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Immatured Type Uterotrophic Assay for Estrogenicity Evaluation of DEHA

미성숙 랫드 자궁비대반응시험을 이용한 DEHA의 내분비계 장애작용 평가

  • Park, Ki-Dae (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Han, Beom-Seok (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Jeong, Ja-Young (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Oh, Jae-Ho (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Cho, Wan-Seob (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Cho, Min-Jeong (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Choi, Mi-Na (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Kim, Sung-Joon (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Kim, Seung-Hee (Korea Food and Drug Administration, National Institute of Toxicological Research)
  • 박기대 (식품의약품안전청 국립독성과학원) ;
  • 한범석 (식품의약품안전청 국립독성과학원) ;
  • 정자영 (식품의약품안전청 국립독성과학원) ;
  • 오재호 (식품의약품안전청 국립독성과학원) ;
  • 조완섭 (식품의약품안전청 국립독성과학원) ;
  • 조민정 (식품의약품안전청 국립독성과학원) ;
  • 최미나 (식품의약품안전청 국립독성과학원) ;
  • 김성준 (식품의약품안전청 국립독성과학원) ;
  • 김승희 (식품의약품안전청 국립독성과학원)
  • Published : 2007.10.30

Abstract

This study was aimed to investigate the estrogenic activity of Di-(2-ethylhexyl) adipate (DEHA) using immatured type uterotrophic assay. SD rats were treated with DEHA (40, 200, 1000mg/kg/day), estradiol-3-benzoate (EB) $(1{\mu}g/kg/day)$ as positive control on the assay. In immatured-type uterotrophic assay, relative organ weights of kidney and reproductive organs such as ovary at high-dose group were significantly increased compared to those of vehicle control group. DEHA did not influence the levels of serum FSH and LH, and uterine morphological changes such as luminal epithelial height, myometrial thickness and numbers of uterine gland, and BrdU indices. In these results, there was no significant variation by DEHA treatment, suggesting that DEHA appears not to be a endocrine disrupter with estrogenic activity.

Keywords

DEHA;immatured type uterotrophic assay;estrogenic activity

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