Effects of Tween 80 Pretreatment on Dry Matter Disappearance of Rice Straw and Cellulolytic Bacterial Adhesion

DOI QR코드

DOI QR Code

Lee, Chan Hee;Sung, Ha Guyn;Eslami, Moosa;Lee, Se Young;Song, Jae Y.;Lee, Sung Sill;Ha, Jong K.

  • 투고 : 2007.04.17
  • 심사 : 2007.07.08
  • 발행 : 2007.09.01

초록

An in situ experiment was conducted to find out whether Tween 80 improves rice straw digestion through increased adhesion of major fibrolytic bacteria. Rice straw was sprayed with various levels of Tween 80 non-ionic surfactant or SDS ionic surfactant 24 h before incubation in the rumen of Holstein steers. Dry matter (DM) disappearance and adhesion of F. succinogenes, R. flavefaciens and R. albus on rice straw after in situ incubation were measured by real-time PCR. Application of Tween 80 increased DM disappearance, which was more noticeable at an application level of 1% compared to lower application levels. Application of SDS resulted in an opposite response in DM disappearance with highest reduction in DM disappearance at 1% level. In a subsequent in situ experiment, higher Tween 80 was applied to rice straw in an attempt to find the optimum application level. Tween 80 at 2.5% gave better DM disappearance than 1% with a similar result at 5%. Therefore, an adhesion study was carried out using rice straw treated with 2.5% Tween 80. Our results indicated that Tween 80 reduced adhesion of all three major rumen fibrolytic bacteria to rice straw. Present data clearly show that improved DM disappearance by Tween 80 is not due to increased bacterial adhesion onto substrates.

