Antimicrobial Activity of Specific IgY against Salmonella gallinarum

Salmonella gallinarum에 대한 specific IgY의 항균력

  • Published : 2007.10.31


In this study, we investigated the antimicrobial effects of anti-Salmonella gallinarum-specific IgY separated from egg yolk obtained from layers immunized by S. gallinarum. The comparison tests of vaccination, content of IgY and innoculation number were examined by microscopic observation, turbidity, and pH. The results show that the ratio of anti-S. gallinarum IgY in the total IgY was 23%. Also, the anti-S. gallinarum IgY had selectivity only to S, gallinarum. The 0.3% addition of anti-S. gallinarum-specific IgY resulted in agglutinating clusters of S, gallinarum cells, but the agglomeration didn#t occur in IgY from layers not immunized nor in the control group. Microscopic observation indicated agglomerative cells when IgY was added at 0.2% or higher, and the pH and turbidity examinations revealed that a suppression effect was remarkable in IgY at more than 0.1%. These results suggest the possibility that IgY extracted from eggs and obtained from layers immunized by S. gallinarum can be used to prevent fowl typhoid.


Salmonella gallinarum;specific IgY;antimicrobial activity;egg yolk


  1. Ebina T, Satto A, Umeju K, Ohyama S, Oizumi A, Aikawa A, Katagiri S, Katagiri S, Suzuki H, Konno T. Prevention of rotavirus infection by oral administration of cow colostrum containing anti-human rotavirus anti body. Med. Microbiol. Immun. 174: 177-185 (1989)
  2. Yolken RH, Leister F, Wee S, Miskuff B, Vonderfecht S. Antibodies to rotavirus in chickens' eggs: A potential source of antiviral immunoglobulins suitable for human consumption. Pediatrics 81: 291-295 (1988)
  3. Arasteh N, Aminirissehe AH, Yousif AN, Albright LJ, Durance TD. Passive immunization of rainbow trout (Oncorhynchus mykiss) with chicken egg yolk immunoglobulins (IgY). Aquaculture 231: 23-36 (2004)
  4. Nilsson E, Amini A, Wretlind B, Larsson A. Pseudomonas aeruginosa infections are prevented in cystic fibrosis patients by avian antibodies binding Pseudomonas aeruginosa flagellin. J. Chromatog. B. (2007)
  5. Takashi T, Kenji T, Kouji S, Shigeo F. Separation of anti-E. coli IgY, water soluble proteins from egg yolk and its specificity against E. coli. Nippon Shokuhin Kag. Kasishi 42: 2259-229 (1995)
  6. Bartz CR, Conkin RH, Tunstall CB, Steele JH. Prevention of murine rotavirus infection with chicken egg yolk immunoglobulins. J. Infect. Dis. 142: 439-441 (1980)
  7. Larsson A, Balow RM, Linda TL, Forsberg PO. Chicken antibodies; taking advantage of evolution-A review. Poultry Sci. 72:1807-1812 (1995)
  8. Gutierrez MA, Miyazaki T, Hatai H, Kim M. Protective properties of egg yolk IgY containing anti-Edwardsiella tarda antibody against paracolo disease in the Japanese eel, Anguilla kaponica temminck & schlegel. J. Fish Dis. 16: 113-120 (1993)
  9. Fu CY, Huang H, Wang XM, Liu YG, Wang ZG, Cui SJ, Gao HL, Li Z, Li JP, Kong XG. Preparation and evaluation of anti- SARS coronavirus IgY from yolks of immunized SPF chickens. J. Virol. Methods 133: 112-115(2006)
  10. Baek BS, Han JP, Bae MJ. Properties and antimicrobial activity of egg yolk antibody against food poisoning bacteria. J. East Asian Soc. Dietary Life 9: 207-213 (1999)
