Antioxidative Activity and Physiological Function of the Angelica dahurica Roots

백지의 항산화성 및 생리기능

  • 이양숙 (대구한의대학교 한방생약자원학과) ;
  • 장상민 (남성고등학교) ;
  • 김남우 (대구한의대학교 한방생약자원학과)
  • Published : 2007.01.31


We analyzed the contents of polyphenol compounds, the antioxidant activity and the physiological activity to investigate the functional effects of extracts from Angelica dahurica by the reflux water extraction (RW), reflux ethanol extraction (RE) and pressure heating water extraction (PW). The content of phenolic compounds of PW was the highest at 156.30 mg/g, and those of RW and RE were 31.69 mg/g and 26.34 mg/g, respectively. The electron donating ability (EDA) were in the range of 30.56% $\sim$ 52.74% and superoxide dismutase (SOD) like activity were 10.96% $\sim$ 23.24% at 1,000 ${\mu}g/mL$. The nitrite scavenging ability of PW at pH 1.2 was 61.47%, higher than those of RW (16.81%) and RE (17.78%). The xanthine oxidase inhibitory were 90.91% and tyrosinase inhibitory rate of RE was the highest (51.71%) at the concentration of 5,000 ${\mu}g/mL$. All extracts were increased with increments of the extract concentrations.