키워드

Tween 80;SDS;Cellulolytic Bacteria;Attachment;Rice Straw

참고문헌

  1. Purdy, K. J., T. M. Embley, S. Takii and D. B. Nedwell. 1996. Rapid extraction of DNA and rRNA from sediments by a novel hydroxyapatite spin-colum method. Appl. Environ. Microbiol. 62:3905-3970
  2. SAS. 1996. SAS User's Guide (Ver. 7). SAS Inst. Inc., Cary, NC
  3. Wang, Y., T. A. McAllister, J. Baah, R. Wilde, K. A. Beauchemin, L. M. Rode, J. A. Shelford, G. M. Kamande and K. J. Cheng. 2003. Effects of Tween 80 on in vitro fermentation of silages and interactive effects of Tween 80, monensin and exogenous fibrolytic enzymes on growth performance by feedlot cattle. Asian-Aust. J. Anim. Sci. 16:968-978 https://doi.org/10.5713/ajas.2003.968
  4. Whitehouse, N. L., V. M. Olson, C. G. Schwab, W. R. Chesbro, K. D. Cunningham and T. Lykos. 1994. Improved techniques for dissociating particle-associated mixed ruminal microorganisms from ruminal digesta solids. J. Anim. Sci. 72:1335-1343 https://doi.org/10.2527/1994.7251335x
  5. Koike, S. and Y. Kobayashi. 2001. Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FEMS Microbiol Lett. 204:361-366 https://doi.org/10.1111/j.1574-6968.2001.tb10911.x
  6. Koike, S., J. Pan, Y. Kobayashi and K. Tanaka. 2003. Kinetics of in sacco fiber-attachment of representative ruminal cellulolytic bacteria monitored by competitive PCR. J. Dairy Sci. 86:1429-1435 https://doi.org/10.3168/jds.S0022-0302(03)73726-6
  7. Lee, S. S. and J. K. Ha. 2003. Influence of surfactant Tween 80 on the gas production, cellulose digestion and enzyme activities by mixed rumen microorganisms. Asian-Aust. J. Anim. Sci. 16:1151-1157 https://doi.org/10.5713/ajas.2003.1151
  8. Lee, S. S., B. H. Ahn, H. S. Kim, C. H. Kim, K. J. Cheng and J. K. Ha. 2003. Effects of non-ionic surfactants on enzyme distributions of rumen contents, anaerobic growth of rumen microbes, rumen fermentation characteristics and performance of lactating cows. Asian-Aust. J. Anim. Sci. 16:104-115 https://doi.org/10.5713/ajas.2003.104
  9. McAllister, T. A., K. Stanford, H. D. Bae, R. J. Treacher, A. N. Hristov, J. Baah, J. A. Shelford and K. J. Cheng. 2000. Effect of a surfactant and exogenous enzymes on digestibility of feed and on growth performance and carcass traits of lambs. Can. J. Anim. Sci. 80:35-44 https://doi.org/10.4141/A99-053
  10. Helle, S. S., S. J. B. Duff and D. G. Cooper. 1993. Effect of surfactants on cellulose hydrolysis. Biotechnol. Bioenginee. 42:611-617 https://doi.org/10.1002/bit.260420509
  11. Leedle, J. A. Z., K. Barsuhn and T. J. Butine. 1987. Comparison of treatments for the in vitro detachment of cellulolytic bacteria from ruminal digesta samples. Curr. Microbiol. 15:129-131 https://doi.org/10.1007/BF01577259
  12. Kamande, G. M. 1994. Manipulatin of rumen and silage fermentation. Doctoral Thesis, University of British Columbia, Vancouver, BC
  13. Kamande, G. M., J. Baah, K. J. Cheng, T. A. McAllister and J. A. Shelford. 2000. Effects of Tween 60 and Tween 80 on protease activity, thiol group reactivity, protein adsorption, and cellulose degradation by rumen microbial enzymes, J. Dairy Sci. 83:536-542 https://doi.org/10.3168/jds.S0022-0302(00)74913-7
  14. Kim, C. -H., J. N. Kim, J. K. Ha, S. G. Yun and S. S. Lee. 2004. Effects of dietary addition of surfactant Tween 80 on ruminal fermentation and nutrient digestibility of Hanwoo steers. Asian-Aust. J. Anim. Sci. 17:337-342 https://doi.org/10.5713/ajas.2004.337
  15. Kim, W., Y. Gamo, M. S. Yahaya, W. Yimiti, S. Ogawa, S. Karita and M. Goto. 2006. Effect of Tween 80 on hydrolytic activity and substrate accessibility of carbohydrolase 1 (CBH 1) from Trichoderma viride. Asian-Aust. J. Anim. Sci. 19:684-689 https://doi.org/10.5713/ajas.2006.684
  16. Baah, J., J. A. Shelford, A. N. Hristov, T. A. McAllister and K. J. Cheng. 2005. Effects of Tween80 and fibrolytic enzymes on ruminal fermentation and digestibility of feeds in holstein cows. Asian-Aust. J. Anim. Sci. 18:816-824 https://doi.org/10.5713/ajas.2005.816
  17. Dehority, B. A. and J. A. Grubb. 1980. Effect of short-term chilling of rumen contents on viable bacterial numbers. Appl. Environ. Microbiol. 39:376-381
  18. Eriksson, T., J. Börjesson and F. Tjerneld. 2002. Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose. Enzyme Microb. Technol. 31:353-364 https://doi.org/10.1016/S0141-0229(02)00134-5
  19. Goto, M., H. Bae, M. S. Yahaya, S. Karita, W. Kim, J. Baah, K. Sugawara and K. J. Cheng. 2003b. Effects of surfactant Tween 80 on enzymatic accessibility and degradation of Orchardgrass (Dactylis glomerata L.) at different growth stage. Asian-Aust. J. Anim. Sci. 16:83-87 https://doi.org/10.5713/ajas.2003.83
  20. Akin, D. E. 1980. Evaluation by electron microscopy and anaerobic culture of types of rumen bacteria associated with digestion of forage cell walls. Appl. Environ. Microbiol. 39:242-252
  21. Kim, H. S., B. S. Ahn, S. G. Chung, Y. H. Moon, J. K. Ha, I. J. Seo, B. H. Ahn and S. S. Lee. 2006. Effect of yeast culture, fungal fermentation extract and non-ionic surfactant on performance of Holstein cows during transition period. Anim. Feed Sci. Technol. 126:23-29 https://doi.org/10.1016/j.anifeedsci.2005.05.023
  22. Miron, J., D. Ben-Ghedalia and M. Morrison. 2001. Invited Review: Adhesion mechanisms of rumn cellulolytic bacteria. J. Dairy Sci. 84:1294-1309 https://doi.org/10.3168/jds.S0022-0302(01)70159-2
  23. Goto, M., H. Bae, S. S. Lee, M. S. Yahaya, S. Karita, K. Wanjae and K. J. Cheng. 2003a. Effects of surfactant Tween 80 on forage degradability and microbial growth on the in vitro rumen mixed and pure cultures. Asian-Aust. J. Anim. Sci. 16:672-676 https://doi.org/10.5713/ajas.2003.672
  24. Sung, H. G., Y. Kobayashi, J. Chang, A. Ha, I. H. Hwang and J. K. Ha. 2007. Low Ruminal pH reduces dietary fiber digestion via reduced microbial attachment. Asian-Aust. J. Anim. Sci. 20:200-207

피인용 문헌

  1. 1. Environmental Factors Affecting the Adhesion of Probiotics to Rice Fiber Fractions vol.2, pp.1-3, 2011, doi:10.5713/ajas.2007.1397

과제정보

연구 과제 주관 기관 : Ministry of Agriculture and Forestry