Effects of Nanocalcium Supplemented Milk on Bone Calcium Metabolism in Ovariectomized Rats

DOI QR코드

DOI QR Code

Park, H.S.;Jeon, B.J.;Ahn, J.;Kwak, H.S.

  • 투고 : 2006.10.09
  • 심사 : 2007.03.19
  • 발행 : 2007.08.01

초록

This study examined effects of calcium supplemented milk on bone loss in ovariectomized rats. Twenty four Sprague-Dawley female rats, 7 weeks-old, were divided into 4 groups, ovariectomized and fed diets containing: 1) control, no Ca supplemented milk, 2) ovx 1, Ca carbonate supplemented milk, 3) ovx 2, ionized Ca supplemented milk, and 4) ovx 3, nano Ca supplemented milk. All rats were fed 1 ml of milk containing 20 mg supplemented Ca. After 18 wk feeding, body weight gain and food efficiency ratio were significantly different between ovx 1 and ovx 3. Serum concentration of calcium and phosphorus were not different among groups. However, there was a significant difference in calcium content of dry femoral weight in ovx 3 compared with the control and ovx 2. In addition, femoral bone mineral density ($g/cm^2$) was significantly greater in ovx 3 than in other groups (p<0.05). The ovx 3 group showed the highest stiffness (N/mm), maximum energy (N) in femur and trabecular bone area (%). The present study indicated that nano Ca supplementation in milk may be an effective way to enhance bone calcium metabolism for ovariectomized rats.

