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Increased Antimutagenic and in vitro Anticancer Effects by Adding Green Tea Extract and Bamboo Salt during Doenjang Fermentation

녹차추출물 및 죽염처리에 의한 된장의 항돌연변이 및 in vitro 항암활성 증진효과

  • Hwang, Kyung-Mi (Hazard Substances Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Oh, Sung-Hoon (Dept. of Food Science and Biotechnology, Ansan College of Technology) ;
  • Park, Kun-Young (Dept. of Food Science and Nutrition, Pusan National University)
  • 황경미 (경인지방식품의약품안전청 유해물질분석반) ;
  • 오성훈 (안산공과대학 식품생명과학과) ;
  • 박건영 (부산대학교 식품영양학과)
  • Published : 2007.01.31

Abstract

Antimutagenic and in vitro anticancer effects of doenjangs added with green tea extract and/or using bamboo salt were studied by Ames test using Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) TA100 and 3-(4,5-dimethylthiazol) -2,5-diphenyltetrazolium bromide (MTT) assay on PC-3 and DU145 human prostate cancer cells, respectively. At the 1.25 mg/plate concentration, 1% green tea extract (GTE) added doenjang exhibited 85% antimutagenicity against aflatoxin $B_1$ ($AFB_1$), while the control doenjang revealed 63% antimutagenicity, showing increased antimutagenic effect by the addition of green tea extract during doenjang fermentation. GTE added doenjang also increased antimutagenic effect against MNNG. The inhibition rate of the control doenjang showed 34% at 0.625 mg/plate, while 1% and 2% GTE added doenjangs inhibited by 56% and 73% at the 0.625 and 1.25 mg/plate, respectively (p<0.05). In MTT assay, GTE added doenjangs caused 70% $\sim$ 77% inhibition on the proliferation of PC-3 human prostate cancer cells at 0.5 mg/mL while the control doenjang exhibited 46% inhibition. However, 2% GTE added doenjang showed 91% inhibition at 1.0 mg/mL. The trend of the inhibition rate was similar in DU14S human prostate adenocarcinoma cells. When bamboo salt was used instead of natural sea salt, the antimutagenicity against MNNG and in vitro anticancer effect on the prostate cancer cells greatly increased. From these results, it can be concluded that green tea extract addition to doenjang and the use of bamboo salt during doengjang preparation increased the antimutagenic and in vitro anticancer activities of the doenjang and showed a synergistic effect.

