Initial Bacterial Groups in the Development of Biofilm in Drinking Water

수돗물속 생물막 형성의 초기 세균

  • Lee, Dong-Geun (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
  • 이동근 (신라대학교 의생명과학대학 제약공학과)
  • Published : 2007.10.30


To clarify the pioneer group in the development of biofilms in high chlorine residual water, a semi-pilot model system was operated and 16S rDNA V3 targeted PCR-DGGE was submitted. Biofilm formation occurred rapidly in the model of a drinking water distribution system. It reached $10^3\;CFU/cm^2$ or more on the surface of stainless steel, PVC, and galvanized iron in chlorinated (1.0 mg/l) water within a week. Within a week, uncultured Proteobacteria- and Bacillales group-like sequences were detected and Sphingomonas-like sequences were identified from all season and all pipe materials tested. Hence Sphingomonas species were regarded as the potential pioneer group in the development of biofilm in drinking water and this results would be useful for the prevention of biofilm formation and safety of drinking tap water.


biofilm;drinking water;DGGE;Sphingomonas sp


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