Optimization of Conditions for the Double Layer Microencapsulation of Lactic Acid Bacteria

유산균 함유 이중층 미세캡슐화를 위한 조건 최적화

  • Published : 2006.12.31


In this study, we sought to produce a double layer microcapsule containing Lactobacillus sp. as the core material. The conditions for this microencapsulation process were optimized for the formation of a microcapsule with high storage stability. The effects of the ratio of[core material] to [wall material], the type and concentration of emulsifier used, the stirring rate(dispersibility) and the temperature of the dispersion fluid on the microencapsulation yield were studied. The optimal concentration and type of emulsifier required in order to allow for the stable formation of a W/O type emulsion (a primary process in double layer microencapsulation) were 1.00% (w/w) and polyglycerol polyricinileate (PGPR, HLB 0.6). However, the optimal concentration and type of emulsifier required to construct a W/O/W type emulsion (a secondary process in double layer microencapsulation), were 0.65% (w/w) and polyoxyethylene sorbitan monolaurate (PSML, HLB 16.7). Finally, we obtained a maximum yield of microencapsulation with a dispersion fluid stirring rate of 270rpm and a dispersion fluid temperature of 10$^{\circ}C$ after spraying a W/O/W type emulsion into the dispersion fluid.


emulsion;double layer microencapsulation;Lactobacillus;emulsifer


  1. Cho YE, Shin DS, Park JY. Microencapsulation technology in food industry. Food Sci. Ind. 30: 98-111 (1997)
  2. Chang PS. Determination of emulsion stability index in W/O emulsion. Anal. Sci. Technol. 7: 233-236 (1994)
  3. Chang PS. Microencapsulation and oxidative stability of docosahexaenoic acid. Am. Chem. Soc. Symp. 674: 264-274 (1997)
  4. Bakan JA. Microencapsulation of foods and related products. Food Technol. 27: 34-40 (1985)
  5. Deeth HC. Yoghurt and cultures products. Aus. J. Dairy Technol. 39: 111-113 (1984)
  6. Dziezak JD. Microencapsulation and encapsulated ingredient. Food Technol. 42: 136-151 (1988)
  7. Baik OR, Uy R, Byun SM. Hydrolysis of lactose in milk by microencapsulation of ${\beta}$-galactosidase. Korean. J. Food Sci. Technol. 12(1): 45-52 (1980)
  8. Morichi T. Preservation of lactic acid bacteria by freeze-drying. Jpn. Agric. Res. Quart. 8: 171-176 (1974)
  9. Chilvers GR, Gunning AP, Morris VJ. Coacervation of gelatin-XM6 mixture and their use in microencapsulation. Carbohydr. Polym. 8: 55-61 (1988) https://doi.org/10.1016/0144-8617(88)90036-7
  10. Deasy PB, Microencapsulation and Related Drug Processes. Marcel Dekker. Inc., New York, NY, USA. pp. 26-35 (1984)
  11. Jackson LS, Lee. K. Microencapsulated iron for food fortification. J. Food Sci. 56: 1047-1050 (1991) https://doi.org/10.1111/j.1365-2621.1991.tb14638.x
  12. Lee SC, Im CH, Lee Sc. Characteristics of spray dried polysaccharide for microencapsulation. Korean J. Food Sci. Technol. 29: 1322-1326 (1997)
  13. Kim CH, Lee KW, Kwak HS, Kang OJ. Studies on the microencapsulation of ${\omega}$-3 polyunsaturated fatty acid. Korean J. Food Sci. Technol. 28: 743-749 (1996)
  14. Arshady R. Microcapsules for food. J. Microencapsul. 10: 413-435 (1975) https://doi.org/10.3109/02652049309015320
  15. Magee EL, Olson NF, Lindsay RC. Microencapsulation of cheese ripening system: Production of diacetyl and aceton in cheese by encapsulated bacterial cell-free extract. J. Dairy Sci. 64: 616-621 (1981) https://doi.org/10.3168/jds.S0022-0302(81)82620-3
  16. Tharwat FT, Brian V. Emulsion stability. Vol. I, pp. 129-286. In: Encyclopedia of Emulsion Technology. Paul B (ed). Marcel Dekker. Inc., New York, NY, USA (1983)
  17. Hyndman CL, Groboillot AF, Poncelet D, Champagne CP, Neufeld RJ. Microencapsulation of Lactococus lactis within cross linked gelatin membrans. J. Chem. Technol. Biotechnol. 56: 259-263 (1993)
  18. Cordray JC, Huffman DL. Restructured pork form hot processed sow meat: Effect of encapsulated food acids. J. Food Prot. 48: 965-968 (1985) https://doi.org/10.4315/0362-028X-48.11.965
  19. Iso M, Masumoto H, Urushiyama S, Omi S. Application of encapsulated enzyme dispersed in a continuous stirred tank reactor. J. Microencapsul. 7: 167-177 (1990) https://doi.org/10.3109/02652049009021829
  20. Shin MG, Chang PS, Min BK, Kwak HS. Quantitative analysis of microencapsulation of ${\beta}$-galactosidase. Korean. J. Anal. Sci. 5: 471-475 (1992)