References

  1. Park KY, Jung KO. 2005. Fermented soybean products as functional Foods: Functional properties of doenjang (fermented soybean paste). In Asian Functional foods. Shi J, Ho CT, Shahidi F, eds. CRC Press, Boca Raton, FL. p 555-596
  2. Lee SJ. 2002. Studies on the enhancement of cancer preventive effects of doenjang. MS Thesis. Pusan National University
  3. Kim SJ, Moon JS, Park IB, Kim JM. 2004. The quality of soybean paste (Doenjang) prepared with sea tangle, sea mustard, and anchovy powder. J Korean Soc Food Sci Nutr 33: 875-879 https://doi.org/10.3746/jkfn.2004.33.5.875
  4. Ha JO, Park KY. 1998. Comparison of mineral content and external structure of various salts. J Korean Soc Food Nutr 27: 413-418
  5. Hwang KM. 2004. Studies on the enhancement of chemopreventive and anticancer effects of doenjang. PhD Dissertation. Pusan National University
  6. Kim SO, Park SY, Rhee SH, Park KY. 2003. Increased functional properties of chungkukjang prepared with bamboo salt. J Kor Assoc Cancer Prev 8: 286-296
  7. Chung HY, Yokozawa T. 1995. Studies on antiaging and antimutagenic mechanism of epigallocatechin 3-O-gallate isolated from green tea. Food Science Industry 28: 46-58
  8. Cheng J. 1992. The effects of Chinese tea on the occurrence of esophageal tumors induced by N-nitrosomethylbenzylamine in rats. Prev Med 21: 385-391 https://doi.org/10.1016/0091-7435(92)90046-K
  9. Yang F, Villiers WJ, Varilek GW. 1998. Green tea polyphenols block endotoxin-induced tumor necrosis factor-production and lethality in a murine model. J Nutr 128: 2334-2341
  10. Khan SG, Katitar SK, Agarwal R, Mukhtar H. 1992. Enhancement of antioxidant and phase II enzymes by oral feeding of green tea polyphenols in drinking water to SKH-1 hairless mice: possible role in cancer chemoprevention. Cancer Res 52: 4050-4052
  11. Machlin LJ, Bendich A. 1987. Free radical tissues damage. Protective role of antioxidant nutrients. FASEB J 1: 441- 445 https://doi.org/10.1096/fasebj.1.6.3315807
  12. Dulloo AG, Seydoux J, Girardier L, Chantre P, Vandermander J. 2000. Green tea and thermogenesis: interactions between catechin-polyphenols, caffeine and sympathetic activity. Int J Obesity 24: 252-258 https://doi.org/10.1038/sj.ijo.0801101
  13. Park JH, Ha AW, Cho JS. 2005. Effects of green tea-soybean paste on weights and serum lipid profiles in rats fed high fat diet. Korean J Food Sci Technol 37: 806-81
  14. Jung BM, Roh SB. 2004. Physicochemical quality comparison of commercial doenjang and traditional green tea doenjang. J Korean Soc Food Sci Nutr 33: 132-13 https://doi.org/10.3746/jkfn.2004.33.1.132
  15. Kong CS, Kim SO, Kil JH, Rhee SH, Han MS, Park KY. 2005. Increased anticancer effect of kochujang (Korean red pepper soybean paste) added with green tea extract and catechin. Cancer Prev Res 10: 206-210
  16. Kim JH, Kim SI, Kim JG, Im DK, Park JG, Lee JW, Byun MW. 2006. Effect of green tea powder on the improvement of sensorial quality of chungkookjang. J Korean Soc Food Sci Nutr 35: 482-486 https://doi.org/10.3746/jkfn.2006.35.4.482
  17. Setchell KDR, Cassidy A. 1999. Dietary isoflavones: biological effects and relevance to human health. J Nutr 129: 758-767 https://doi.org/10.1093/jn/129.3.758S
  18. Ames BN, McCann J, Yamasaki E. 1975. Methods for detecting carcinogens and mutagens with the Salmonella mammalian-microsome mutagenicity test. Mutat Res 31: 347-364 https://doi.org/10.1016/0165-1161(75)90046-1
  19. Ames BN, Profet M, Gold LS. 1990. Nature's chemicals and synthetic chemicals: comparative toxicology. Proc Natl Acad Sci USA 87: 7782-7786 https://doi.org/10.1073/pnas.87.19.7782
  20. Ames BN, Kammen HO, Yamasaki E. 1975. Hair dye are mutagenic: identification of variety of mutagenic ingredients. Proc Natl Acad Sci USA 72: 2423-2427 https://doi.org/10.1073/pnas.72.6.2423
  21. Skehan P, Storeng R, Monks SA, McMahon J, Vsitica D, Warren JT, Bokesch J, Kenney S, Boyd KR. 1990. New colorimetric cytotoxicity assay for anticancer- drug screening. J Natl Cancer Inst 82: 1107-1112 https://doi.org/10.1093/jnci/82.13.1107
  22. Kang WS, Lee YH, Chung HH, Kang MK, Kim TJ, Hong JT, Yun YP. 2001. Effects of green tea catechins on the lipid peroxidation and superoxide dismutase. J Food Hyg Safety 16: 41-47
  23. Muramatsu K, Fukuyo M, Hara Y. 1986. Effect of green tea catechins on plasma cholesterol level in cholesterol fed rats. J Nutr Sci Vitaminol 32: 613-622 https://doi.org/10.3177/jnsv.32.613
  24. Kim MJ, Rhee SJ. 1994. Effects of Korean green tea, oolong tea and black tea beverage on the removal of cadmium in rat. J Korean Soc Food Nutr 23: 784-791
  25. Sakanaka S, Shimura N, Aizawa M, Kim M, Yamato T. 1992. Preventive effect of green tea polyphenols against dental caries in conventional rats. Biosci Biotech Biochem 56: 592-594 https://doi.org/10.1271/bbb.56.592
  26. Wang ZY, Hong JY, Huang MT, Reuhl KR, Conney AH, Yang CS. 1992. Inhibition of N-nitrosodiethylamine- and 4-(methylnitorsamino)-1(3-pyridyl)-1-butanone-induced tumorigenesis in A/J mice by green tea and black tea. Cancer Res 52: 1943-1947
  27. Kada T, Kaneko K, Matsuzaki T, Hara Y. 1985. Detection and chemical identification of natural bioantimutagens. Mutat Res 150: 127-131 https://doi.org/10.1016/0027-5107(85)90109-5
  28. Faas MM, Van Der Schaaf G, Schipper M, Moes H. 2003. Glomerular immunoglobulin deposits induce glomerular inflation in pregnant but not in non-pregnant rats. J Reprod Immunol 49: 57-63 https://doi.org/10.1034/j.1600-0897.2003.00035.x
  29. Matsuzaki T, Hata Y. 1985. Antioxidative activity of tea leaf catechins. Nippon Nogeikagaku Kaish 59: 129-134 https://doi.org/10.1271/nogeikagaku1924.59.129
  30. Kzuko N, Midori Y, Chikusa T, Michiko I, Mitsuo N. 1991. Platelet aggregation inhibitory activity of tea extracts. Nippon Shokuhin Kogyo 38: 189-195 https://doi.org/10.3136/nskkk1962.38.189
  31. Yeo SG, Kim IS, Ahn CW, Kim SB, Park YH. 1995. Desmutagenicity of tea extracts from green tea, oolong tea and black tea. J Korean Soc Food Sci Nutr 24: 160-168
  32. Oguni I, Chen SJ, Lin PZ, Hara Y. 1992. Protection against cancer risk by Japanese green tea. Prev Med 21: 332
  33. Han C, Xu Y. 1990. The effect of Chinese tea on the occurrence of esophageal tumor induced by N-nitrosomethylbenzylamine in rats. Biomed Environ Sci 3: 35-42
  34. Wang ZY, Agarwal R, Khan WA, Mukhtar H. 1992. Protection against benzo(a)pyrene and N-ntrosodiethyl-amine-induced lung and forestomach tumorigenesis in A/J mice by water extracts of green tea and licorice. Carcinogenesis 13: 1491-1494 https://doi.org/10.1093/carcin/13.8.1491
  35. Yamane T, Takahashi T, Kuwata K, Oya K, Inagake M, Kitao Y, Suganuma M, Fujiki H. 1995. Inhibition of N-methyl-N'-nitro-N-nitrosoguanidine-induced carcinogenesis by (-)-epigallocatechin gallate in the rat glandular stomach. Cancer Res 55: 2081-2084

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