Protective Effects of Water Extracts Composed of Adenophora triphylla var. japonica Hara on the Acetaminophen-induced Hepatotoxicity

아세트아미노펜에 의해 유도된 간독성 모델에서 잔대를 주원료로 하는 추출물의 간 보호 효과

  • Gum, Sang-Il (College of Oriental Medicine and Research Institute of Oriental Medicine, Dongguk University) ;
  • Lee, Dong-Ung (College of Natural Science, Dongguk University) ;
  • Cho, Min-Kyung (College of Oriental Medicine and Research Institute of Oriental Medicine, Dongguk University)
  • 금상일 (동국대학교 한의과대학 약리학교실, 한의학연구소) ;
  • 이동웅 (동국대학교 자연과학대학) ;
  • 조민경 (동국대학교 한의과대학 약리학교실, 한의학연구소)
  • Published : 2007.12.01

Abstract

The hepatoprotective effects of water extracts composed of Adenophora triphylla var japonica Hara (ATJH) on acetaminophen (APAP)-induced hepatotoxicity were investigated in vivo and in vivo. The effects of ATJH on liver toxicity induced by APAP were assessed by blood biochemical and histopathological analyses. APAP treatment (350 mg/kg) caused severe liver injury in mice as indicated by their significantly elevated plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. Pretreatment with ATJH for 3 or 7 days attenuated the increases in ALT and AST when challenged with APAP. The reductions in viability caused by high dose of APAP (450 mg/kg) in vivo were reversed by pretreatment with ATJH. These protective effects of ATJH against APAP-induced toxicity were consistent with the results from the histopathological examinations. We next examined the effects of ATJH on the gene expression of glutathione S-transferases (GSTs) that detoxify the metabolic intermediates of APAP in H4IIE cells. The hepatic GST protein levels [$\alpha$ class (GSTA2, GSTA3/5)] were significantly elevated in a dose-dependent manner by ATJH treatment. In summary, ATJH is effective at protecting against APAP-induced hepatotoxicity by GST induction, implying that ATJH should be considered a potential chemopreventive agent.

Keywords

Adenophora triphylla var;Japonica Hara;acetaminophen;glutathione S-transferase;hepatoprotection

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