Beagle dogs parentage testing by using 22 ISAG microsatellite markers

  • Ji, Hye-jung (Department of Veterinary Medicine, Cheju National University) ;
  • Kim, Eun-hee (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Kyoung-kap (Department of Veterinary Medicine, Cheju National University) ;
  • Kang, Tae-young (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Joo-myoung (Department of Veterinary Medicine, Cheju National University) ;
  • Shin, Hyoung-doo (Department of Genetic Epidmiology, SNP Genetics) ;
  • Kim, Lyoung-hyo (Department of Genetic Epidmiology, SNP Genetics) ;
  • Yun, Young-min (Department of Veterinary Medicine, Cheju National University)
  • Accepted : 2007.11.26
  • Published : 2007.12.30

Abstract

The objective of the study was to establish routine parentage testing system in Beagle dogs using 22 ISAG (International Society for Animal Genetics) canine microsatellite markers (2005). Blood collections were obtained from a mother dog, 4 candidate father dogs and 3 offspring (n = 8). Genomic DNA samples were extracted from 8 Beagle dogs blood for PCR analysis. PCR products for the allele were analyzed by ABI 3130 DNA Sequencer and GeneScan (Ver 3.0) analysis and Genotyper (Ver. 2.1) software. The genetic relationship of mother and 3 offspring as well as one father dog among 4 candidate father dogs was confirmed by microsatellite allele analysis. The results of locus for amelogenin, which was designed for sexing, were matching with real gender among 8 Beagle dogs (female; 217/217 homozygosity, male; 179/217 heterozygosity). Twenty two ISAG microsatellite markers are useful the parentage test of Beagle dogs. In addition, amelogenin is an applicable marker to detecting real sex in dogs.

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