Differentiation in Feeding Behaviour of Biotypes B and Q of Bemisia tabaci (Homoptera: Aleyrodidae) against Three Insecticides

3종의 살충제에 대한 담배가루이 Biotype B와 Q의 섭식행동 비교

  • Seo, Mi-Ja (Dept. of Plant Medicine, College of Agricultural, Life, and Environment, Chungbuk National University) ;
  • Yang, Jeong-Oh (Dept. of Plant Medicine, College of Agricultural, Life, and Environment, Chungbuk National University) ;
  • Yoon, Chang-Mann (Dept. of Plant Medicine, College of Agricultural, Life, and Environment, Chungbuk National University) ;
  • Youn, Young-Nam (Dept. of Applied Biology, College of Agricultural and Life Science, Chungnam National University) ;
  • Kim, Gil-Hah (Dept. of Plant Medicine, College of Agricultural, Life, and Environment, Chungbuk National University)
  • 서미자 (충북대학교 농업생명대학 식물의학과) ;
  • 양정오 (충북대학교 농업생명대학 식물의학과) ;
  • 윤창만 (충북대학교 농업생명대학 식물의학과) ;
  • 윤영남 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 김길하 (충북대학교 농업생명대학 식물의학과)
  • Published : 2007.12.30


The feeding behaviors of 2 biotypes (type B and Q) of tobacco whitefly, Bemisia tobaci, were monitored using EPG technique on tomato and pepper plants treated 3 insecticides for controlling whiteflies, for examples, acetamiprid, spinosad and thiamethoxam. After treatment of three insecticides with recommended concentrations to tomato and pepper plants, EPG waveforms were recorded during 6 hours. The characteristic patterns of feeding behaviors investigated were as follows; time consumed by withdrawal of proboscis, total non-penetration time, total stylet pathway pattern time and total phloem feeding time. There was somewhat difference among 3 insecticides tested. As a result of investigation of total duration showed the stylet pathway activity due to the reaction against all tested insecticides, the Q biotype showed fewer time than those from the B biotype. The B biotype showed more frequent stylet pathway activity patterns during whole recording time and a shorter phloem ingestion time than those from the Q biotype. In result of prior up (non-penetration) time representing the reaction against the insecticide treated, the time of B biotype was more faster than that of the Q biotype, so it was considered that the B biotype was more sensitive to the tested insecticides. Therefore, our results revealed a clear difference in feeding behaviour between the B and Q biotypes of B. tabaci. Also, it was investigated that B biotype was susceptible to the 3 insecticides.


