Antioxidant Activities and Xanthine Oxidase Inhibitory Effects of Hot-water Extracts from Fruits of Elaeagnus multiflora Thunb. in Maturity

성숙에 따른 뜰보리수(Elaeagnus multiflora Thunb.) 열수추출물의 항산화 활성과 Xanthine Oxidase 저해효과

  • Yoon, Kyung-Young (Dept. of Food and Nutrition, Yeungnam University) ;
  • Hong, Ju-Yeon (Faculty of Herbal Cuisine and Nutrition, Deagu Hanny University) ;
  • Nam, Hak-Sik (Faculty of Herbal Cuisine and Nutrition, Deagu Hanny University) ;
  • Moon, Yong-Sun (Dept. of Horticulture, Yeungnam University) ;
  • Shin, Seung-Ryeul (Faculty of Herbal Cuisine and Nutrition, Deagu Hanny University)
  • 윤경영 (영남대학교 식품영양학과) ;
  • 홍주연 (대구한의대학교 한방식품조리영양학부) ;
  • 남학식 (대구한의대학교 한방식품조리영양학부) ;
  • 문용선 (영남대학교 원예학과) ;
  • 신승렬 (대구한의대학교 한방식품조리영양학부)
  • Published : 2007.01.31


This study was conducted to analyze the antioxidant activity and xanthine oxidase inhibitory effect of hot-water extracts from Elaeagnus multiflora Thunb. to provide fundamental data for the development of functional materials. The antioxidative activities of hot-water extracts from E. multiflora Thunb. were analyzed by electron donating ability (EDA) using 1,1-diphenyl-2-picryl hydrazyl (DPPH), superoxide dismutase (SOD) -like activity by pyrogallol and nitrite scavenging ability. EDA of extracts from unripe, ripe, and overripe fruits were 77.7%, 36.0%, and 23.7% at 100 ${\mu}g/mL$, respectively, and EDA of extract in unripe fruits was higher than those of others. At 1,000 $\mu$g/mL, the SOD-like activities were 32.8%, 11.2% and 5.0% for unripe, ripe, and overripe fruits, respectively. The SOD-like activity was increased along with the increase of unripe and ripe extract concentrations. The nitrite scavenging ability of unripe fruit extracts in 100, 300, 500 ${\mu}g/mL$ of extraction solution in pH 1.2 and 3.0 was higher than those of others. The nitrate scavenging ability of all extracts was decreased according to the increase of pH. Xanthine oxidase inhibitory activities of unripe, ripe and overripe fruits were 30.0%, 28.2%, and 18.2% at 1,000 ${\mu}g/mL$, respectively.


