Anti-wrinkle Activity of Low Molecular Weight Peptides Derived from the Collagen Isolated from Asterias amurensis

불가사리(Asterias amurensis) 콜라겐 유래 저분자 펩타이드의 피부주름 억제활성

  • Kwon, Min-Chul (College of Biscience and Biotechnology, Kangwon National University) ;
  • Kim, Cheol-Hee (College of Biscience and Biotechnology, Kangwon National University) ;
  • Kim, Hyo-Sung (College of Biscience and Biotechnology, Kangwon National University) ;
  • Syed, Abdul Qadir (College of Biscience and Biotechnology, Kangwon National University) ;
  • Hwang, Bo-Yong (SKY007) ;
  • Lee, Hyeon-Yong (Research Institute of Bioscience and Biotechnology, Kangwon National University)
  • 권민철 (강원대학교 BT특성화학부대학) ;
  • 김철희 (강원대학교 BT특성화학부대학) ;
  • 김효성 (강원대학교 BT특성화학부대학) ;
  • ;
  • 황보영 ;
  • 이현용 (강원대학교 생명공학연구소)
  • Published : 2007.12.01


This study was carried out to investigate the anti-wrinkle effects of peptides derived from collagens isolated from Asterias amurensis, which was collected in the East Sea. The molecular weights of the peptides were between 10-50 kDa, as determined through sephadek G-75 gel. The cytotoxicities against CCD-986sk cells and HEL-299 cells were measured using the MTT assay. The cytotoxicity of all the fractions(F1: Fraction No. 4-13, 116 kDa; F2: Fraction No. 25-30, 100 kDa; F3: Fraction No. 45-55, 58 kDa; F4: Fraction No. 59-63, 43 kDa; F5: Fraction No. 79-90, 24 kDa) was less than 25%, by the addition of 1.0 mg/mL. These peptides did not show any adverse effects on human skin cells. In the presence of F1 at 1.0 mg/mL, matrix metalloproteinase-1 (MMP-1) expression of UVA-induced human normal fibroblasts was reduced to 34.8%. Overall, the results seem to suggest that peptides of approximately 20 kDa have superior anti-wrinkle effects.


Asterias amurensis;low molecular weight peptide;anti-wrinkle activity


  1. Lee CK, Lee DS, Hwang KC, Song KC, Jang YS. Component and effect of manufactured fertilizer from starfish. Report of National Fisheries Research and Development Insitute. 77: 57-74 (1989)
  2. Kishimura H, Hayashi K. Isolation and characteristics of carboxypeptidase B from the pyloric ceca of the starfish Asterias amurensis. Comp. Biochem. Physiol. B 133: 183-189 (2002)
  3. Chung K, Kim WI, Hong IK, Park KA. Ultrasonic energy effects on squalene extraction from amaranth seed. Appl. Chem. 4: 149- 152 (2000)
  4. Byon JY, Ohira T, Hirono I, Aoki T. Comparative immune responses in Japanese flounder, Paralichthys olivaceus after vaccination with viral hemorrhagic septicemia virus (VHSV) recombinant glycoprotein and DNA vaccine using a microarray analysis. Vaccine 24: 921-930 (2006)
  5. Dunsmore JD, Savini G, Robertson ID. Studies on pathogenesis, tissue infection, and congenital transmission in cows experimentally infected with Sarcocystis cruzi by various routes. Vet. Parasitol. 64: 319-327 (1996)
  6. Jin MH, Jung MH, Lim YH, Lee SH, Kang SJ, Cho WG. Promoting synthesis of collagen from Angelica dahurica root. Korean J. Pharmacogn. 35: 315-319 (2004)
  7. Maksimenko AV, Konovalova OI, Petrov AD, Arkhipova OG, Torchilin VP. Comparative study of the properties of preparations of native and modified collagenase. B. Exp. Biol. Med. 105: 342-346 (1988)
  8. Jerome SP, Gabrielle L, Raul F. Identification of collagen fibrils in scleroderman skin. J. Invest. Dermatol. 90: 48-54 (1998)
  9. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: 55-63(1983)
  10. Karesen R, Hervik A, Schlichting E. Medical logistics: Principles applied to diagnostics and therapy in women with symptoms and signs of breast cancer. Tidsskr. Norske. Laege. 123: 1687-1690 (2003)
  11. Kim JS, Kim JD, Kang MJ, Ahn HY, Kim DJ. Collagen-induced activation of MMPs (membrane-type matrix metalloproteinase and matrix metalloproteinase-2) in ovarian cancer cell lines in vitro. Korean Soc. Obstet. Gynacol. 43: 1972-1978 (2000)
  12. Kim IH, Seo JK, Kim UJ, Kim CH, Ko HJ, Kim CH, Park NK. Screening and refining of bioactive substance from Asterina pectinifera. pp. 215-216. In: Spring symposium of marine society organization. October 19, Kangnung University, Kangnung, Korea. Korea International Economic Association, Daegu, Korea (2001)
  13. Park YH, Kim MR. A study on the tyrosinase inhibitor from mushroom Phellinus ribis. Soonchunhyang J. Nat. Sci. 1: 183-188 (1995)
  14. Lee CH, Ryu TK, Chang M, Choi JW. Effect of silver, cadmium, chromium, copper, and zinc on the fertilization of the northern Pacific asteroid, Asterias amurensis. B. Environ. Contam. Tox. 73: 613-619 (2004)
  15. Benesova T, Ales H, Pilin A, Votruba J, Flieger M. A modified HPLC method for the determination of aspartic acid racemization in collagen from human dentin and its comparison with GC. J. Sep. Sci. 27: 330-334 (2004)