Effects of clove oil on haematology and blood chemistry in crusian carp (Carassius auratus)

Clove Oil의 붕어(carassius auratus) 혈액 및 혈액화학치에 대한 영향

  • Kho, Kyoung-Nam (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Jung, Tae-Sung (College of Veterinary Medicine, Gyeongsang National University) ;
  • Heo, Gang-Joon (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • 고경남 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 정태성 (경상대학교 수의과대학) ;
  • 허강준 (충북대학교 수의과대학 및 동물의학연구소)
  • Accepted : 2007.12.11
  • Published : 2007.12.30

Abstract

To determine the effects of anesthesia to clove oil on the secondary stress indices, changes in haematological and blood chemistry were monitored in healthy crucian carps (Carassius auratus). 24 fishes were divided into three groups, and blood was collected before anaesthesia (control group), immediately after anaesthesia at a concentration of 30 mg/l clove oil (anesthetized group), and 24 h after anaesthesia (recovered group). The anesthesia to clove oil significantly increased glucose in anaesthetized group, and constantly decreased lactate dehydrogenase in anaesthetized group and recovered group. However, clove oil had not effect on other biochemical indices. These results suggests that the anesthetic use of clove oil at a concentration of 30 mg/l does not cause irreversible damage in carp.

Acknowledgement

Supported by : 충북대학교

References

  1. 강정부, 권오덕, 김덕환, 김두, 나기정, 양만표, 윤화영, 이경갑, 이근우, 이정길, 이주묵, 이창우, 이채용, 정병현, 한홍율. 수의임상병리. pp.46-319, 기전연구사, 서울, 2004
  2. Abrahamsson T, Nilsson S. Effects of nerve sectioning and drugs on the catecholamine content in the spleen of the cod, Gadus morhua. Comp Biochem Physiol C 1975, 51, 223-231
  3. Adam SM. Status and use of biological indications for evaluating in effects of stress on fish. In: Adams SM (ed.). Biological Indications of Stress in Fish. pp. 1-8, American Fisheries Society, Bethesda, 1990
  4. Asztalos B, Nemcsok G, Benedeczky I, Gabriel R, Szabo A, Refaie OJ. The effects of pesticides on some biochemical parameters of carp (Cyprinus carpio L.). Arch Environ Contam Toxicol 1990, 19, 275-282 https://doi.org/10.1007/BF01056097
  5. Bonath K. Narkose der Reptilien, Amphibien und Fische. p. 359, Verlag Paul Parvey, Berlin, 1997
  6. Birnbaum MJ, Schultz J, Fain JN. Hormonestimulated glycogenolysis in isolated goldfish hepatocytes. Am J Physiol 1976, 231, 191-197
  7. Brown D, Fleming N, Balls M. Hormonal control of glucose production by Amphiuma means liver in organ culture. Gen Comp Endocrinol 1975, 27, 380-388 https://doi.org/10.1016/0016-6480(75)90205-1
  8. Brown LA. Tropical fish medicine. Anesthesia in fish. Vet Clin North Am Small Anim Pract 1988, 18, 317- 330 https://doi.org/10.1016/S0195-5616(88)50034-7
  9. Brozova V, Svobodova Z. Anaesthetics for fish. Bull Vurh Vodnany 1986, 20, 36-40
  10. Casillas E, Myers M, Ames WE. Relationship of serum chemistry values to liver and kidney histopathology in English sole (Parophrys vetulus) after acute exposure to carbon tetrachloride. Aquatic Toxicology 1983, 3, 61-78 https://doi.org/10.1016/0166-445X(83)90007-3
  11. Cho GK, Heath DD. Comparison of tricaine methanesulphonate (MS222) and clove oil anaesthesia effects on the physiology of juvenile chinook salmon Oncorhynchus tshawytscha (Walbaum). Aquae Res 2000, 31, 537-546 https://doi.org/10.1046/j.1365-2109.2000.00478.x
  12. Ferreira JT, Smit GL, Schoonbee HJ. Haematological evaluation of the anaesthetic benzocaine hydrochloride in the freshwater fish Cyprinus carpio L. J Fish Biol 1981, 18, 291-297 https://doi.org/10.1111/j.1095-8649.1981.tb03770.x
  13. Ferreira JT, Schoonbee HJ, Smit GL. The uptake of the anaesthetic benzocaine hydrochloride by the gills and the skin ofthree freshwater fish species. J Fish Biol 1984, 25, 35-41 https://doi.org/10.1111/j.1095-8649.1984.tb04848.x
  14. Fischer IU, von Unruh GE, Dengler HJ. The metabolism of eugenol in man. Xenobiotica 1990, 20, 209-222 https://doi.org/10.3109/00498259009047156
  15. Folmar LC. Effects of chemical contaminants on blood chemistry of teleost fish: A bibliography and synopsis of selected effects. Environ Toxicol Chem 1993, 12, 337-375 https://doi.org/10.1897/1552-8618(1993)12[337:EOCCOB]2.0.CO;2
  16. Gomulka P, Antychowicz J. Anaesthetics used in fish. Medycyna Wet 1999, 55, 89-93
  17. Guilderhus PA, Marking LL. Comparative efficacy of 16 anaesthetic chemicals on rainbow trout. North Am J Fish Manage 1987, 7, 288-292 https://doi.org/10.1577/1548-8659(1987)7<288:CEOACO>2.0.CO;2
