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Study on Anti-Allergic Effecst of Ganoderma lucidum Herbal Acupuncture and Ganoderma lucidum Extract

영지(靈芝) 약침(藥鍼)과 영지(靈芝) 추출액의 항알레르기 효과에 대한 연구

  • Kang, Kyung-Hwa (Dept. of Oriental Physiology, College of Oriental Medicine, Dong-Eui University) ;
  • Youn, Hyoun-Min (Dept. of Acupuncture, College of Oriental Medicine, Dong-Eui University)
  • 강경화 (동의대학교 한의과대학 생리학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구경혈학교실)
  • Published : 2007.12.31

Abstract

Objectives We studied on anti-allergic effects of Ganoderma lucidum herbal acupuncture(GHA) and Ganoderma lucidum extract(GE). Methods In vivo, Animals were herbal-acupunctured GHA at both B13s three times for 5 days. Then, we investigated compound 48/80-induced active systemic anaphylatic shock using ICR mice and anti-DNP IgE-induced passive cutaneous anaphylaxis using Sprague Dawley rat. In vitro, we measured cell viability, b-hexosaminidase release, IL-4 and TNF-a from RBL-2H3 cells, and nitric oxide from Raw264.7 cell after treatment of GE of various concentrations. Results In vivo, GHA pretreatments at both B13s inhibited compound 48/80-induced active systemic anaphylatic shock. Passive cutaneous anaphylaxis were inhibited by GHA10 and OP. In vitro, $0.1\;{\sim}\;2%$ GE treatments were not affect on cell viability and inhibited b-hexosaminidase release, IL-4, TNF-a and nitric oxide. Conclusions These results suggest that GHA and GE may be beneficial in the inhibition of allergic inflammatory response.

References

  1. 김창민 외. 중약대사전. 서울. 정담. 1999:3009-3012
  2. 大韓藥鍼學會. 약침요법 시술 지침서. 서울. 한성인쇄. 1999:13
  3. 丁奎萬. 알레르기와 韓方. 서울. 圖書出版第一路. 1993:59
  4. Dastych J, Walczak-Drzewiecka A, Wyczolkowska J, Metcalfe DD. Murine mast cells exposed to mercuric chloride release granule-associated N-acetyl-beta-dhexosaminidase and secrete IL-4 and TNFalpha. J Allergy Clin Immunol 1999;103:1108-1114 https://doi.org/10.1016/S0091-6749(99)70186-7
  5. Schwartz LB, Austen KF, Wasserman SI. Immunologic release of beta-hexosaminidase and beta-glucuronidase from purified rat serosal mast cells. J Immunol 1979;123:1445-1450
  6. Shiao,M.S., K.R Lee, J.J.Lin, and C.T.Wang, 1994. Phytochemicals for Cancer Prevention II, p.342. In C.T. Ho(eds), Teas, Spices and Herbs. American Chemical Society, Washington
  7. Kohguchi M, Kunikata T, Watanabe H, Kudo N, Shibuya T, Ishihara T, et al. Immunopotentiating effects of the antlershaped fruiting body of Ganoderma lucidum(Rokkaku-Reishi). Biosci Biotechnol Biochem. 2004;68:881-887 https://doi.org/10.1271/bbb.68.881
  8. Zhang QH, Lin ZB. Effect of Ganoderma lucidum polysaccharides B on TNF${\alpha}$ and INF${\gamma}$ production and their mRNA expression. J Beijing Med Univ. 1999;31:179-183
  9. Tang QJ, Zhang JS, Pan YJ, Werner R, Fan H. Activation of mouse macrophages by the alkali-extracted polysaccharide from spore of Ganoderma lucidum. Chin J Cell Mol Immunol. 2004;20:142-144
  10. Liu YH, Tsai CF, Kao MC, Lai YL, Tsai JJ. Effectiveness of Dp2 nasal therapy for Dp2-induced airway inflammation in mice: using oral Ganoderma lucidum as an immunomodulator. J Microbiol Immunol Infect. 2003;36(4):236-242
  11. White, D.H., K. Pluzmik and T. Ishizaka. Antigen-induced increase in protein kinase C activity in plasma membrane of mast cells. Proc. Nati. Acad. Sci. USA 1986:82:8139-8197 https://doi.org/10.1073/pnas.82.23.8139
  12. 김형민. 면역과 알레르기. 서울, 신일상사. 1998:159-173
  13. Marquardt DL, Wasserman SI. Modulation of rat serosal mast cell biochemistry by in vivo dexamethasone administration. J Immunol. 1983:131(2):934-939
  14. Mastuda H, Morikawa T, Ueda K, Managi H, Yoshikawa M. Structural requirements of flavonoids for inhibition of antigen-Induced degranulation, TNF-alpha and IL-4 production from RBL-2H3 cells. Bioorg Med Chem. 2002;10(10):3123-3128 https://doi.org/10.1016/S0968-0896(02)00227-4
  15. Stankiewicz W, Dabrowski MP, Chcialowski A, Plusa T. Cellular and cytokine immunoregulation in patients with chronic obstructive pulmonary disease and bronchial asthma. Mediators Inflamm 2002;11(5):307-312 https://doi.org/10.1080/09629350210000015719
  16. 이중달. 그림으로 설명한 병리학. 서울, 고려의학. 1991:29
  17. Gilchrist, M., Savoie, M., Nohara, O., Wills, F. L., Wallace, J. L., and Befus, A. D. J. Leukocyte Biol. 2002;71:618-624
  18. Careau E, Sirois J, Bissonnette EY. Characterization of lung hyperresponsiveness, inflammation, and alveolar macrophage mediator production in allergy resistant and susceptible rats. Am J Respir Cell Mol Biol. 2002;26(5):579-586 https://doi.org/10.1165/ajrcmb.26.5.4737
  19. S. Katayama, H. Shionoya and S. Ohtake: A new method for extraction of extravasated dye in the skin and the influence of fasting stress on passive cutaneous allergy in guinea pigs and rats. Microbiol. Immunol. 1978;22:89-101 https://doi.org/10.1111/j.1348-0421.1978.tb00352.x
  20. Jiang ZY, Lin C. Study of Ganoderma lucidum polysaccharide on effects of cellular immune function in mice. J Microbiol. 2003,23:51-54
  21. Dainaka J, Ichikawa A, Koibuchi Y, Nakagawa M, Tomita K. Effect of the tridecamer of compound 48/80, a $Ca^{2+}$- dependent histamine releaser, on phospholipid metabolism during the early stage of histamine release from rat mast cells. Biochem Pharmacol 1986;35(21):3739-3744 https://doi.org/10.1016/0006-2952(86)90659-3
  22. Iikura M, Yamaguchi M, Hirai K, Miyamasu M, Yamada H, Nakajima T, Fujisawa T, Ra C, Morita Y, Yamamoto K. Regulation of surface FcepsilonRI expression on human eosinophils by IL-4 and IgE. Int Arch Allergy Immunol. 2001;124(4):470-477 https://doi.org/10.1159/000053782

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