  1. Bharathan, N.G., W.R. Graves, K.R. Narayanan, D.J. Schusted, H.H.Bryan and R.T. McMillan. 1990. Association of doublestranded RNA with whitefly-mediated sivering in squash. Plant Pathol. 39: 530-538
  2. Cohen, S., J.E. Duffus and H.Y. Kim. 1992. A new Bemisia tabaci biotype in the southwestern United States and its role in siiverleaf of squash and transmission to lettuce infectious yellow virus. Phytopathol. 82: 86-90
  3. Liu, M.Y. and J.E. Casida. 1993. High affinity binding of $[^3H]$ Imidacloprid labels high- and low-affinity nicotinic acetylcholine receptor-like binding sites in aphid Myzus persicae (Homoptera: Aphididae). Pestic. Biochem. Physiol. 62: 3-14
  4. Naeun, R. 1995. Behaviour modifying effects of low systemic concentrations of imidacloprid on Myzus persicae with special reference to an antifeeding response. Pest. Sci. 44: 145-153
  5. Nauen R., N. Stumpf and A. Elbert. 2002. Toxicological and mechanistic studies on neonicotinoid cross resistance in Q-type Bemisia tabaci. Pest Manag. Sci. 58: 868-875
  6. Rosell, R.C., I.D. Bedford, D.R. Frohlich, R.l Gill, J.K. Brown and P.G. Markham. 1997. Analysis of morphological variation in distinct populations of Bemisia tabaci (Homoptera: Aleyrodidae). Ann. Entomol. Soc. Am. 90: 575-589
  7. Chu, D., Y.J. Zhang, B. Cong, B.Y. Xu and Q.J. Wu. 2004. The invasive mechanism of a world important pest, Bemisia tabaci biotype B. Acta Ent. Sinica. 47: 400-406
  8. Horowitz, A.R., Gorman, K., G. Ross and I. Denholm. 2003. Inheritance of pyriproxyfen resistance in the whitefly, Bemisia tabaci (Q biotype). Arch. Insect Biochem. Physiol. 54: 177-186
  9. Tjallingii, W.F. 1988. Electrical recording of stylet penetration activities. Aphids, Their biology, Natural enemies and control, Vol. B (ed. By A.K. Minks and Harrewijn), pp. 98-108. Elsevier Science Publishers B.V., Amsterdam
  10. Jiang, Y.X. and G.P. Walker. 2003. Electrical penetration graphs of the nymphal stage of Bemisia argentifolii. Ent. Exp. Appl. 109: 101-111
  11. Lin, F.C., T.T. Hsieh and C.L. Wang. 2005. Occurrence of whiteflies and their integrated management in Taiwan. Proc. Intern. Seminar Whitefly Management and Control Strategy. 241-255
  12. Mound, L.A. and S.H. Halsey. 1978.whiteflies of the world. A systemic catalogue of the Aleyrodidae (Homoptera) with host plant and natural enemy data. London: British Museum of Natural History. Wiley. New York
  13. Rauch, N. and R. Nauen. 2003. Identification of biochemical markers linked to neonicotinoids cross resistance in Bemisia tabaci. Arch Insect Biochem. Physiol. 54: 165-176
  14. Jiang Y.X., H. Lei, J.L. Collar, B. Martin, M. Muniz and A. Fereres. 1999. Probing and feeding behaviour of two distinct biotypes of Bemisia tabaci (Homoptera: Aleyrodidae) on tomato plants. J. Econ. Entomol. 92: 357-366
  15. Bellows, T.S.J., T.M. Perring, R.J. Gill and D.H. Headrick. 1994. Description of a species of Bemisia (Homoptera: Aleyrodidae). Ann. Ent. Soc. Am. 87: 195-206
  16. Janssen, J.A.M., W.F. Tjallingii and J.C. van Lenteren. 1989. Electrical recording and ultrastructure of stylet penetration by the greenhouse whitefly. Ent. Exp. Appl. 52: 69-81
  17. Muniz, M. and G. Nombela. 1997. Host preference, pupae production and sex ratio of Bemisia tabaci (Homoptera: Aleyrodidae) on three varieties of Capsicum annuum L., pp. 1-5. In A. Hanaf (eds.), Proceedings of international symposium on integrated production and protection in horticultural crops. Agadir. Morocco
  18. Walker, G.P. and T.M. Perring. 1994. Feeding and oviposition behaviour of whiteflies (Homoptera: Aleyrodidae) interpreted from AC electronic feeding monitor waveforms. Ann. Ent. Soc. Am. 87: 363-374

Cited by

  1. Feeding behaviors of Cacopsylla pyricola (Hemiptera: Psyllidae) using electrical penetration graphs (EPGs) vol.43, pp.2, 2016,
  2. Feeding Behavior of the Green Peach Aphid, Myzus persicae, against Several Insecticides Using Electrical Penetration Graph Techniques vol.21, pp.2, 2017,
  3. Occurrence of Sweet-potato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) and Its Response to Insecticide in Gyeonggi Area vol.51, pp.4, 2012,
  4. Feeding response of the cotton aphid,Aphis gossypii, to sublethal rates of flonicamid and imidacloprid vol.154, pp.2, 2015,
  5. Changed in Feeding Behavior of Cacopsylla pyricola Foerster(Hemiptera: Psyllidae) and Activities of Several Insecticides vol.20, pp.1, 2016,