DOI QR코드

DOI QR Code

Effects of Mulberry Leaf Extract Feeding on Lipid Status of Rats Fed High Cholesterol Diets

뽕잎추출물이 고콜레스테롤 식이 흰쥐의 지질대사에 미치는 영향

  • Park, Surk-Hoon (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Jang, Mi-Jin (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Hong, Jung-Hee (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Rhee, Soon-Jae (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Choi, Kyung-Ho (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Park, Mo-Ra (Dept. of Food Science and Nutrition, Sanju National University)
  • 박석훈 (대구가톨릭대학교 식품영양학과) ;
  • 장미진 (대구가톨릭대학교 식품영양학과) ;
  • 홍정희 (대구가톨릭대학교 식품영양학과) ;
  • 이순재 (대구가톨릭대학교 식품영양학과) ;
  • 최경호 (대구가톨릭대학교 식품영양학과) ;
  • 박모라 (국립상주대학교 식품영양학과)
  • Published : 2007.01.31

Abstract

This study was to investigate the effect of mulberry leaf water extract on lipid metabolism of rats fed high cholesterol diets. Sprague-Dawley male rats were randomly assigned to two normal groups; mulberry extract-free (N), 0.16% mulberry leaf extract (NM) groups and high cholesterol groups with four different levels of mulberry leaf extract; 0% (HC), 0.08% (HCL), 0.16% (HCM), and 0.32% mulberry leaf extract (HCH) groups. Serum levels of triglyceride, cholesterol, LDL-cholesterol and AI index in mulberry leaf extract supplemented groups were significantly lower than the HC group (p<0.05). The level of HDL-cholesterol in the HC group was significantly (p<0.05) reduced, compared with N group, but it was increased by mulberry leaf extract supplementation. Mulberry leaf extract had no effect on the UDP-glucuronyl transferase activity. Also, there was no significant difference in the level of liver cholesterol between the HC and mulberry leaf extract supplemented groups, while there was significant difference in the levels of liver total lipid and triglyceride. The HCM and HCH groups had more significant reduction in the activity of lipoprotein lipase in epididymal fat tissue than the HC group. The levels of total lipid, triglyceride and total cholesterol in epididymal fat tissue of HCL, HCM and HCH groups were decreased compared to HC group. The levels of total lipid, triglyceride and total cholesterol in feces from HCL, HCM and HCH groups were higher than those of HC group.