키워드

Nanocalcium;Bone Ca Metabolism;Milk;Ovariectomized Rat

참고문헌

  1. Ishida, H., T. Uesugi, K. Hirai, T. Toda, H. Nukaya, K. Yokotsuka and K. Tsuji. 1998. Preventive effects of the plant isoflavones, daidzin and genistin on bone mineral density in estrogenand/ or dietary calcium-deficient rats. Bone 16:149-156.
  2. Kansal, V. K. and S. Chaudhary. 1982. Biological availability of calcium, phosphorus and magnesium from dairy products. Milchwissenschaft 37:261-263.
  3. SAS. 1985. User's Guide: Statistics, Version 5 Edition, SAS Institute Inc., Cary, NC, USA.
  4. Shen, V., R. R. Birchman, R. Xu, R. Lindsay and D. W. Dempster. 1995. Short-term changes in histomorphometric and biochemical turnover markers and bone mineral density in estrogen- and/or dietary calcium-deficient rats. Bone 16:149-156. https://doi.org/10.1016/8756-3282(95)80026-M
  5. Poneros-Schneier, A. G. and J. W. Erdman Jr. 1982. Bioavailability of calcium from sesame seeds. Almond powder, whole wheat bread, spinach and nonfat dry milk in rats. J. Food Sci. 54:150-153. https://doi.org/10.1111/j.1365-2621.1989.tb08589.x
  6. Rader, J. I., D. J. Baylink, M. R. Hughers, E. F. Safilian and M. R. Haussler. 1979. Calcium and phosphorus deficiency in rats: Effects on PTH and 1,25 dihydroxyvitamin D3. Am. J. Physiol. 236:E118-E122.
  7. Mortenson, L. and P. Charles. 1996. Bioavailability of calcium supplements and the effect of vitamin D. Am. J. Clin. Nutr. 17:1663-1670.
  8. Tamaki, H., T. Akamine, N. Goshi, H. Kurata and T. Sakou. 1998. Effects of exercise raining and etidronate treatment on bone mineral density and trabecular bone in ovariectomized rats. Bone 23:147-153. https://doi.org/10.1016/S8756-3282(98)00075-1
  9. Thomas, M. L., D. J. Simmons, L. Kidder and M. Ibarra. 1991. Calcium metabolism and bone mineralization in female rats fed marginally sufficient in calcium: Effects of increased dietary calcium intake. Bone Miner. 12:1-14. https://doi.org/10.1016/0169-6009(91)90117-I
  10. Sissons, H. A., G. J. Kelman and G. Marotti. 1984. Mechanisms of bone formation, mineralization, and enhanced resorption in calcium-deficient rats. Am. J. Physiol. 225:269-276.
  11. Wong, N. P. and D. E. Lacroix. 1980. Biological availability of calcium in dairy products. Nutr. Rep. Int. 21: 673-680.
  12. Wong, K. M., L. Singer and R. H. Ophanug. 1980. Metabolic aspects of bone resorption in calcium-deficient lactating rats. Calcif. Tissue Int. 2:213-219.
  13. Toba, Y., M. Takada, M. Tanaka and S. Aoe. 1999. Comparison of the effects of milk components and calcium source on calcium bioavailability in growing male rats. Nutr. Res. 19:449-459. https://doi.org/10.1016/S0271-5317(99)00013-5
  14. Asawa, Y., N. Amizuka, K. Hara, M. Kobayashi, M. Aita, M. Li, S. Kenmotsu, K. Oda, Y. Akiyama and H. Ozawa. 2004. Histochemical evaluation for the biological effect of menatetrenone on metaphyseal trabeculae of ovariectomized rats. Bone 35:870-880. https://doi.org/10.1016/j.bone.2004.06.007
  15. Bell, G. H., D. P. Cuthbertson and J. Orr. 1941. Strength and size of bone in relation to calcium intake. J. Physiol. 100:299-317. https://doi.org/10.1113/jphysiol.1941.sp003944
  16. Arnaud, C. D. and S. Z. Sanchez. 1990. The role of calcium in osteoporosis. Annu. Rev. Nutr. 10:397-414. https://doi.org/10.1146/annurev.nu.10.070190.002145
  17. Christiansen, C. 1992. Prevention and treatment of osteoporosis: A review of current modalities. Bone 13: S35-S39. https://doi.org/10.1016/S8756-3282(09)80008-2
  18. Hirasawa, T. N., N. Omi and I. Ezawa. 2001. Effect of 1$\alpha$- hydroxyvitamin D and egg-shell calcium on bone metabolism in ovariectomized osteoporotic model rats. J. Bone Miner. Metab. 19:84-88. https://doi.org/10.1007/s007740170045
  19. Dawson-Hughes, B. 1991. Calcium supplementation and bone loss: A review of controlled clinical trials. Am. J. Clin. Nutr. 54:274S-280S. https://doi.org/10.1093/ajcn/54.1.274S
  20. Gallagher, J. C. 1993. Pathophysiology of osteoporosis. Semin. Nephrpl. 12:109-115.
  21. Shen, V., D. W. Demster, R. Birchman, R. Xu and R. Lindsay. 1993. Loss of cancellous bone mass and connectivity in ovariectomized rats can be restored by combined treatment with PTH and estradiol. J. Clin. Invest. 91:2479-2487. https://doi.org/10.1172/JCI116483
  22. Tsuchiya, H., I. Sekiguchi and T. Kuwata. 1993. Comparison of the effects of whey mineral complexes on bone metabolism in male growing rats. J. Nutr. Sci. Vitaminol. 39:473-487. https://doi.org/10.3177/jnsv.39.473
  23. Nordin, B. E. C., A. G. Need, M. Horowiz, H. A. Morris, T. C. Durbridge and D. B. Cleghorn. 1991. Ostroporosis and calcium. Aust. NZ J. Med. 21:275-279. https://doi.org/10.1111/j.1445-5994.1991.tb00457.x
  24. Chen, K. L., M. H. Chang, S. M. Tsay, H. Y. Hurng and P. W. S. Chiou. 2006. Effects of caponization on bone characeristics and histological structure in chickens. Asian-Aust. J. Anim. Sci. 19:245-251.
  25. Li, L. L., Z. P. Hou, Y. L. Yin, Y. H. Liu, D. X. Hou, B. Zhang, G. Y. Wu, S. W. Kim, M. Z. Fan, C. B. Yang, X. F. Kong, Z. R. Tang, H. Z. Peng, D. Deng, Z. Y. Deng, M. Y. Xie, H. Xiong, P. Kang and S. X. Wang. 2007. Intramuscular administration of zinc metallothionein to preslaughter stressed pigs improves anti-oxidative status and pork quality. Asian-Aust. J. Anim. Sci. 20:761-767. https://doi.org/10.5713/ajas.2007.761

