Characteristics and Immunomodulating Activity of Lactic Acid Bacteria for the Potential Probiotics

Probiotics로서의 젖산균주의 특성 및 면역활성

  • Seo, Jae-Hoon (Traditional Research Team, Food R&D Center, Daesang Co.) ;
  • Lee, Ho (Department of Food Science & Biotechnology, Kyonggi University)
  • 서재훈 (대상(주) 중앙연구소 식품연구실) ;
  • 이호 (경기대학교 이과대학 식품생물공학)
  • Published : 2007.12.01

Abstract

This study was designed to examine the suitable characteristics of potential probiotic bacteria. Possible probiotic bacteria, including Lactobacillus acidophilus DDS-1, Lb. acidophilus B-3208, Bifidobacterium bifidum KCTC 3357, Lb. plantarum, Leuconostoc mesenteroides ssp. mesenteroides ATCC 8293, and Lactococcus lactis ssp. lactis ATCC 7962 were selected. We then measured their acid and bile tolerances, adhesion properties in the gastrointestinal tract, antimicrobial activity against pathogenic bacteria, and immunomodulation activity. The acid tolerances of Lb. acidophilus DDS-1, Lb. acidophilus B-3208, Lb. plantarum, and Leu. mesenteroides ssp. mesenteroides ATCC 8293, in PBS (pH 2.5) for 2 hr, were high enough that 50% of the inocula survived. The bile tolerances of all bacteria, except Lc. lactis ssp. lactis ATCC 7962, were also observed at a 3% oxgall concentration in MRS broth. The results of the adhesion property assay showed that the total binding affinities of Lb. acidophilus DDS-1, Lb. acidophilus B-3208, and B. bifidum were about three times higher than those of the other bacteria. In testing their antimicrobial activities against pathogens, Lb. acidophilus B-3208, B. bifidum KCTC 3357, and Lb. plantarum inhibited the growth of pathogenic bacteria. For their immunomodulation activity, the cell wall fractions from Lb. acidophilus DDS-1 and Lb. acidophilus B-3208 showed the highest bone marrow cell proliferation activities. However, the cell wall fractions of Lb. acidophilus DDS-1 and B. bifidum, and the cytosol fraction of Lc. lactis ssp. lactis ATCC 7962 showed higher macrophage stimulation activities than those of the other bacteria. Since Lb. acidophilus DDS-1 and Lb. acidophilus B-3208 satisfy the requirements for probiotics, they can be considered suitable probiotic bacteria.

Keywords

probiotic;acid and bile tolerances;immunomodulation activity;lactic acid bacteria

