Establishment of ethanol-pretreating animal model to study Helicobacter pylori infection

Helicobacter pylori의 in vivo 연구를 위한 ethanol-pretreating animal model의 개발

  • Lee, Jin-Uk (College of Medicine, Seoul National University) ;
  • Kim, Seung-Hee (College of Medicine, Seoul National University) ;
  • Park, Tan-Woo (College of Life Science and Natural Resources, Wonkwang University) ;
  • Kim, Okjin (College of Life Science and Natural Resources, Wonkwang University)
  • 이진욱 (서울대학교 의과대학 실험동물학교실) ;
  • 김승희 (서울대학교 의과대학 실험동물학교실) ;
  • 박탄우 (원광대학교 생명자원과학대학 동물질병연구실) ;
  • 김옥진 (원광대학교 생명자원과학대학 동물질병연구실)
  • Accepted : 2006.09.19
  • Published : 2006.12.30

Abstract

A stable and reliable Helicobacter pylori (H. pylori) infection animal model would be necessary for evaluating vaccine efficacy and helpful for understanding the pathological mechanism of the organism. The aim of the present study is to investigate the effect of ethanol treatment prior to H. pylori inoculation on associated gastric mucosal injury and to establish ethanol-pretreating animal model to study H. pylori infection. Male Mongolian gerbils were used for the study. H. pylori was orally inoculated after 12 h fasting. 3 h prior to H. pylori inoculation, a group of gerbils was orally treated with absolute ethanol, 60% and 40% ethanol respectively. Another group of animals was treated either with H. pylori culture media alone or with different concentrations of ethanol plus culture media. Gerbils were killed 4 or 8 weeks after H. pylori inoculation. The colonization of H. pylori was confirmed by both histological examination and rapid urease test. Mucosal damage was evaluated grossly and histologically according to the criteria. The colonization of H. pylori and pathological changes in gastric mucosa of the animals were also observed. Although no significant change to the gastric mucose was observed in the animals treated either with H. pylori culture media alone or with different concentrations of ethanol plus culture media, persistent H. pylori infection was seen in the mucosa and mucosal leucocyte infiltration and severe epithelial damage was observed in the Helicobacter and ethanol + Helicobacter groups after 4 weeks. The gross and histological scores were higher in the ethanol + Helicobacter than in the Helicobacter alone group. As the results, ethanol-pretreatment with 60% concentration induced severe pathogenic changes by H. pylori infection in 5 weeks-old Mongolian gerbils. These results suggested that ethanol-pretreatment before H. pylori inoculation could increase the severity of gastric mucosal inflammation and enhance the colonization of H. pylori. The established ethanol-pretreating animal model would contribute to screen new drugs against H. pylori and be used as an useful tool for various animal experiments with H. pylori strains.

Acknowledgement

Supported by : 한국학술진흥재단

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