  1. Hammond B, Kontos HA, Hess ML. 1985. Oxygen radicals in the adult respiratory distress syndrome, in myocardial ischemia and reperfusion injury, and in cerebral vascular damage. Can J Physiol Pharmacol 63: 173-187
  2. McCord JM. 1987. Oxygen-derived radicals; a link between repercussion injury and inflammation. Fed Proc 46: 2402- 2406
  3. Gardner DR, Fridovich I. 1991. Superoxide sensitivity of Escherichia coli 6-phosphogluconate dehydratase. J Biol Chem 266: 1478-1783
  4. Labuza TP. 1971. Kinetic of lipid oxidation in foods. CRC Crit Rev Food Technol 2: 355-405
  5. Frankel EN, Waterhouse Al, Kinsella JE. 1993. Inhibition of human LDL oxidation by resveratrol. Lancet 341: 1103- 1104
  6. Rice-Evans CA, Miller HJ, Oaganga G. 1996. Structure antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 20: 933-956
  7. Azuma K, Nakayama M, Koshika M, Lpoushi K, Yamaguchi Y, Kohata K, Yamaguchi Y, Ito H, Higashio H. 1999. Phenolic antioxidants from the leaves of Corchorus olitorius L. J Agric Food Chem 47: 3963-3966
  8. Rein D, Paglieroni TG, Wun T, Oearson DA, Schmitz HH, Gosselin R, Keen CL. 2000. Cocoa inhibits platelet activation and function. Am J Clin Nutr 72: 30-35
  9. Chan KM, Decher EA, Means WJ. 1993. Extraction and activity of carmosine, a naturally occurring antioxidant in beef muscle. J Food Sci 58: 1-4
  10. Hatano T. 1995. Constituents of natural medicines with scavenging effect on active oxygen species-tannins and related polyphenols. Natural Medicine 49: 357-363
  11. Tsuda T, Watanabe M, Ohshima K, Norinobu S, Choi SW, Kawakishi S, Osawa T. 1994. Antioxidative activity of the anthocyanin pigments cyanidin 3-O-${\beta}$-D-glucoside and cyanidin. J Agic Food Chem 42: 2407-2410
  12. Akaike T, Ijiri S, Sato J, Katsiki T, Maeda H. 1995. Determination of peroxyl radical-scavenging activity in food by using bactericidal action of alkyl peroxyl radical. J Agric Food Chem 43: 1864-1870
  13. Kim HS, Choi HJ. 1990. Studies on essential oils of plants of Angelica genus in Korea (III). Kor J Pharmacogn 21: 121-125
  14. 구본홍. 1994. 동의보감 한글완역본(허준 저). 대중서관, 서울
  15. Kimura T, But PPH, Guo JX, Sung CK. 1996. International collation of traditional and folk medicine: Part 1. World scientific, Singapore. p 117-118
  16. Joo EY, Kang WJ. 2005. Analysis on the components of the Angelica dahurica root. Korean J Food Preserv 12: 476-481
  17. Kim SH, Kang SS, Kim CM. 1992. Coumarin glycosides from the roots of Angelica dahurica. Arch Pharm Res 15: 73-77
  18. Kwon YS, Kim CM. 1992. Coumarin glycosides from the roots of Angelica dahurica. Kor J Pharmacogn 24: 221-224
  19. Baek NI, Ahn EM, Kim HY, Park YD. 2000. Furanocoumarins from the root of Angelica dahurica. Arch Charm Res 23: 467-470
  20. Ryu SY, Kim JC, Kim YS, Kim HT, Kim WK, Choi GJ, Kim JS, Lee SW, Heor JH, Cho KY. 2001. Antifungal activities of coumarins isolated from Angelica gigas and Angelica dahurica against plant pathogenic fungi. Korean J Pesticide Science 5: 26-35
  21. Wang NH, Yoshiazaki K, Baba K. 2001. Seven new bifuranocoumarins, dahuribirin A-G, from Japanese Bai Zh. Chem Pharm Bull 49: 1085-1088
  22. Jin MH, Jung MH, Lim YH, Lee SH, Kang SJ, Cho WG. 2004. Promoting synthesis of collagen from Angelica dahurica root. Kor J Pharmacogn 35: 315-319
  23. Shin KH, Kim ON, Woo WS. 1988. Effect of the constituents of Angelica dahurica Radix on hepatic drug metabolizing enzyme activity. Kor J Pharmacogn 19: 19-27
  24. Kim DK, Lim JP, Yang JH, Eom DO, Eun JS, Leem KH. 2002. Acetylcholinesterase inhibitors from the roots of Angelica dahurica. Arch Pharm Res 25: 856-859
  25. Kim CM, Kwon YS, Choi SY. 1995. Antithrombotic effect of the BuOH soluble fraction of Angelica dahurica root. Kor J Pharmacogn 26: 74-77
  26. Pae HO, Oh HC, Yun YG, Oh GS, Jang SI, Hwang KM, Kwon TO, Lee HS, Chung HT. 2002. Imperatorin, a furanocoumarin from Angelica dahurica (Umbelliferae), induces cytochrome c-dependent apoptosis in human promyelocytic leukaemia, HL-60 cells. Pharmacol Toxicol 91: 40-48
  27. Schinella GR, Tournier HA, Prieto JM, Rios JL, Buschiazzo H, Zaidenberg A. 2002. Inhibition of Trypanosoma cruzi growth by medical plant extracts. Fitoterapia 73: 569-575
  28. Lechner D, Stavri M, Oluwatuyi M, Pereda-Miranda R, Gibbons S. 2004. The anti-staphylococcal activity of Angelica dahurica. Phytochemistry 65: 331-335
  29. Kwon YS, Kobayashi A, Kajiyama SI, Kawazu K, Kanzaki H, Kim CM. 1997. Antimicrobical constituents of Angelica dahurica roots. Phytochemistry 44: 887-889