피인용 문헌

  1. 1. Physicochemical, microbial, and sensory properties of yogurt supplemented with nanopowdered chitosan during storage vol.92, pp.12, 2009, doi:10.5713/ajas.2007.1266
  2. 2. Antidiabetic activity of nanopowdered chitosan in db/db mice vol.19, pp.5, 2010, doi:10.5713/ajas.2007.1266
  3. 3. The physicochemical and sensory properties of milk supplemented with ascorbic acid-soluble nano-chitosan during storage vol.64, pp.1, 2010, doi:10.5713/ajas.2007.1266
  4. 4. Bone mineral density in postmenopausal Chinese women treated with calcium fortification in soymilk and cow's milk vol.23, pp.5, 2012, doi:10.5713/ajas.2007.1266
  5. 5. Comparison of Nanopowdered and Powdered Peanut Sprout-Added Yogurt on its Physicochemical and Sensory Properties during Storage vol.32, pp.5, 2012, doi:10.5713/ajas.2007.1266
  6. 6. Comparison of Nanopowdered and Powdered Ginseng-added Yogurt on Its Physicochemical and Sensory Properties during Storage vol.33, pp.1, 2013, doi:10.5713/ajas.2007.1266
  7. 7. Effect of Nanopowdered Peanut Sprouts on Physicochemical and Sensory Properties of Milk vol.33, pp.1, 2013, doi:10.5713/ajas.2007.1266
  8. 8. Effects of nanopowdered eggshell on postmenopausal osteoporosis: a rat study vol.23, pp.5, 2014, doi:10.5713/ajas.2007.1266
  9. 9. Influence of nanopowdered peanut sprouts in collagen-induced arthritic mice vol.23, pp.6, 2014, doi:10.5713/ajas.2007.1266
  10. 10. KARAKTERISASI FISIKOKIMIA NANOKALSIUM HASIL EKSTRAKSI TULANG IKAN NILA MENGGUNAKAN LARUTAN BASA DAN ASAM vol.25, pp.1, 2014, doi:10.5713/ajas.2007.1266
  11. 11. The Preventive Effects of Nanopowdered Peanut Sprout-added Caciocavallo Cheese on Collagen-induced Arthritic Mice vol.34, pp.1, 2014, doi:10.5713/ajas.2007.1266
  12. 12. Physicochemical and Sensory Properties of Nanopowdered Chitosan-Added Maribo Cheese during Ripening vol.34, pp.1, 2014, doi:10.5713/ajas.2007.1266
  13. 13. Absorption and Bioavailability of Nano-Size Reduced Calcium Citrate Fortified Milk Powder in Ovariectomized and Ovariectomized-Osteoporosis Rats vol.63, pp.24, 2015, doi:10.5713/ajas.2007.1266
  14. 14. Characteristics of milk tablets supplemented with nanopowdered eggshell or oyster shell vol.69, pp.3, 2016, doi:10.5713/ajas.2007.1266
  15. 15. Calcium of Spirulina platensis has Higher Bioavailability than those of Calcium Carbonate and High-calcium Milk in Sprague Dawley Rats Fed with Vitamin D-deficient Diet vol.16, pp.3, 2017, doi:10.5713/ajas.2007.1266
  16. 16. Highly bioavailable nanocalcium from oyster shell for preventing osteoporosis in rats vol.68, pp.8, 2017, doi:10.5713/ajas.2007.1266
  17. 17. Calcium Supplement Derived from Gallus gallus domesticus Promotes BMP-2/RUNX2/SMAD5 and Suppresses TRAP/RANK Expression through MAPK Signaling Activation vol.9, pp.5, 2017, doi:10.5713/ajas.2007.1266

과제정보

연구 과제 주관 기관 : NanoTechWorld