Upregulation of aquaporin 2 and aquaporin 4 in the water-deprived mongolian gerbil (Meriones unguiculatus) kidney

절수시 Mongolian gerbil (Meriones unguiculatus) 콩팥의 Aquaporin 2, Aquaporin 4 발현변화

  • Song, Ji-Hyun (College of Veterinary Medicine, Chungnam National University) ;
  • Kwon, Jin-Seuk (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Yong-Hwan (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Yong-Deok (Department of MedicoLife Science, Youngdong University) ;
  • Han, Ki-Hwan (School of Medicine, Ewha Womans University) ;
  • Ryu, Si-Yun (College of Veterinary Medicine, Chungnam National University) ;
  • Jung, Ju-Young (College of Veterinary Medicine, Chungnam National University)
  • 송지현 (충남대학교 수의과대학) ;
  • 권진석 (충남대학교 수의과대학) ;
  • 김용환 (충남대학교 수의과대학) ;
  • 박용덕 (영동대학교 의생명공학과) ;
  • 한기환 (이화여자대학교 의과대학) ;
  • 류시윤 (충남대학교 수의과대학) ;
  • 정주영 (충남대학교 수의과대학)
  • Accepted : 2007.11.19
  • Published : 2007.12.30


Mongolian gerbil (Meriones unguiculatus) has been as an model animal for studing the neurological disease such as stroke and epilepsy because of the congenital incompleteries in Willis circle, as well as the investigation of water metabolism because of the long time-survival in the condition of water-deprived desert condition, compared with other species animal. Aquaporin 2 (AQP2) expressed at the surface of principal cells in collecting duct results from an equilibrium between the AQP2 in intracellular vesicles and the AQP2 on the plasma membrane. Aquaporin 4 (AQP4), which is expressed in cell in a wide range of organ, is also present in the collecting duct principal cells where this is abundant in the basolateral plasma membranes and represent potential exit pathways from the cell for water entering via AQP2. In this research, we divide 3 groups of which each group include the 5 animals. In the study of 7 or 14 days water restricted condition, we investigated the AQP2 and AQP4 by using a quantitative immunohistochemistry in the kidney. The results obtained in this study were summarized as followings. AQP2 is abundant in the apical plasma membrane and apical vesicles in the collecting duct principal cell and at rare abundance in connecting tubules. In the water-deprived Mongolian gerbil kidney, expression of AQP2 was continuosly increased in the cortical collecting duct and inner medullary collecting duct. This increase was both the apical region and cytoplasm. AQP4 is mainly expressed in the inner medulla, although some expression is also noted in the more proximal segment. In the water-deprived Mongolian gerbil kidney, AQP4 was also increased in the inner medullary collecting duct. Immunoactivity was increased in entire inner medullary collecting duct and newly detected in cytoplasm of principal cell. These findings suggest that increased levels of AQP2 and AQP4 in the cortical and inner medulalry collecting duct may play a important role for maintain fluid balance in the water-deprived kidney.


Supported by : 한국학술진흥재단


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