  30. Swain T, Hillis WE, Ortega M. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. J Sci Food Agric 10: 83-88
  31. AOAC. 2005. Official Method of Analysis. 18th ed. Association of official analytical chemists, Washington DC, USA. Vol 45, p 21-22
  32. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200
  33. Marklund S, Marklund G. 1975. Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 468-474
  34. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric Biol Chem 51: 1333-1338
  35. Stirpe F, Corte ED. 1969. The regulation of rat liver xanthine oxidase. J Biol Chem 244: 3855-3861
  36. Yagi A, Kanbara T, Morinobu N. 1986. The effect of tyrosinase inhibition for aloe. Planta Medica 3981: 517-519
  37. Cha JY, Kim HJ, Chung CH, Cho YS. 1999. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J Korean Soc Food Sci Nutr 28: 1310-1315
  38. Lee JH, Lee SR. 1994. Analysis of phenolic substances content on Korea plant foods. Korean J Food Sci Technol 26: 310-316
  39. Moon JS, Kim SJ, Park YM. 2004. Activities of antioxidation and alcohol dehydrogenase inhibition of methanol extracts from some medicinal herbs. Korean J Food Preserv 11: 201-206
  40. Kang MJ, Shin SR, Kim K. 2002. Antioxidative and free radical scavenging activity of water extract from dandelion (Taraxacum officinale). Korean J Food Preserv 9: 253-259
  41. Kim HK, Kim YE, Do JR, Lee YC, Lee BY. 1995. Antioxidative activity and physiological activity of some Korean medical plants. Korean J Food Sci Technol 27: 80-85
  42. Lee YS, Joo EY, Kim NW. 2005. Antioxidant activity of extracts from the Lespedeza bicolor. Korean J Food Preserv 12: 75-79
  43. Jung SH, Jo WA, Son JH, Choi EY, Park CI, Lee IC, An BJ, Son AR, Son AR, Kim SK, Kim YS, Lee JT. 2005. A study on the application of cosmetic materials and the physiological activities of Forsythia koreana Nakai. Kor J Herbology 20: 61-68
  44. An BJ, Lee JT. 2002. Studies on biological activity from extract of Crataegi fructus. Kor J Herbology 17: 29-38
  45. Lim JD, Yu CY, Kim MJ, Yun SJ, Lee SJ, Kim NY, Chung IM. 2004. Comparison of SOD activity and phenolic compound contents in various Korean medicinal plants. Korean J Medicinal Crop Sci 12: 191-202
  46. Kim SM, Cho YS, Sung SL. 2001. The antioxidant ability and nitrate scavenging ability of plant extract. Korean J Food Sci Technol 33: 623-632
  47. Park YB, Lee TG, Kim OK, Do JR, Yeo SG, Park YH, Kim SB. 1995. Characteristics of nitrite scavenger derived from seeds of Cassia tora L. Korean J Food Sci Technol 27: 124-128
  48. Chung SY, Kim MK, Yoon S. 1999. Nitrite scavenging effect of method fraction obtained from green yellow vegetable juices. Korean J Food Sci Technol 28: 342-347
  49. Wyngaarden JB, Holmes EW Jr. 1977. Molecular nature of enzyme regulation in purine biosynthesis. Ciba Found Symp 48: 43-64
  50. Storch I, Ferber E. 1988. Detergent-amplified chemiluminescence of lucigenin for determination of superoxide amino production by NADPH oxidase and xanthine oxidase. Anal Biochem 169: 262-267
  51. Hatano T, Yasuhara T, Yoshihara R, Okuda T. 1991. Inhibitory effects of galloylated flavonoids on xanthine oxidase. Planta Medica 57: 83-86
  52. Moon SH, Lee MK. 1998. Inhibitory effects of xanthine oxidase by boiled water extract and tannin from persimmon leaves. Korean J Food Nutr 11: 354-357
  53. Yeo SG, Park YB, Kim IS, Kim SB, Park YH. 1995. Inhibition of xanthine oxidase by tea extracts from green tea, oolong tea and black tea. J Korea Soc Food Sci Nutr 24: 154-159
  54. Choi BW, Kee BH, Kang JH. 1998. Screening of the tyrosinase inhibitors from marine algae and medicinal plants. Kor J Pharmacogn 29: 237-242
  55. Jung SW, Lee NK, Kim SJ, Han DW. 1995. Screening of tyrosinase inhibitor from plants. Korean J Food Sci Technol 27: 891-896

Cited by

  1. Antioxidant Activities of Solvent Extracts from Pomegranate Endocarp vol.40, pp.12, 2011,
  2. Inhibitory Effects of Angelicae Dahuricae Radix Extract on COPD induced by Cigarette Smoke Condensate and Lipopolysaccharide in Mice vol.19, pp.5, 2011,
  3. Anti-oxidative Activities of Angelica dahurica Radix Ethanol Extract vol.12, pp.10, 2011,
  4. Whitening Effects of Angelica dahurica Radix Ethanol Extract vol.12, pp.9, 2011,
  5. Antioxidant, Anti-Inflammatory and Antiproliferative Activity ofAngelica DahuricaRoot Extracts vol.38, pp.3, 2014,
  6. Antioxidant Activities of Bamboo (Sasa Borealis) Leaf Extract according to Extraction Solvent vol.38, pp.12, 2009,