References

  1. 대한통계협회. 2004. 사망원인 통계 연보
  2. Kohomoto T, Fukui F, Takaku H, Machida Y, Mitsuoka T. 1998. Effect of isomaltooligosaccharide on human fecal flora. Biofidobacteria Microflora 7: 61-67
  3. Kim AJ, Yuh CS, Bang IS, Woo KJ. 2006. Study on preparation and quality of jelly using mulberry leaf powder. Kor J Food Cookery Sci 22: 56-61
  4. Kim HB, Kim AJ, Kim SY. 2003. The analysis of functional materials in mulberry fruit and feed product development trends. Food Sci Industry 36: 49-60
  5. Naitoh K. 1968. Studies on the micro constituent in mulberry leaves part 2. Isolation of rutin and quercetin from mulberry leaves. Nippon Nogei Kagaku Kaishi 42: 422-425
  6. Shin KH, Young HS, Lee TW, Choi JS. 1995. Studies on the chemical component and antioxidative effects of Solanum lyratum. Kor J Pharmacogn 26: 130-138
  7. Kim JS, Kang SS, Lee MW, Kim OK. 1995. Isolation of flavonoids from the leaves of Aralia continentails. Kor J Phamacogn 26: 239-243
  8. Onogi A, Osawa K, Yasuda H, Sakai A, Morita H, Tokawa H. 1993. Flavonol glycosides from the leaves of Morus alba. Shoyakugaku Zasshi 47: 423-425
  9. Kang SS, Woo WS. 1984. Flavonol glycosides from the leaves of Zizyphus jujuba. Kor J Pharmacogn 15: 170-178
  10. Kim JS, Kang SS, Lee MW, Kim OK. 1995. Isolation of flavonoids from the leaves of Aralia continentalis. Kor J Pharmacogn 26: 239-243
  11. Kodama T, Ishida H, Kokubo T, Yamakawa T, Noguchi H. 1990. Glucosylation of quercetin by a cell suspension culture of vitis species. Agric Biol Chem 54: 3238-3288
  12. Do JC, Yu YJ, Jung KY, Son KH. 1992. Flavonoids from the leaves of Polygala japonica. Kor J Pharmacogn 23: 9-13
  13. Yoshikumi Y. 1994. Inhibition of intestinal $\alpha$-glycosidase activity and postprandial hyperglycemia by moranoline and its N-alkylderivatives. Agric Biol Chem 52: 121-126
  14. Asano N, Tomioka E, Kizu H, Matsui K. 1994. Sugars with nitrogen in the ring isolated from the leaves of Morus bombycis. Carbohydr Res 253: 235-245 https://doi.org/10.1016/0008-6215(94)80068-5
  15. Yagi M, Kouno T, Acyagi Y, Murai H. 1976. The structure of morano line, a piperidine alkaloid from morus species. Nippon Nogei Kagaku 50: 571-572 https://doi.org/10.1271/nogeikagaku1924.50.11_571
  16. Basnet P, Kadota S, Terashima S, Simazu M, Namba T. 1993. Two new 2-arylbenzofuran derivatives from hypoglycemic activity-bearing fractions of Morus insignis. Chem Pharm Bull 41: 1238-1243 https://doi.org/10.1248/cpb.41.1238
  17. Kimura M, Chen F, Nakashimqa N, Kimura I, Asano N, Koya S. 1995. Anti hyperglycemic effect of N-containing sugars delived from mulberry leaves in streptozotocin-induced diabetic mice. J Traditional Medicine 12: 214-219
  18. Sung GB. 1998. Recent mulberry research trend and direction for the improvement. Korean J Seric Sci 40: 180-184
  19. Yen GC, Wu SC, Duh PD. 1996. Extraction and identification of anti-oxidant components from the leaves of mulberry (Morus alba L.). J Biol Chem 261: 12879-12882
  20. Fujimoto T, Nomura T. 1985. Components of root bark of cudrania tricuspidata 3. Isolation and structure studies on the flavonoids. Planta Med 51: 190-196 https://doi.org/10.1055/s-2007-969453
  21. Kim SH, Kim NJ, Choi JS, Park JC. 1993. Determination of flavonoids by HPLC and biological activities from the leaves of Cudrania tricuspidata Bureau. J Korean Soc Food Nutr 22: 68-72
  22. Chen F, Nakashima N, Kimura I, Kimura M, Asano N, Koya S. 1995. Potentiating effects on pilocarpine-induced saliva secretion by extracts and N-containing sugars derived from mulberry leaves in streptozotocin-diabetic mice. Biol Pharm Bull 18: 1676-1680 https://doi.org/10.1248/bpb.18.1676
  23. Friedwaid WT, Levy RI, Fedreicson DS. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without was of the preparative ultracentrifuge. Clin Chem 18: 499-506