  1. Ames BN. 1970. Identification of environmental chemicals causing mutation and cancer. Science 204: 589-592
  2. Hammond B, Kontos A, Hess ML. 1985. Oxygen radicals in the adult respiratory distress syndrome, in myocardial ischemia and reperfusion injury, and in cerebral vascular damage. Can J Physion Pharmacol 63: 173-187
  3. Shin DH. 1997. The study course and movement of natural antioxidants. Food Sci Industry 30: 14-21
  4. Pratt DE. 1992. Natural antioxidants from plant materials. In Phenolic compounds in food and their effects on health (II). Huang MT, Ho ST, Lee CY, eds. Am Chem Soc, Washington DC. p 54-60
  5. Ames BN, Cahcart R, Schwiers E, Hochstein P. 1981. Uric acid provides an antioxidant defense in humans against oxidant and radical-caused aging and cancer. Proc Natl Acad Sci USA 78: 6858-6862
  6. Tsuda T, Watanabw M, Ohshima K, Norinobu S, Choi SW, Kawakishi S, Osawa T. 1994. Antioxidative activity of the anthocyanin pigments cyanidin 3-O-${\beta}$-D-glucoside and cyanidin. J Agric Food Chem 42: 2407-2410
  7. Brannen AL. 1975. Toxicology and biochemistry of butyrated hydroxy toluene and bytyrated hydroxy anisole. J Am Oil Chem Soc 52: 59-63
  8. Halliwell B, Hoult RJ, Blake DR. 1988. Oxidants, inflammation, and anti-imflammatory drugs. FASEB J 2: 2867-2870
  9. Hwang JY, Ham JW, Nam SH. 2004. The antioxidant activity of Maesil (Prunus mume). Korean J Food Sci Technol 36: 461-464
  10. Cha WS, Shin HR, Park JH, Oh SL, Lee WY, Chun SS, Choo JW, Cho YJ. 2004. Antioxidant activity of phenol compounds from mulberry fruits. Korean J Food Preserv 11: 383-387
  11. Moon JS, KimSJ, Park YM, Hwang IS, Kim EH, Park JW, Park IB, Kim SW, Kang SG, Park YK, Jung ST. 2004. Activities of antioxidation and alcohol dehydrogenase inhibition of methanol extracts from some medicinal herbs. Korean J Food Preserv 11: 201-206
  12. Yu MH, Im G, Lee HJ, Ji YJ, Lee IS. 2006. Components and their antioxidative activities of methanol extracts from sarcocarp and seed of Zizyphus jujuba var. inermis Rehder. Korean J Food Sci Technol 38: 128-134
  13. Othman A, Ismail A, Ghani NA, Adenan I. 2007. Antioxidant capacity and phenolic content of cocoa beans. Food Chem 100: 1523-1530
  14. Kuda T, Hishi T, Maekawa S. 2006. Antioxidant properties of dried product of 'haba-nori', edible brown alga, Petalonia binghamiae (J. Agaradh) Vinogradova. Food Chem 98: 545-550
  15. Sakanaka S, Tachibana Y, Okada Y. 2005. Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha). Food Chem 89: 569-575
  16. Rao LJM, Borse RBB, Raghavan B. 2007. Antioxidant and radical-scavenging carbazole alkaloids from the oleoresin of curry leaf (Murraya koenigii Spreng.). Food Chem 100: 742-747
  17. 조무생. 1989. 원색한국수목도감. 아카데미, 서울. p 372
  18. 고경식. 1991. 한국식물검색도감. 아카데미, 서울. p 224
  19. 이창복. 1980. 대한식물도감. 향문사, 서울. p 198
  20. Kim NW, Joo EY, Kim SL. 2003. Analysis on the components of the fruit of Elaeagnus muliflora Thunb. Korean J Food Preserv 10: 534-539
  21. Hong JY, Nam HS, Lee YS, Yoon KY, Kim NW, Shin SR. 2006. Study on the antioxidant of extracts from the fruit of Elaeagnus muliflora Thunb. Korean J Food Preserv 13: 413-419
  22. Blois ML. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1224
  23. Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474
  24. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibitory of nitrosamine formation by nondilyzable melanoidins. Agric Biol Chem 51: 1333-1338
  25. Stirpe F, Della Corte E. 1969. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). J Biol Chem 244: 3855-3863
  26. Lee YS, Joo EY, Kim NW. 2005. Antioxidant activity of extracts from the Lespedeza bicolor. Korean J Food Preserv 12: 75-79
  27. Kang YH, Park YK, Lee GD. 1996. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 28: 232-239
  28. Tsai SY, Huang SJ, Mau JL. 2006. Antioxidant properties of hot-water extracts from Agrocybe cylindracea. Food Chem 98: 670-677
  29. Abdille MdH, Singh RP, Jayaprakasha GK, Jena BS. 2005. Antioxidant activity of the extracts from Dillenia indica fruits. Food Chem 90: 891-89
  30. 한대석, 김석중. 1994. SOD유사활성물질과 기능성식품의 개발. 식품기술 7: 41-49
  31. Shin SR, Hong JY, Nam HS, Yoon KY, Kim KW. 2006. Anti-oxidative effects of extracts of Korean herbal materials. J Korean Soc Food Sci Nutr 35: 187-191
  32. Peter FS. 1975. The toxicology of nitrate, nitrite and N-nitrocompounds. J Sci Food Agric 26: 1761-1769
  33. Fiddler W, Pensabene JW, Piotrowski EG, Doerr RC, Wasserman AE. 1973. Use of sodium ascorbate or erythrobate to inhibit formation of N-nitrosodimethylamine in frankurters. J Food Sci 38: 1084-1091
  34. Chiang HC, Lo YJ, Lu Fj. 1994. Xanthine oxidaseinhibitions from leaves of Alsophila spinulose (HOOK) Tryon. J Enzyme Inhibitions 8: 61-71
  35. Ferraz Filha ZS, Vitolo IF, Fietto LG, Lombardi JA, Saude-Guimaraes DA. 2006. Xanthine oxidase inhibitory activity of Lychnophora species from Brazil ('Arnica'). J Ethnophamacology 107: 79-82
  36. Moon SH, Lee MK, Chae KS. 2001. Inhibitory effects of the solvent fraction from persimmon leaves on xanthine oxidase activity. Korean J Food Nutr 14: 120-125
  37. Ra KS, Chung SH, Suh HJ, Son JY, Lee HK. 1998. Inhibitor of xanthine oxidase from onion skin. Korean J Food Sci Technol 30: 697-701
  38. An BJ, Bae MJ, Choi C. 1996. Inhibitory effects of flavan-3-ols isolated from Oolong tea on xanthine oxidase. Korean J Food Sci Technol 28: 1084-1088

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