  11. Jung SH, Kim HJ, Lyoo YS, Rho JH, Lee NH. Effect of anti- Helicobacter pylori IgY power to protect mice from Helicobacter pylori. Korea J. Food Sci. Technol. 38: 93-98 (2006)
  12. Hamada S, Horikoshi T, Minami T, Kawabata S, Hiraoka J, Fujiwara T, Ooshima T. Oral passive immunization against dental caries in rats by use of hen egg yolk antibodies specific for cellassociated glucosyltransferase of Streptococcus mutans. Infect. Immun. 59: 4161-4167 (1991)
  13. Hatta H, Tsuda K, Akachi S, Kim M, Yamamoto T. Oral passive immunization effect of anti-human rotavirus IgY and its behavior against proteolytic enzymes. Biosci. Biotech. Bioch. 57: 1077- 1081 (1993)
  14. Lee K, Ametani A, Shimizu M, Hatta H, Yamamoto T, Kimnogawa S. Production and characterization of anti-human insulin antibodies in the hen's egg. Agr. Biol. Chem. Tokyo 55: 2141- 2143. (1991)
  15. Rho JH, Kim, YB, Han CK, Lee NH, Sung KS, Shon DH. The effect of age of hens and vaccincation on anti-Strepteococcus mutans IgY level. Korean J. Sci. 41: 563-574 (1999)
  16. Lee NH, Rho JH, Han CK, Sung KS. Effect of various hen feed supplements on IgY level in eggs and laying rates. Korean J. Anim. Sci. 41: 155-166 (1999)
  17. Kim YB, Rho JH, Han CK, Sung KS, Son DH, Kim YD, Lee SH, Ha KJ. Development of specific IgY order to replace antibiotics in prevention and treatment of flounder's disease. Korea Food Res. Inst., Seongnam, Korea. pp. 66-104 (2001)
  18. Shon DH, Rho JH, Kim YB, Han CK, Sung KS, Lee NH. Properties of anti-S. mutans IgY separated form egg yolk. Korean J. Food Sci. Technol. 30: 1029-1034 (1998)
  19. Kim YB, Rho JH, Shon DH, Kim JH, Sung KS, Lee NH. Antimicrobial activity of specific IgY against Strepteococcus mutans. Korean J. Food Sci. Technol. 32: 1319-1325 (2000)
  20. Hatta H, Kim M, Yamamoto T. A novel isolation method for hen egg yolk antibody 'IgY'. Agr. Biol. Chem. Tokyo 54: 2531-2535 (1990)
  21. Rho JH, Kim MH, Kim YB, Sung KS, Lee NH. Formation and processing properties of anti-Salmonella gallinarum specific IgY from yolk. Korean J. Anim. Sci. 47: 637-646 (2005)
  22. Kobayashi C, Yokoyama H, Nguyen SV, Kodama Y, Kimata T, Izeki M. Effect of egg yolk antibody on experimental Cryptosporidium parvum infection in scid mice. Vaccine 23: 232-235 (2004)
  23. Rho JH, Kim YB, Han CK, Sung KS, Chi CH. Development of IgY against Salmonella gallinarum using layers. Korea Food Res. Inst., Seongnam, Korea. pp. 21-52 (2002)
  24. Patterson R, Younger JS, Weigle WO, Dixon FJ. The metabolism of serum proteins in the hen and chick and secretion of serum proteins by the ovary of the hen. J. Gen. Physiol. 45: 501-513 (1962)
  25. Shimizu M, Fitzsimmons R, Nakai S. Anti-E. coli immunoglobulin Y isolated from egg yolk immunized chickens as a potential food ingredient. J. Food Sci. 53: 1360-1366 (1998)
  26. Lee SC. The feed for chicken used hardwood charcoals and herbs. Korea Patent 10-2004-0064557 (2004)
  27. Amaral JA, Franco MT, Carneiro-sampaio MMS, Carbonare SB. Anti-enteropathogenic Escherichia coli immunoglobulin Y isolated from eggs laid by immunised Leghorn chickens. Re. Vet. Sci. 72: 229-234(2007)