Antimicrobial Effect of Achyranthes japonica Nakai Extracts against Clostridium difficile

우슬 추출물의 Clostridium difficile에 대한 항균 효과

  • Jung, Sun-Mi (Department of Biological Engineering, Inha University) ;
  • Choi, Soo-Im (Department of Biological Engineering, Inha University) ;
  • Park, Sang-Min (Department of Biological Engineering, Inha University) ;
  • Heo, Tae-Ryeon (Department of Biological Engineering, Inha University)
  • 정선미 (인하대학교 생물공학과) ;
  • 최수임 (인하대학교 생물공학과) ;
  • 박상민 (인하대학교 생물공학과) ;
  • 허태련 (인하대학교 생물공학과)
  • Published : 2007.10.31


In this study, the ethanolic extracts of 40 species of traditional herbal medicines were examined for their antimicrobial activities against Clostridium difficile. Among the 43 screened traditional herbal medicines, Achyranthes Japonica Nakai (AJN), Siegesbeckia glabrescens Makino, and Phelloedendron amurense Ruprecht showed antimicrobial activities greater than 90% at a concentration of 500 ppm. According to the minimum inhibitory concentration (MIC) test the ethyl acetate soluble fraction of the AJN ethanolic extracts (AJNEA) showed the highest growth inhibitory activity against C. difficile, with a MIC of $625{\mu}g/mL$. In addition, the effect of AJNEA on the growth of lactic acid bacteria was investigated. AJNEA did not inhibit the growth of the tested Bifidobacterium spp. or Lactobacillus spp., with the exception of B. longum, Streptococcus thermophilus, and L. helveticus. These results indicate that AJNEA can be utilized as a potential antimicrobial agent against C. difficile related disease.


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