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Solubilization of Insoluble Phosphates by Aspergillus sp. PS-104 Isolated from Soil

토양에서 분리한 Aspergillus sp. PS-104 균주에 의한 난용성 인산염 분해

  • Published : 2007.03.27

Abstract

Phosphate-solubilizing microorganisms were isolated from soil around Kyungnam and Kyungbook regions using potato dextrose agar-calcium phosphate medium. A fungus with the greatest phosphate-solubilizing activity was selected and identified to Aspergillus sp. PS-104, based on the morphological characteristics of conidiophore and conidia; unbranching type of conidiophore, terminally swelling of conidiophore and septate of mycelium, in malt extract agar and potato dextrose agar media. The optimum temperature and initial pH to solubilize rock phosphate in potato dextrose broth-rock phosphate medium were $30^{\circ}C$ and pH 7.0, respectively. In these optimum conditions, phosphate-solubilizing activities of Aspergillus sp. PS-104 against four twos of insoluble phosphate, tricalcium phosphate, aluminium phosphate, hydroxyapatite and rock phosphate, were quantitatively determined. As result, the maximum phosphate-solubilizing activity was obtained with tricalcium-phosphate (1,900 ppm) while minimum activity was obtained with hydroxyapatite (320 ppm). Futhermore, phosphate-solubilizing activity of Aspergillus sp. PS-104 was found higher when treated with nitrates as compared to the ammonium salts as a nitrogen sources.

References

  1. Paul, E. A. and Clark, F. E. (1989) Soil Microbiology and Biochemistry. Academic Press, New York, USA
  2. Illmer, P., Barbato, A. and Schirmer, F. (1995) Solubilization of hardly-soluble $AlP0_4$ with P-solubilizing microorganisms. Soil Biol. Biochem. 27, 265-270 https://doi.org/10.1016/0038-0717(94)00205-F
  3. Sayer, J. A., Raggett, S. L. and Gadd, G. M. (1995) Solubilization of insoluble metal compounds by soil fungi: Development of a screening method for solubilizing ability and metal tolerance. Mycological Res. 99, 987-993 https://doi.org/10.1016/S0953-7562(09)80762-4
  4. Varsha, N., Jugnu, T. and Patel, H. H. (1995) Mineral phosphate solubilization by Aspergillus aculeatus. Ind. J. Exp. Biol. 33, 91-93
  5. Choi, M. C. and Kang, S. C. (1999) Solid culture of phosphate-solubilizing fungus, Penicillium sp. PS-113. Kor. J. Appl. Microbiol. Biotechnol. 27, 1-7
  6. Kang, S. C. Ha, C. K., Lee, T. G. and Maheshwari, D. K. (2002) Solubilization of insoluble inorganic phosphate by a soil-inhabiting fungus Fonitopsis sp. PS 102. Curr. Sci. 82, 439-442
  7. Choi, M. C., Chung, J. B., Sa, T. M., Lim, S. U. and Kang, S. C. (1997) Solubilization of insoluble phosphates by Penicillium sp. GL-101 isolated from soil. Agric. Chem. Biotechnol. 40, 329-333
  8. Agasimani, C. A., Mudlagiriyappa and Sreenivasa, M. N. (1994) Response of groundnut to phosphate solubilizing microorganisms. Groundnut News 6, 5
  9. Illmer, P. and Schinner, F. (1995) Solubilization of inorganic calcium phosphates-solubilization mechanisms. Soil Biol. Biochem. 27, 257-263 https://doi.org/10.1016/0038-0717(94)00190-C
  10. Kucey, R. M. N. (1988) Effect of Penicillium bilaji on the solubility and uptake of P and micronutrients from soil by wheat. Can. J. Soil Sci. 68, 261-270 https://doi.org/10.4141/cjss88-026
  11. Dubey, S. K. and Billore, S. D. (1992) Phosphate solubilizing microorganism(PSM) as inoculant and their role in augmenting crop productivity in India - A review. Crop Res. Hisar. 5, 11
  12. Tiwari, V. N., Pathak, A N. and Lehri, L. K. (1993) Rock phosphate-superphosphate in wheat in relation to inoculation with phosphate solubilizing organism and organic waste. Ind. J. Agr. Res. 27, 137-145
  13. Varsha, N., Jugnu, T. and Patel, H. H. (1993) Solubilization of natural rock phosphates and pure insoluble inorganic phosphates by Aspergillus awamori. Ind. J. Exp. Biol. 31, 747-749
  14. Suh, J. S., Lee, S. K., Kim, K. S. and Seong, K. Y. (1995) Solubilization of insoluble phosphates by Pseudomonas putida, Penicillium sp. and Aspergillus niger isolated from Korean soils. J. Korean Soc. Soil Sci. Fert. 28, 278-286
  15. Hawksworth, D. L., Sutton, B. C. and Ainsworth, G. C. (1983) Ainsworth & Bisby's Dictionary of the Fungi(7th ed.). Commonwealth Mycological Institute. England