Selection of Fungicides for the Control of Soybean Black Root Rot Caused by Calonectria ilicicola

콩 검은뿌리썩음병 방제를 위한 살균제 선발

  • Park, Seong-Woo (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Kang, Beom-Kwan (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Kim, Hong-Sik (Department of Agronomy, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Woo, Sun-Hee (Department of Agronomy, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Kim, Heung-Tae (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
  • 박성우 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학) ;
  • 강범관 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학) ;
  • 김홍식 (충북대학교 농업생명환경대학 식물자원생산학부 식물자원학) ;
  • 우선희 (충북대학교 농업생명환경대학 식물자원생산학부 식물자원학) ;
  • 김흥태 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학)
  • Published : 2007.03.30

Abstract

Fungicidal screening was performed to control soybean black root rot caused by Calonectria ilicicola through in vitro and greenhouse assays. In in vitro assay, 25 fungicides were assessed by an agar dilution method and a 96-well microtiter plate method. While protective fungicides including dithianon, dichlofluanid, mancozeb, and captan showed a very low activity against the mycelial growth C. ilicicola SC03-15 in the agar dilution method, they displayed potent inhibitory activity against spore germination in a 96-well microtiter plate method with $EC_{50}$ values of 4.65, 0.61, 4.64, and $0.29{\mu}g\;mL^{-1}$, respectively. Ergosterol biosynthesis-inhibiting (EBI) fungicides showed different antifungal activity against mycelial growth and spore germination according to molecules. Difenconazole displayed higher antifungal activity against spore germination rather than mycelial growth, and prochloraz inhibited potently both mycelial growth and spore germination with EC50 values less than $1.8{\mu}g\;ml^{-1}$. In contrast, the other EBI fungicides inhibited more highly mycelial growth than spore germination. Carbendazim+diethofencarb and dazomet also inhibited both mycelial growth and spore germination of C. ilicicola SC03-15 at very low concentrations. In greenhouse assay, carbendazim+diethofencarb effectively controlled a soybean black root rot by drenching 2 days before or after inoculation. In addition, tebuconazole showed potent curative activity against soybean black root rot.

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