References

  1. Coconnier MH, Klaenhammer TR. Kerneis S, Bernet MF, Servin AL. Protein-mediated adhesion of Lactobacillus acidophilus BG2FO4 on human enterocyte and mucus-secreting cell lines in culture. Appl. Environ. Microb. 58: 2034-2039 (1992)
  2. Matsumura A, Saito T, Arakuni M, Kitazawa H, Kawai Y, Itoh T. New binding assay and preparative trial of cell-surface lectin from Lactobacillus acidophilus group lactic acid bacteria. J. Dairy Sci. 82: 2525-2329 (1999) https://doi.org/10.3168/jds.S0022-0302(99)75505-0
  3. Zheng WH, Bastianetto S, Mennicken F, Ma W, Kar S. Amyloed $\beta$-peptide induces tau phosphorylation and loss of cholinergic neurons in rat primary septal cultures. Neuroscience 115: 201-211 https://doi.org/10.1016/S0306-4522(02)00404-9
  4. Suzuki I, Tanaka H, Kinoshita A, Oikawa S, Osawa M, Yadomae T. Effects of orally administered $\beta$-glucan on macrophage function in mice. Int. J. Immunopharmacol. 12: 675-684 (1990) https://doi.org/10.1016/0192-0561(90)90105-V
  5. Erkki S, Petaja E. Screening of commercial meat starter cultures at low pH and in the presence of bile salts for potential probiotic use. Meat Sci. 55: 297-300 (2000) https://doi.org/10.1016/S0309-1740(99)00156-4
  6. Chae OW, Shin KS, Chung HK, Choe TB. Immunostimulation effects of mice fed with cell lysate of Lactobacillus plantarum isolated from kimchi. Korean J. Biotechnol. Bioeng. 13: 424-430 (1998)
  7. Pennacchia C, Ercolini D, Blaiotta G. Pepe O, Mauriello G, Villani F. Selection of Lactobacillus strains from fermented sausages for their potential use as probiotics. Meat Sci. 67: 309-317 (2004) https://doi.org/10.1016/j.meatsci.2003.11.003
  8. Fuller R. Probiotics in man and animal. J. Appl. Bacteriol. 66:365-378 (1989) https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  9. Sanders ME. Probiotics. Food Technol.-Chicago 53: 67-77 (1999)
  10. Hong T, Matsumoto T, Kiyohara H, Yamada H. Enhanced production of hematopoetic growth factors through T cell activation in Peyer's patches by oral administration of kampo (Japanese herbal) medicine, 'Juzen-Taiho-To'. Phytomedicine 5: 353-360 (1998) https://doi.org/10.1016/S0944-7113(98)80017-2
  11. Kim JH, Shin KS, Lee H. Characterization and action mode of anti-complementary substance prepared from Lactobacillus plantarum. Korean Food Sci. Technol. 34: 290-295 (2002)
  12. Gill HS. Stimulation of immune system by lactic acid cultures. Int. Dairy J. 8: 535-544 (1998) https://doi.org/10.1016/S0958-6946(98)00074-0
  13. Shin MS, Yu KW, Shin KS, Lee H. Enhancement of immunological activity in mice with oral administration of cell wall components of Bifidobacterium bifidum. Food Sci. Biotechnol. 13: 85-89 (2004)
  14. Kim SY, Shin KS, Lee H. Immunopotentiating activities of cellular components of Lactobacillus brevis FSB-1. J. Korean Soc. Food Sci. Nutr. 33: 1552-1559 (2004) https://doi.org/10.3746/jkfn.2004.33.9.1552
  15. Lee NK, Kim TH, Choi SY, Lee SK, Park HD. Identification and probiotic properties of Lactobacillus lactis NK24 Isolation from jeotgal, a Korean fermented food. Food Sci. Biotechnol. 13: 417-420 (2004)
  16. Gill HS. Probiotics to enhance anti-infective defenses in the gastrointestinal tract. Best Prac. Res. Cl. Em. 17: 755-773 (2003) https://doi.org/10.1016/S1521-6918(03)00074-X
  17. Matsumura A, Saito T, Arakuni M, Kitazawa H, Kawai Y, Itoh T. New binding assay and preparative trial of cell-surface lectin from Lactobacillus acidophilus group lactic acid bacteria. J. Dairy Sci. 82: 2525-2329 (1999) https://doi.org/10.3168/jds.S0022-0302(99)75505-0
