Prevalence and Characterization of Enterohemorrhagic Escherichia coli (EHEC) Isolated from Ground Beefs Distributed in Gyeong-In Region

경인지역에 유통되는 분쇄육 중 장출혈성대장균의 분포 조사 및 특성 연구

  • Kim, Eun-Jeong (Food Headquarters, Korea Food and Drug Administraion) ;
  • Park, Yong-Chjun (Testing Analysis Center, Gyeongin Regional Food and Drug Administration) ;
  • Cho, Joon-Il (Testing Analysis Center, Gyeongin Regional Food and Drug Administration) ;
  • Lee, Jong-Ok (Food Headquarters, Korea Food and Drug Administraion) ;
  • Kim, Hee-Yun (Testing Analysis Center, Gyeongin Regional Food and Drug Administration)
  • 김은정 (식품본부) ;
  • 박용춘 (경인지방식품의약품안정청 시험분석센터) ;
  • 조준일 (경인지방식품의약품안정청 시험분석센터) ;
  • 이종옥 (식품본부) ;
  • 김희연 (경인지방식품의약품안정청 시험분석센터)
  • Published : 2006.12.31

Abstract

The objective of this study was to evaluate three verocytotoxin-producing Escherichia coli (VTEC) detection kits to detect the presence of VT genes: Doupath Verocytotoxin (GLISA) developed by MERCK, ProsPect Shiga Toxin E. coil (STEC) Microplate Assay (ELISA) developed by Remel, and a polymerase chain reaction method. Our laboratory verified artificially inoculated samples. All three methods could detect very low numbers of VTEC, but VT-PCR had the best sensitivity for VTEC detection. From April through September 2005, 257 ground-beefs from supermakets and traditional markets were examined for the presence of VTEC by polymerase chain reaction immediately after purchase and total viable counts (TVC) were determined. VTEC was isolated from 30 of 257 ground-beefs. A variety of serogroups was found, including 10 stains belonging to the virulence type EHEC, but major serogroups such as O157, O26 and O111 were nor found.

Keywords

EHEC;PCR;ELISA;GLISA;prevalence

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