Mixed Bacillus sp. BOD sensor

혼합 Bacillus sp. BOD 센서

  • Kang, Tae Young (Department of Chemistry, Kwangwoon University) ;
  • Park, Hyun Joo (Department of Chemistry, Kwangwoon University) ;
  • Park, Kyeong Ryang (Department of Biotechnolog, Hannam University) ;
  • Kim, Jin Doo (Department of Chemistry, Daejin University) ;
  • Cha, Geun Sig (Department of Chemistry, Kwangwoon University) ;
  • Nam, Hakhyun (Department of Chemistry, Kwangwoon University)
  • 강태영 (광운대학교 자연과학대학 화학과) ;
  • 박현주 (광운대학교 자연과학대학 화학과) ;
  • 박경량 (한남대학교 생명나노과학대학 생명공학과) ;
  • 김진두 (대진대학교 자연과학대학 화학과) ;
  • 차근식 (광운대학교 자연과학대학 화학과) ;
  • 남학현 (광운대학교 자연과학대학 화학과)
  • Received : 2006.11.14
  • Accepted : 2006.12.08
  • Published : 2007.02.28

Abstract

The BOD (biochemical oxygen demand) sensor was fabricated by covering a dissolved oxygen (DO) probe with a microbe-impregnated membrane and a dialysis membrane. Various microorganisms isolated from the soils, water and activated sludge have been evaluated for measuring biochemical oxygen demand (BOD); Bacillus species HN24 and HN93 were selected as they exhibited rapid oxygen consumption and fast recovery. Improved BOD sensor could be prepared by using mixed microbes (Bacillus subtilis, Bacillus sp. HN24 and Bacillus sp. NH93) and silicon rubber gas-permeable membrane for DO probe, and by bubbling 50% $O_2$ ($N_2$ valence) through background buffer solution. This system exhibited excellent analytical performance resulting in good linearity ($r^2=0.9986$) from 0 to 100 mg/L level of BOD.

Keywords

BOD sensor;microbial membrane;dissolved oxygen;Bacillus sp.

Acknowledgement

Supported by : 산업자원부

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