Evaluation of Antimicrobial Activity and Mutagenicity of Ozonized Olive Oil

오존화 올리브유의 항균성 및 변이원성 평가

  • Published : 2006.12.31

Abstract

Ozonized olive oil was tested for its mutagenic potential in a Salmonella/microsome assay. Additionally, antimicrobial activity was tested against Propionibacterium acnes, Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa, pathogenic strains related to acne, using the paper disk and agar dilution method. Ozonized olive oil showed antimicrobial activities against all the strains tested, with minimal inhibitory concentrations (MICs) values in a range of 2${\sim}$10 mg/mL. Mutagenicity of ozonized olive oil was evaluated with Salmonells typhimurium TA98, TA100 and Ta1535, with and without addition of S9 mixture. No increase in the number of $his^{+}$ revertants over the negative control (solvent and non-ozonized olive oil) values was observed with TA98 (1,000 ${\mu}g/plate$), TA100 (1,500 ${\mu}g/plate$) and TA1535 (1,500 ${\mu}g/plate$) strains. The results from this study suggested that ozonized olive oil does not show any mutagenic potential.

Keywords

ozonized olive oil;antimicrobial activity;acne;mutagenicity;Ames test

References

  1. Federal Register. Secondary direct food additives permitted in food for human consumption. Federal Register 66. 123: 33829-33830 (2001)
  2. Miura T, Suzuki S, Sakurai S, Matsumoto A, Shinriki N. Structure elucidation of ozonated olive oil. pp. 72-76. In: Proceedings of the 15th Ozone World Congress. September 11, Imperial College, London, UK. International Ozone Association, Scottsdale, AZ, USA (2001)
  3. Kwak HS, Nam CH, Kwon, HS. A study on the essential oil for hair health care. Korean Public Health Research 31: 27-36 (2005)
  4. NCCLS. Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically, 3th edn. Approved Standards, NCCLS document M7-A3. National Committee for Clinical Laboratory Standards, Villanova, PA, USA (1993)
  5. Lezcano N, Nunez M, Espino M, Gomez M. Antibacterial activity of ozonized sunflower oil, Oleozon, against Staphylococcus aureus and Staphylococcus epidermidis. Ozone Sci. Eng. 22: 207-214 (2000) https://doi.org/10.1080/01919510008547221
  6. Uvaldi A. Relazione sulle prove di tossicita' acuta sub-acuta e di calcolo della DL 50 del prodotto $bioperoxoil^{\circledR}$ (Della Ditta Ozonoil s. 1.). Thesis, Universita' delgi studi Parma, Facota' Di Medicina Veterinaria, Parma, Emillia-Romagna, Italy (1998)
  7. Sechi LA, Lezcano I, Numez N, Espim M, Dupre I, Pinna A, Molicotti P, Fadda G, Zanetti S. Antibacterial activity of ozonized oil (Oleozon). J. Appl. Microbiol. 90: 279-284 (2001) https://doi.org/10.1046/j.1365-2672.2001.01235.x
  8. Ames BN, Maron DM. Revised methods for the S. typhimurium mutagenicity test. Mutat. Res. 113: 173-215 (1983) https://doi.org/10.1016/0165-1161(83)90010-9
  9. Miura T, Yamazaki A, Nochi H, Iamoto K. Mechanism of inflammation of ozonized olive oil. pp. 25-33. In: Proceedings of the 9th Symposium. April 18, Nagai Memorial Hall, Tokyo, Japan. Japan Research Association for the Medical & Hygienic Use of Ozone, Osaka, Japan (2004)
  10. Menendez S, Falcon L, Simon DL, Landa N. Efficacy of ozonized sunflower oil in the treatment of tinea pedis. Mycoses 45: 329-332 (2002) https://doi.org/10.1046/j.1439-0507.2002.00780.x
  11. Valacchi G, Fortino V, Bocci V. The dual action of ozone on the skin. Br. J. Dermatol. 153: 1096-1100 (2005) https://doi.org/10.1111/j.1365-2133.2005.06939.x
  12. Rodrigues KL, Cardoso CC, Caputo LR, Carvalho JCYT, Fiorini JE, Schneedorf JM. Cicatrizing and antimicrobial properties of an ozonized from sunflower seeds. Inflammopharmacology 12: 261-270 (2004) https://doi.org/10.1163/1568560042342275
  13. Wolf A. Eine medizinische verwendbarkeit des ozones. Dtsch. Med. Wschr. 311: 1-5 (1915)
  14. Kim JG, Yosef, AE, Chism GW. Use of ozone to inactivate microorganisms on lettuce. J. Food Safety 19: 17-34 (1999) https://doi.org/10.1111/j.1745-4565.1999.tb00231.x
  15. Cardoso CC, Macedo SB, Carvalho JCT. Azione dell,Olio ozinizzato ($Bioperoxoil^{\circledR}$) nelle lesisone chirurgiche dei modelli preclinici, Farm. Ter. (Int. J. Drug Ther.) 19: 56-60 (2002)
  16. Ong TM, Mukhtar M, Wolf CR, Zeiger E. Differential effects of cytochrome P450-inducers on promutagen activation capabilities and enzymatic activities of S-9 from rat liver. J. Environ. Pathol. Toxicol. 4: 55-60 (1980)
  17. NCCLS. Performance standards for antimicrobial disk susceptibility test, 6th edn. Approved Standards, NCCLS document M2-A6. National Committee for Clinical Laboratory Standards, Waye, PA, USA (1997)