Herbicidal and Insecticidal Potentials of 5-Aminolevulinic acid, a Biodegradable Substance

생분해성 생리활성물질 5-aminolevulinic acid의 제초 및 살충활성

  • Chon, Sang-Uk (Callus Co., Ltd., TBI Center, Gwangju Institute of Science and Technology)
  • 천상욱 (광주과학기술원 창업기술지원센터 (주)캐러스)
  • Published : 2007.03.30

Abstract

ALA (5-aminolevulinic acid) has been proposed as a tetrapyrrole-dependent photodynamic herbicide and insecticide by the action of the protoporphyrinogen IX oxidase (Protox IX). The present study was conducted to determine growth responses of plant and insects to ALA, biodegradable biopesticidal substance. In the paddy condition experiment, plant height and shoot fresh weight of barnyardgrass (Echinochloa crus-galli) was more reduced by ALA than rice plants, even though both plant species show great phytotoxicity. Hairy crabgrass (Digitaria sanguinalis), a monocot weed, was more sensitive to ALA at 5mM under upland condition when ALA applied on the foliage, compared with soybean (Glycine max) as a dicot crop. ALA solutions were tested for their insecticidal and larvicidal activities against Spodaptera exigua (Hubner) and Tetranychus urticae Koch. by foliar application and leaf-dipping method. The result showed higher insecticidal activity of ALA at 10mM and its mixture with insecticide luferon against S. exigua. Strongest insecticidal activity against T. urticae was observed from the ALA solution at 10mM 72 days after application. This results show that ALA solution had potent herbicidal and insecticidal activities against agricultural pests even though their activities were lower than those of synthetic pesticides.

References

  1. Roy, C. B., and M. Vivekanandan (1998) Role of aminolevulinic acid in improving biomass production in Vigna catjung, V. mungo, and V. radiata. Biologia Plantarum 41:211 -215 https://doi.org/10.1023/A:1001806429035
  2. Tanaka, T., K. Takahashi, Y. Hotta and Y. Takeuchi (1992) 5-Aminolevulinic acid as plant growth stimulator. Eur. Pat. App. EP 541-776
  3. Hotta, Y., T. Tanaka, H. Takaoka, Y. Takeuchi and M. Konnai (1997a) New physiological effects of 5 aminolevulinic acid in plants: the increase of photosynthesis, chlorophyll content, and plant growth. Biosci. Biotech. Biochem. 61:2025-2028 https://doi.org/10.1271/bbb.61.2025
  4. Towers, G. H. N. and J. P. Arnason (1988) Photodynamic herbicides. Weed Technol. 2:545-549 https://doi.org/10.1017/S0890037X00032425
  5. Chereskin, B. M. and P. A. Castelfranco (1982) Effects of iron and oxygen on chlorophyll biosynthesis. 2. Observations on the biosynthetic pathway in isolated etiochloroplasts. Plant Physiol. 69: 112 -116 https://doi.org/10.1104/pp.69.1.112
  6. Sundquvist, C. (1969) Transformation of protochlorophyllide, formed from exogenous $\delta$-aminolevulinic acid in continuous light and flashlight. Physiol. Plant 22: 147-156 https://doi.org/10.1111/j.1399-3054.1969.tb07850.x
  7. Weinstein, J. D. and S. I. Beale (1985) Enzymatic conversion of glutamate to $\delta$-aminolevulinic acid in soluble extracts of the unicellular green alga, Chlorella vulgaris. Arch Biochem Biophys. 239:454-464
  8. Chon S. U. (2003) Herbicidal activity of $\delta$-aminolevulinic acid on several plants as affected by application methods. Korean J. Crop Sci. 48:50-55
  9. Johnson, W.O., G. E. Kollman, C. Swithenbank and R. Y. Yih (1978) RH-6201 (blazer): A new broad spectrum herbicide for postemergence use in soybeans. J. Agric. Food. Chem. 26:285 -286 https://doi.org/10.1021/jf60215a027
  10. Choi, C, B. S. Hong, H. C. Sung, H. S. Lee and J. H. Kim (1999) Optimization of extracellular 5-aminolevulinic acid production from Escherichia coli transformed with ALA synthase gene for Bradyrhizobium japonicum. Biotech. Letters. 21:551 -554 https://doi.org/10.1023/A:1005520007230
  11. Rebeiz, C. A., A. Montazer-Zouhoor, H. J. Jopen and S. M. Wu (1984) Photodynamic herbicides: Concept and phenomenology. Enzyme Microb. Technol. 6:390-401 https://doi.org/10.1016/0141-0229(84)90012-7
  12. Matsumoto, H., Y. Tanida and K. Ishizuka (1994) Porphyrin intermediate involved in herbicidal action of $\delta$-aminolevulinic acid on duckweed. Pestic. Biochem. Physiol. 48:214 -221 https://doi.org/10.1006/pest.1994.1022
  13. Dailey, H. A. (1990) Biosynthesis of heme and chlorophylls. McGraw-Hill Publishing Co. New York, USA, pp.594
  14. Duke, S. O., J. Lydon, J. M. Becerril, T. D. Sherman, L. P. Lehnen and H. Matsumoto (1991) Protoporphyrinogen oxidase-inhibiting herbicides. Weed Sci. 39:465 -473
  15. Chon S. U., S. Jung, H. O. Boo and S. K. Han (2006) Natural photodynamic activity of 5-aminolevulinic acid produced by an E. coli overexpressing ALA synthase from Bradyrhizobium japonicum. Koren J. Crop Sci. 51:356-361
  16. Hotta, Y., T. Tanaka, H. Takaoka, Y. Takeuchi and M. Konnai (1997b) Promotive effect of 5-aminolevulinic acid on the yield of several crops. Plant Growth Regulation 22:109-114 https://doi.org/10.1023/A:1005883930727
  17. Rebeiz, C. A., J. A. Juvik and C. C. Rebeiz (1988) Photodynamic insecticides I. Concept and phenomenology. Pesticide Biochem. Physiol. 30:11-27 https://doi.org/10.1016/0048-3575(88)90055-7
  18. Watanabe, K., T. Tanaka, Y. Hotta, H. Kuramochi and Y. Takeuchi. (2000) Improving salt tolerance of cotton seedlings with 5-aminolevulinic acid. Plant Growth Regulation 32:99-103