Evaluation of Selective Media for Isolation of Foodborne Bacteria

주요 식중독균 분리용 선택배지의 타당성 연구

  • Jo, Seo-Hee (Department of Food Science and Technology, Chung-Ang University) ;
  • Ha, Ji-Hyoung (Department of Food Science and Technology, Chung-Ang University) ;
  • Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University) ;
  • Shim, Young-Hwan (Department of Food Science and Biotechnology and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Kwon, Ki-Sung (Food Microbiology Division, Korea Food & Drug Administration) ;
  • Han, Jeong-A (Food Microbiology Division, Korea Food & Drug Administration) ;
  • Hwang, In-Gyun (Food Microbiology Division, Korea Food & Drug Administration) ;
  • Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University) ;
  • Oh, Deog-Hwan (Department of Food Science and Biotechnology and Institute of Bioscience and Biotechnology, Kangwon National University)
  • 조서희 (중앙대학교 식품공학과) ;
  • 하지형 (중앙대학교 식품공학과) ;
  • 김근성 (중앙대학교 식품공학과) ;
  • 심영환 (강원대학교 식품생명공학과) ;
  • 권기성 (식품의약품안전청 식품미생물팀) ;
  • 한정아 (식품의약품안전청 식품미생물팀) ;
  • 황인균 (식품의약품안전청 식품미생물팀) ;
  • 하상도 (중앙대학교 식품공학과) ;
  • 오덕환 (강원대학교 식품생명공학과)
  • Published : 2007.12.31

Abstract

This study was conducted to evaluate the selective media listed in currently available Food Code in Korea. The 29 different types of media of five different types of foodborne bacteria including Salmonella spp., Bacillus cereus, Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus were tested in the broth and food. The recovery test for five different types of foodborne bacteria was performed in the artificially inoculated into chicken, rice, pork and mackerel. There was no significant differences in isolation capabilities among twenty nine different types of isolation selective media for five different types of foodborne bacteria in broth condition, while there was significantly a little differences in isolation capabilities among those on foods (P<0.05). The higher number of foodborne pathogens were isolated from conventional selective media approved in Food Code than newly developed selective media such as chromogenic media. This results suggest that there was differences of selectivities among currently available isolation selective media in many countries and further studies are needed to be approved by Korean Food and Drug Administration.

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