Determination of Glucan from Mycellium and Its Activity against Methicillin-Resistant Staphylococcus Aureus

버섯균사체 배양액에 함유된 Glucan의 정량 및 항생제 내성억제 효과

  • 나승영 (우석대학교 한의과대학 본초학교실) ;
  • 오서진 (우석대학교 한의과대학 본초학교실) ;
  • 정승일 (우석대학교 한의과대학 본초학교실) ;
  • 주영승 (우석대학교 한의과대학 본초학교실) ;
  • 정영두 (신농생명공학) ;
  • 김홍준 (한국한의학연구원) ;
  • 김정희 (우석대학교 한의과대학 본초학교실)
  • Published : 2006.12.25

Abstract

Objective : This experimental study was performed to investigate the contents of glucan from mycellium and its activity against methicillin-resistant Staphylococcus(MRSA). Methods : A gel permeation chromatography method was develped for the determination and isolation of glucan in mycellium. Their structures were elucidated using ^1H-NMR$, ^{13}C-NMR$. Also, The antibacterial activity of the glucan against MRSA was estimathed by determining the minimum inhibitory concentration(MIC). Results : The contents of glucan in mycellium was $766{\pm}0.19$ mg/g. Glucan exhibited activity against MRSA, with an MIC values of 4 to 9 mg/mL. Conclusions : These findings suggested that glucan might be useful in controlling MRSA infections.

Keywords

glucan;mycellium;methicillin-resistant