Determination of Fructooligosaccharides and Raffinose in Infant Formula by High Performance Liquid Chromatography with Evaporative Light Scattering Detector

HPLC-ELSC를 이용한 조제분유 중 fructooligosaccharides 및 raffinose 분석

  • Shin, Man-Sub (National Veterinary Research and Quarantine Service) ;
  • Park, Jae-Woo (National Veterinary Research and Quarantine Service) ;
  • Cho, Mi-Ran (National Veterinary Research and Quarantine Service) ;
  • Song, Sung-Ok (National Veterinary Research and Quarantine Service) ;
  • Kim, Chun-Sun (National Veterinary Research and Quarantine Service) ;
  • Choi, Chun-Bae (National Veterinary Research and Quarantine Service) ;
  • Lee, Seoung-Won (National Veterinary Research and Quarantine Service) ;
  • Lee, Ki-Woong (Research and Development Center, Namyang Dairy Products Corporation) ;
  • Chang, Chi-Hoon (Research and Development Center, Namyang Dairy Products Corporation) ;
  • Kwak, Byung-Man (Research and Development Center, Namyang Dairy Products Corporation)
  • Published : 2006.12.31

Abstract

A method was developed for the determination of fructooligosaccharides and raffinose contents in infant formula. The samples were extracted and analyzed by liquid chromatography equipped with carbohydrate column and evaporative light scattering detector. The mobile phase used for the gradient mode was water-acetonitrile, at a flow rate of 1.0mL/min. The method showed a mean recovery of 95-99%, the relative standard deviation obtained in the precision study was 0.774-8.982%, the quantification and detection limits were 25-50mg/L.

Keywords

fructooligosaccharide;raffinose;infant formula;HPLC;evaporative light scattering detector

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