Protective Effects of Water Extracts Composed of Adenophora triphylla var. japonica Hara on the Acetaminophen-induced Hepatotoxicity

아세트아미노펜에 의해 유도된 간독성 모델에서 잔대를 주원료로 하는 추출물의 간 보호 효과

  • Gum, Sang-Il (College of Oriental Medicine and Research Institute of Oriental Medicine, Dongguk University) ;
  • Lee, Dong-Ung (College of Natural Science, Dongguk University) ;
  • Cho, Min-Kyung (College of Oriental Medicine and Research Institute of Oriental Medicine, Dongguk University)
  • 금상일 (동국대학교 한의과대학 약리학교실, 한의학연구소) ;
  • 이동웅 (동국대학교 자연과학대학) ;
  • 조민경 (동국대학교 한의과대학 약리학교실, 한의학연구소)
  • Published : 2007.12.01


The hepatoprotective effects of water extracts composed of Adenophora triphylla var japonica Hara (ATJH) on acetaminophen (APAP)-induced hepatotoxicity were investigated in vivo and in vivo. The effects of ATJH on liver toxicity induced by APAP were assessed by blood biochemical and histopathological analyses. APAP treatment (350 mg/kg) caused severe liver injury in mice as indicated by their significantly elevated plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. Pretreatment with ATJH for 3 or 7 days attenuated the increases in ALT and AST when challenged with APAP. The reductions in viability caused by high dose of APAP (450 mg/kg) in vivo were reversed by pretreatment with ATJH. These protective effects of ATJH against APAP-induced toxicity were consistent with the results from the histopathological examinations. We next examined the effects of ATJH on the gene expression of glutathione S-transferases (GSTs) that detoxify the metabolic intermediates of APAP in H4IIE cells. The hepatic GST protein levels [$\alpha$ class (GSTA2, GSTA3/5)] were significantly elevated in a dose-dependent manner by ATJH treatment. In summary, ATJH is effective at protecting against APAP-induced hepatotoxicity by GST induction, implying that ATJH should be considered a potential chemopreventive agent.


Adenophora triphylla var;Japonica Hara;acetaminophen;glutathione S-transferase;hepatoprotection


  1. Lee JW, Do H. Market trend of health functional food and the prospect of ginseng market. J. Ginseng Res. 29: 206-214 (2005)
  2. Kim CM, Shin MK, Lee KS, An DG. Joongyakdaesajeon (Chinese Oriental Medcine Dictionary). JuengDam. Seoul, Korea. pp. 4860-4863 (2001)
  3. Cho MK, Lee JS, Gum SI, Lee JW, Lee DW, Kim JT. A herbal mixture extract comprising adenophora tripylla and food supplement comprising the same for prevention and treatment of liver disease. Korea Patent 10-0762448 (2006)
  4. Jollow DJ, Mitchell JR, Potter WZ. Acetaminophen-induced hepatic necrosis. J. Pharmacol. Exp. Ther. 187: 195-202 (1973)
  5. Reitman S, Frankel SA, Colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transminase. Am. J. Clin. Pathol. 28: 56-63 (1957)
  6. Park EM, Ye EJ, Kim SJ, Choi HI, Bae MJ. Eliminatory effect of health drink containing Hovenia dulcis Thumb extract on ethanol- induced hangover in rats. Korean J. Food Culture 21: 71-75 (2006)
  7. Kim DH, Kim HM, Ryu JH. Pharmacological effect of Acanthopanax sessiliflorum Seeman. Oriental Med. Pharmacol. 727-732 (2005)
  8. Bray BJ, Rosengren RJ. Retinol potentiates acetaminopheninduced hepatotoxicity in the mouse: Mechanistic studies. Toxicol. Appl. Pharm. 173: 129-136 (2001)
  9. Stewart RK, Serabjit-Singh CJ, Massey TE. Gluthatione S-transferase- catalyzed conjugation of bioactivated aflatoxin B1 in rabbit lung and liver. Toxicol. Appl. Pharm. 140: 499-507 (1996)
  10. Seo KW, Kim JG, Park M, Kim TW, Kim HJ. Effects of phenethylisothiocyanate on the expression of glutathione S-transferase and hepatotoxicity induced by acetaminophen. Xenobiotica 30: 535-545 (2000)
  11. Jung YH, Ko SJ. Social-economical expenditure estimation of 5 major cause of death. J. Korean Assoc. Public Financ. 18: 23-51 (2004)
  12. Park YK, Yoo HH, Beak SH, Lee SH, Kim CM, Lee KS, Park MK, Park JH. Quality control of Adenophorae Radix Korean J. Pharmacogn. 34: 10-13 (2003)
  13. Na CS, Jeung NC, Cha GJ, Ji YS. Lower alcohol insoluble extract and polysaccharidetherein isolated from xylem of Hovenia dulcis thumb having liver protecting activity and composition containing. Korea Patent 10-2002-0025533 (2002)
  14. Park JH, Park JY, Ju YS. Inhibitory effect of licorice ethanol extracts on cytochrome P450 drug-metabolizing enzymes in human liver microsomes. Korea J. Herbol. 19: 47-54 (2004)
  15. Kim SG, Cho MK. Expression of glutathione S-transferases Ya, Yb1, Yb2, Yc1, and Yc2 and microsomal epoxide hydrolase genes by thiazole, benzothiazole, and benzothiadiazole. Biochem. Pharmacol. 52: 1831-1841 (1996)
  16. Kumar G, Banu GS, Kannan V, Pandian MR. An antihepatotoxic effect of beta-carotene on paracetamol induced hepatic damage in rats. Indian J. Exp. Biol. 43: 351-355 (2005)
  17. Thomas S. Paracetamol (acetaminophen) poisoning. J. Pharmacol. Exp. Ther. 60: 91-120 (1993)
  18. Kim DH, Kim HM, Ryu JH. Pharmacological effect of Glycyrrhiza uralensis. Oriental Med. Pharmacol. 115-121 (2005)
  19. Clapper ML. Chemopreventive activity of oltipraz. J. Parmacol. Ther. 78: 17-27 (1998)
  20. Larson AM. Acetaminophen hepatotoxicity. Clin. Liver Dis. 11: 525-548 (2007)
  21. Kim YK. Changes in the lipid composition and some enzyme activities in the rat liver as affected by diets. Korean J. Nutr. 6: 15-19 (1973)
  22. Heo SH, Kim YJ. Main contents and handbook of health functional food act. J. Food Sci. Ind. 36: 26-30 (2003)