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Differentiation in Feeding Behaviour of Biotypes B and Q of Bemisia tabaci (Homoptera: Aleyrodidae) against Three Insecticides

3종의 살충제에 대한 담배가루이 Biotype B와 Q의 섭식행동 비교

  • Seo, Mi-Ja (Dept. of Plant Medicine, College of Agricultural, Life, and Environment, Chungbuk National University) ;
  • Yang, Jeong-Oh (Dept. of Plant Medicine, College of Agricultural, Life, and Environment, Chungbuk National University) ;
  • Yoon, Chang-Mann (Dept. of Plant Medicine, College of Agricultural, Life, and Environment, Chungbuk National University) ;
  • Youn, Young-Nam (Dept. of Applied Biology, College of Agricultural and Life Science, Chungnam National University) ;
  • Kim, Gil-Hah (Dept. of Plant Medicine, College of Agricultural, Life, and Environment, Chungbuk National University)
  • 서미자 (충북대학교 농업생명대학 식물의학과) ;
  • 양정오 (충북대학교 농업생명대학 식물의학과) ;
  • 윤창만 (충북대학교 농업생명대학 식물의학과) ;
  • 윤영남 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 김길하 (충북대학교 농업생명대학 식물의학과)
  • Published : 2007.12.30

Abstract

The feeding behaviors of 2 biotypes (type B and Q) of tobacco whitefly, Bemisia tobaci, were monitored using EPG technique on tomato and pepper plants treated 3 insecticides for controlling whiteflies, for examples, acetamiprid, spinosad and thiamethoxam. After treatment of three insecticides with recommended concentrations to tomato and pepper plants, EPG waveforms were recorded during 6 hours. The characteristic patterns of feeding behaviors investigated were as follows; time consumed by withdrawal of proboscis, total non-penetration time, total stylet pathway pattern time and total phloem feeding time. There was somewhat difference among 3 insecticides tested. As a result of investigation of total duration showed the stylet pathway activity due to the reaction against all tested insecticides, the Q biotype showed fewer time than those from the B biotype. The B biotype showed more frequent stylet pathway activity patterns during whole recording time and a shorter phloem ingestion time than those from the Q biotype. In result of prior up (non-penetration) time representing the reaction against the insecticide treated, the time of B biotype was more faster than that of the Q biotype, so it was considered that the B biotype was more sensitive to the tested insecticides. Therefore, our results revealed a clear difference in feeding behaviour between the B and Q biotypes of B. tabaci. Also, it was investigated that B biotype was susceptible to the 3 insecticides.

Acetamiprid, spinosad, thiamethoxam 3종류의 살충제에 대해 담배가루이 biotype B와 Q 타입간의 섭식행동을 EPG기법으로 분석하였다. 3종류의 살충제를 추천농도로 처리한 후 6시간동안 기록한 EPG waveform을 가지고 약제에 반응하여 최초로 구침을 빼기까지 소요된 시간, 전체 기록시간동안의 구침을 빼고 있는 시간, 구침활성이 일어나는 시간, 직접적인 섭식행동인 체관부 섭식시간 등을 조사, 비교분석하여 타입별 살충제에 대한 영향을 평가하였다. 살충제간 약간의 차이를 보였으나, 3가지 살충제 모두 기록 시작 후 살충제에 반응하여 구침의 활성에 변화를 보이는 전체시간을 측정한 결과, B biotype이 Q biotype에 비해 구침의 활성시간이 높게 나타났으며, 체관부를 흡즙하는 시간은 반대로 적게 나타났다. 살충제에 반응하여 구침을 빼기까지 소요된 시간은 B타입이 Q타입보다 빨리 나타났는데, 이는 살충제에 좀 더 빠르게 반응한 것으로 보인다. 결과적으로 담배가루이 biotype B는 Q타입보다 식물체에 처리된 살충제를 빠르게 감지하며 섭식을 덜 함으로써 3가지 살충제에 대해 감수성 경향을 보이는 것으로 확인되었다.

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