  24. Fiordaliso M, Kok N, Desager JP, Goethals F, Deboyser D, Roberfoid M, Delzenne N. 1977. Dietary oligofructose lowers triglycerides, phospholipids and cholesterol in serum and very low density lipoproteins of rats. Lipids 30: 163- 167 https://doi.org/10.1007/BF02538270
  25. Reinke LA, Moyer MJ, Notley KA. 1986. Diminished rates of glucuronidation and sulfation in perfused rat liver after chronic ethanol administration. Biochem Pharmacol 35: 439-447 https://doi.org/10.1016/0006-2952(86)90217-0
  26. Folch JM, Lees M, Stanley GHS. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem 26: 497-509
  27. Sale FD, Marchesini S, Fishman PH, Berra B. 1984. A sensitive enzymatic assay for determination of cholesterol in lipid extracts. Academic Press, New York. p 347-350
  28. Iverius PH, Ostlund-Lindqvist AM. 1986. Preparation, characterization and measurement of lipoprotein lipase. Methods Enzymol 129: 691-704 https://doi.org/10.1016/0076-6879(86)29099-0
  29. Parkin SM, Walker K, Ashby P, Robinson DS. 1980. Effects of glucose and insulin on the activation of lipoprotein lipase and on protein synthesis in rat adipose tissue. Biochem J 188: 193-199 https://doi.org/10.1042/bj1880193
  30. Riley SE, Robinson DS. 1974. Studies on the assay of clearing factor lipase. Biochem Biophys Acta 369: 371-387 https://doi.org/10.1016/0005-2760(74)90151-9
  31. Kim MS, Choue RW, Shung SH, Koo SJ. 1998. Blood glucose lowering effects of mulberry leaves and silkworm ex-tracts on mice fed with high-carbohydrate diet. J Kor Nutr 31: 117-125
  32. Kang JO, Kyoung SK. 1995. The effect of dry edible leaves feeding on serum lipids of hypercholesterolemic rats. J Korean Soc Food Nutr 24: 502-509
  33. Kim SY, Lee WC, Kim HB, Kim AJ, Kim SK. 1998. Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol-induced hyperlipidemia rats. J Korean Soc Food Sci Nutr 27: 1217-1222
  34. Doi K, Kojima T, Fujimoto Y. 2000. Mulberry leaf extract inhibits the oxidative modification of rabbit and human low density lipoprotein. Biol Pharm Bull 23: 1066-1071 https://doi.org/10.1248/bpb.23.1066
  35. Paul HA. 1979. Lipophilicity of acceptor substrate as a factor in late foetal rat liver microsomal UDP-glucuronosyltransferase activity. Biochem Pharmacol 29: 999-1006 https://doi.org/10.1016/0006-2952(80)90162-8
  36. Bandary S, Reddy BS. 1981. Diet and excretion of bile acids. Cancer Res 41: 3766-3768
  37. Semenkovich CF, Coleman T, Daugherty A. 1998. Effects of heterozygous lipoprotein lipase deficiency on diet-induced atherosclerosis in mice. J Lipid Res 39: 1141-1151
  38. Lopez-Soriano J, Llovera M, Carbo N, Garcia-Martinez C, Lopez-Soriano FJ, Argiles JM. 1997. Lipid metabolism in tumour-bearing mice: studies with knockout mice for tumour necrosis factor receptor 1 protein. Mol Cell Endocrinol 132: 93-99 https://doi.org/10.1016/S0303-7207(97)00125-1
  39. Carrozza G, Livrea G, Caponetti R, Manasseri L. 1979. Response of rat hepatic fatty acid synthesis and activities of related enzymes to changes in level of dietary fat. J Nutr 109: 162-170 https://doi.org/10.1093/jn/109.1.162
  40. Iritani N, Fukuda E, Inoguchi K. 1980. Reduction of lipogenic enzymes by shellfish triglycerides in rat liver. J Nutr 110: 1664-1670 https://doi.org/10.1093/jn/110.8.1664
  41. Toussant MJ, Wilson MD, Clarke SD. 1981. Coordinate suppression of liver acetyl-CoA carboxylase and fatty acid synthetase by polyunsaturated fat. J Nutr 111: 146-153 https://doi.org/10.1093/jn/111.1.146
  42. Cruz ML, Williamson DH. 1992. Refeeding meal-fed rats increased lipoprotein lipase activity and deposition of dietary $[^{14}C]$ lipid in white adipose tissue and decrease oxidation to $^{14}CO_{2}$. Biochem J 285: 773-778 https://doi.org/10.1042/bj2850773