  18. Kenji Y, Takuya M, Hiromu T, Tomokazu N, Kyoto S, Tetsuki T, Hidehiko K. Binding specificity of Lactobacillus to glycolipids. Biochem. Biophysic. Res. Co. 228: 148-152 (1996) https://doi.org/10.1006/bbrc.1996.1630
  19. Matsumoto M, Tani H, Ono H, Ohishi H, Benno Y. Adhesive property of Bifidobacterium lactis LKM512 and predominant bacteria of intestinal microflora to human intestinal mucin. Curr. Microbiology. 44: 212-215 (2002) https://doi.org/10.1007/s00284-001-0087-4
  20. Shah NP. Probiotic bacteria: Selective enumeration and survival in dairy foods. J. Dairy Sci. 83: 894-907 (2000) https://doi.org/10.3168/jds.S0022-0302(00)74953-8
  21. Gilliland SE, Staley TE, Bush LJ. Importance of bile tolerance of Lact. acidophilus used as a dietary adjunct. J. Dairy Sci. 67: 3045-3051 (1984) https://doi.org/10.3168/jds.S0022-0302(84)81670-7
  22. Reid G, Burton J. Use of Lactobacillus to prevent infection by pathogenic bacteria. Microbes Infect. 4: 3119-324 (2002)
  23. Havenaar R, Huis in't Veld JHJ. Probiotics: A general view. Vol. 1, pp. 151-170. In: The Lactic Acid Bacteria in Health and Disease. Wood BJB (ed). Elsevier, New York, NY, USA (1992)
  24. Isolauri E, Salminen S, Ouwehand AC. Probiotics. Best Prac. Res. Cl. Em. 18: 299-313 (2004) https://doi.org/10.1016/j.bpg.2003.10.006
  25. Talwalkar A, Kailasapathy K. Comparison of selective and differential media for the accurate enumeration of strains of Lactobacillus acidophilus, Bifidobacterium ssp. and Lactobacillus casei complex from commercial yoghurts. Int. Dairy J. 14: 143-149 (2004) https://doi.org/10.1016/S0958-6946(03)00172-9
  26. Gilliland SE, Speck ML. Deconjugation of bile acids by intestinal lactobacilli. Appl. Environ. Microb. 33: 15-18 (1977)
  27. Saarela M, Lahteenmaki L, Crittenden R, Salminen S, Mattila-Sandholm T. Gut bacteria and health foods- the European perspective. Int. J. Food Microbiol. 78: 99-117 (2002) https://doi.org/10.1016/S0168-1605(02)00235-0
  28. Roy D. Media for the isolation and enumeration of bifidobacteria in dairy products. Int. J. Food Microbiol. 69: 167-182 (2001) https://doi.org/10.1016/S0168-1605(01)00496-2
  29. Saarela M, Mogensen G, Fonden R, Matto J, Mattila-Sandholm T. Probiotic bacteria: Safety, functional and technological properties. J. Biotechnol. 84: 197-215 (2000) https://doi.org/10.1016/S0168-1656(00)00375-8
  30. Kim ER, Jung HK, Juhn SL, YU JH. Factor affecting the adherence of Bifidobacteia to Caco-2 Cell. Korean J. Food Sci. Animo. Resour. 21: 133-141 (2001)
  31. Shin MS, Yu KW, Shin KS, Lee H. In vitro bone marrow cell proliferation of cell wall preparation from Bifidobacterium bifidum SL-21. Korean J. Food Sci. Technol. 36: 484-489 (2004)
  32. Shin MS, Kim HM, Kim GT, Huh CS, Bae HS, Baek YJ. Selection and characteristics of Lactobacillus acidophilus isolated from Korean feces. Korean J. Food Sci. Technol. 31: 495-501 (1999)
  33. Mukai T, Kaneko S, Matsumoto M, Ohori H. Binding of Bifidobacterium bifidum and Lactobacillus reuteri to the carbohydrate moieties of intestinal glycolipids recognized by peanut agglutinin. Int. J. Food Microbiol. 90: 357-362 (2004) https://doi.org/10.1016/S0168-1605(03)00317-9
  34. Jayaprakasha HM, Yoon YC, Paik HD. Probiotic functional dairy foods and health claims: An overview. Food Sci. Biotechnol. 13: 523-528 (2005)
  35. Kim SY, Shin KS, Lee H. Screening of lactic acid bacteria with potent adhesive property in human colon using colonic-mucin binding assay. Korean J. Food Sci. Technol. 36: 959-967 (2004)
  36. Hyronimus B, Marrec CL, Sassi AH, Deschamps A. Acid and bile tolerance of spore-forming lactic acid bacteria. Int. J. Food Microbiol. 61: 193-197 (2000) https://doi.org/10.1016/S0168-1605(00)00366-4