A Survey of Total Aflatoxins in Food Using High Performance Liquid Chromatography-Fluorescence Detector (HPLC-FLD) and Liquid Chromatography Tandem Mass Spectrometry(LC-MS/MS)

HPLC-FLD 및 LC-MS/MS에 의한 식품 중 총아플라톡신 오염실태 조사

  • 장미란 (부산지방식품의약품안전청 시험분석센터) ;
  • 이창희 (부산지방식품의약품안전청 시험분석센터) ;
  • 조성혜 (부산지방식품의약품안전청 시험분석센터) ;
  • 박준식 (부산지방식품의약품안전청 시험분석센터) ;
  • 권은영 (부산지방식품의약품안전청 시험분석센터) ;
  • 이은진 (부산지방식품의약품안전청 시험분석센터) ;
  • 김소희 (부산지방식품의약품안전청 시험분석센터) ;
  • 김대병 (부산지방식품의약품안전청 시험분석센터)
  • Published : 2007.10.31


A survey for total aflatoxins (aflatoxins $B_1$, $B_2$, $G_1$, and $G_2$) was conducted on 245 cereals and processed cereal products, and 148 nuts and processed nut products in Korea, for a total of 393 commercialized ed samples. The total aflatoxins were quantified by the immunoaffinity column clean-up method with high performance liquid chromatography (HPLC) - fluorescence detection (FLD), and were confirmed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Total aflatoxins(AFs) were detected in 37 samples (9.4% incidence), including 2 millet samples, 1 mixed cereal (sunsik), 1 powdered malt sample, 2 processed cereal products, 6 peanut samples, 22 peanut butter samples, and 1 sample each of almonds, adlay tea, and a processed nut product. The contamination levels were $0.04-2.65{\mu}g/kg$ for aflatoxin $B_1$, and $0.04-5.51{\mu}g/kg$ for total aflatoxins. Finally, LC-MS/MS analysis of the contaminated samples was conducted to confirm the detected aflatoxins, and all 37 samples showing aflatoxins by HPLC-FLD were confirmed by LC-MS/MS.


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