Cited by

  1. Effects of Ramie Leaves on Improvement of Lipid Metabolism and Antiobesity Effect in Rats Fed a High Fat/High Cholesterol Diet vol.43, pp.1, 2011, https://doi.org/10.9721/KJFST.2011.43.1.083
  2. Anti-obesity Effects of Ishige okamurae Extract in C57BL/6J mice Fed High-fat Diet vol.45, pp.2, 2013, https://doi.org/10.9721/KJFST.2013.45.2.199
  3. Effects of Unripe Rubus coreanus Miquel Extract on Improvement of Lipid Metabolism in C57BL/6 Mice Fed a High-Cholesterol Diet vol.43, pp.5, 2014, https://doi.org/10.3746/jkfn.2014.43.5.650
  4. Effects of Garlic Shoot Extract on Lipid Metabolism in Hyperlipidemic Rats Fed a High-fat Diet vol.25, pp.3, 2015, https://doi.org/10.5352/JLS.2015.25.3.276
  5. Anti-inflammatory Effect of Lactuca sativa L. Extract in Human Umbilical Vein Endothelial Cells and Improvement of Lipid Levels in Mice Fed a High-fat Diet vol.29, pp.6, 2016, https://doi.org/10.9799/ksfan.2016.29.6.998
  6. Anti-Obesity Effect of Eriobotrya japonica Leaves Extract on Obese Mice Induced by High-Fat Diet vol.45, pp.8, 2016, https://doi.org/10.3746/jkfn.2016.45.8.1202
  7. Effect of Ledebouriella seseloides Extracts on Lipid Parameters in Ovariectomized Rats vol.26, pp.1, 2016, https://doi.org/10.5352/JLS.2016.26.1.83
  8. Combined Treatment of Mulberry Leaf and Fruit Extract Ameliorates Obesity-Related Inflammation and Oxidative Stress in High Fat Diet-Induced Obese Mice vol.16, pp.8, 2013, https://doi.org/10.1089/jmf.2012.2582
  9. Effect of combined mulberry leaf and fruit extract on liver and skin cholesterol transporters in high fat diet-induced obese mice vol.8, pp.1, 2014, https://doi.org/10.4162/nrp.2014.8.1.20
  10. Effect of Mulberry Extract on the Lipid Profile and Liver Function in Mice Fed a High Fat Diet vol.29, pp.3, 2016, https://doi.org/10.9799/ksfan.2016.29.3.411
  11. Effects of persimmon-vinegar on lipid and carnitine profiles in mice vol.19, pp.2, 2010, https://doi.org/10.1007/s10068-010-0049-3
  12. Effect of various sources of dietary additive on growth, body composition and serum chemistry of juvenile olive flounder (Paralichthys olivaceus) vol.46, pp.9, 2015, https://doi.org/10.1111/are.12388
  13. The Effects of Seaweed Gongjindan on Estrogen like Activities, Platelet Aggregation and Serum Lipid Levels in Ovariectomized Rats vol.23, pp.9, 2013, https://doi.org/10.5352/JLS.2013.23.9.1155
  14. Anti-obesity Effects of African Mango (Irvingia gabonesis, IGOB 131TM) Extract in Leptin-deficient Obese Mice vol.43, pp.10, 2014, https://doi.org/10.3746/jkfn.2014.43.10.1477
  15. Beneficial dietary effect of turmeric and sulphur on weight gain, fat deposition and lipid profile of serum and liver in rats vol.51, pp.4, 2014, https://doi.org/10.1007/s13197-011-0569-8
  16. Beneficial effect of Combination with Korean Red Ginseng, Gastrodia Rhizoma and Polygoni Multiflori on Cholesterol and Erectile Dysfunction in Hyperlipidemia rats vol.30, pp.6, 2015, https://doi.org/10.6116/kjh.2015.30.6.69.
  17. Mori Folium regulates DSS-induced ulcerative colitis in mice and cytokine production in mast cells vol.12, pp.2, 2012, https://doi.org/10.1007/s13596-012-0055-5
  18. Dietary Effects of Polymannuronate Added to Hamburger Buns on Lipid Metabolism in Rats vol.48, pp.2, 2015, https://doi.org/10.5657/KFAS.2015.0187
  19. Development of Mulberry-leaf Tea Containing γ-Aminobutyric Acid (GABA) by Anaerobic Treatments vol.47, pp.5, 2015, https://doi.org/10.9721/KJFST.2015.47.5.652
  20. Effect of Saekso 2 Corn Kernels and Cobs Extracts on Antioxidant Activity in Rats Fed High Fat-cholesterol Diet vol.31, pp.6, 2016, https://doi.org/10.13103/JFHS.2016.31.6.399