process through the increase of epithelialization, collagen deposition and basal cell proliferation in full thickness skin injury.

Acknowledgement

Supported by : 한국과학재단, 전북대학교

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