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Inhibitory Effects of Soyeum Pharmacopuncture (SPP) on LPS-induced Inflammation Related Cytokine Expressions of RAW 264.7 cells

소염약침의 LPS로 유도된 RAW 264.7 세포의 염증성 사이토카인 발현에 미치는 영향

  • Yoon, Mi-Young (Department of Oriental Pathology, Oriental Medical School, Daejeon University) ;
  • Yoo, Hwa-Seung (East-West Cancer Center, Daejeon University Dunsan Oriental Hospital)
  • 윤미영 (대전대학교 한의과대학 한방병리학교실) ;
  • 유화승 (대전대학교 한의과대학 둔산한방병원 동서암센터)
  • Published : 2007.12.31

Abstract

Aim This study was done to investigate whether SPP has inhibitory effects on the activation of RAW 264.7 cells. Method In tumor necrosis factor-a (TNF-a)/ interleukin-1b (IL-1b) and IL-6, the mRNA expression of molecular indicators related to inflammatory changes of the Reumatoid Arthritis (RA) were examined using quantitative real-time PCR. Results The treatment of SPP significantly suppressed the expression of proinflammatory cytokines and chemokines such as TNF-a, IL-1b, IL-6 compared with the control. The expression of NOS-II was considerably reduced, which was accompanied by a reduction in the production of nitric oxide (NO). It also reduced the expression of $TNF-{\alpha}$ in serum of Balb/c mice compared with control group. Conclusion SPP is an effective herbal material for suppressing the inflammation related cytokines of RAW 264.7 cells.

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