  18. Iversen M, Finstad B, Mckinley RS, Eliassen RA. The efficacy of metomidate, clove oil, agui-S and benzoak as anaesthetics in Atlantic salmon stressreducing capacity. Aquaculture 2003, 221, 549-566 https://doi.org/10.1016/S0044-8486(03)00111-X
  19. Iwama GK, McGeer JC, Pawluk MP. The effects of five fish anesthetics on acid-base balance, hematocrit, blood gases, cortisol, and adrenaline in rainbow trout. Can J Zool 1989, 67, 2065-2073 https://doi.org/10.1139/z89-294
  20. Jee JH, Min EY, Park HJ, Lee OH, Keum YH, Kim SM, Woo SH, Park SI, Kang JC. Pentachlorophenol impact assessment of haematological parameters in olive flounder, Paralichthys olivaceus. J Fish Pathol 2005, 18, 81-91
  21. Jung SH, Sim DS, Park MS, Jo QT, Kim Y. Effects of formalin on haematological and blood chemistry in olive flounder, Paralichthys olivaceus (Temminck et Schlegel). Aquae Res 2003, 34, 1269-1275 https://doi.org/10.1046/j.1365-2109.2003.00936.x
  22. Kakuta I, Namba K, Uematsu K, Murachi S. Physiological response of the fish, cyprinus carpio, to formalin exposure - 1. Effects of formalin on urine flow, heart rate, respiration. Comp Biochem Physiol C 1991, 100, 405-411 https://doi.org/10.1016/0742-8413(91)90016-M
  23. Liu EH, Gibson DM. Visualization of peroxidase isozymes with eugenol, a noncarcinogenic substrate. Anal Biochem 1977, 79, 597-601 https://doi.org/10.1016/0003-2697(77)90439-0
  24. Marking LL, Meyer FP. Are better anaesthetics needed in fisheries? Fisheries 1985, 10, 2-5
  25. Maura A, Pino A, Ricci R. Negative evidence in vivo of DNA-damaging, mutagenic and chromosomal effects of eugenol. Mutat Res 1989, 227, 125-129 https://doi.org/10.1016/0165-7992(89)90008-0
  26. McDonald DG, Miligan CL. Chemical properties of the blood. In: Hoar WS, Randall DJ (eds.). Fish Physiology. pp. 55-133, Academic Press, San Diego, 1992
  27. McFarland WN. The use of anaesthetics for the handling and the transport of fishes. Calif Fish Game 1960, 46, 407-431
  28. Myszkowski L, Kaminski R, Wolnicki J. Response of juvenile tench Tinea tinea (L.) to the anaesthetic 2phenoxyethanol. J Appl Ichthyol 2003, 19, 142-145 https://doi.org/10.1046/j.1439-0426.2003.00460.x
  29. Nagababu E, Lakshmaiah N. Inhibitory effect of eugenol on non-enzymatic lipid peroxidation in rat liver mitochondria. Biochem Phannacol 1992, 43, 2393-2400 https://doi.org/10.1016/0006-2952(92)90318-D
  30. Nakano T, Tomlinson N. Catecholamine and carbohydrate concentrations in rainbow trout (Salmo gairdneri) in relation to physical disturbance. J Fish Res Bd Can 1967,24, 1701-1715 https://doi.org/10.1139/f67-140
  31. Peters G, Nubgen A, Raabe A, Mock A. Social stress induces structural and functional alterations of phagocytes in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol 1991, 1, 17-31 https://doi.org/10.1016/S1050-4648(06)80017-3
  32. Robertson L, Thomas P, Arnold CR, Trant JM. Plasma cortisol and secondary stress responses of red drum to handling, transport, rearing density, and a disease outbreak. Prog Fish Cult 1987, 49, 1-12 https://doi.org/10.1577/1548-8640(1987)49<1:PCASSR>2.0.CO;2
  33. Ross LG, Ross B. Anaesthetic and Sedative Techniques for Aquatic Animals. 2nd ed. p. 159, Blackwell Science, Oxford, 1999
  34. Scoettger RA, Julin AM. Efficacy of MS-222 as an anesthetic on four salmonids. U.S. Fish and Wildlife Service Investigation in Fish Control 13, Washington DC, 1967
  35. Soto CG, Burhanuddin S. Clove oil as a fish anaesthetic for measuring length and weight of rabbitfish (Siganus lineatus). Aquaculture 1995, 136, 149-152 https://doi.org/10.1016/0044-8486(95)01051-3
  36. Tort L, Torres P. The effects of sublethal concentrations of cadmium on haematological parameters in the dogfish, Scyliorhinus canicula. J Fish Biol 1988, 32, 277-282 https://doi.org/10.1111/j.1095-8649.1988.tb05361.x
  37. US Food and Drug Administration. Review of eugenol and related substances in flavor usage. In: Harmon JE, Gross AG (eds.). The Scientific Literature. University of Chicago Press, Chicago, 1978
  38. Velisek. J, Svobodova Z, Piackova V. Effects of clove oil anaesthesia on rainbow trout (Oncorhynchus mykiss). Acta Vet Bmo 2005, 74, 139-146 https://doi.org/10.2754/avb200574010139
  39. Velisek. J, Veliisek J, Svobodova Z, Piackova V, Groch L, Nepejchalova L. Effects of clove oil anaesthesia on common carp (Cyprinus carpio L.) Vet Med-Czech 2005, 50, 269-275
  40. Wedermeyer G. The stress of formalin treatments in rainbow trout (Salmo gairdneri) and coho salmon (Oncorhynchus kisutch). J Fish Research Board Canada 1971, 28, 1899-1904 https://doi.org/10.1139/f71-285