Identification of Single Nucleotide Polymorphism of H-FABP Gene and Its Association with Fatness Traits in Chickens

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Wang, Yan;Shu, Dingming;Li, Liang;Qu, Hao;Yang, Chunfen;Zhu, Qing

  • 투고 : 2007.04.17
  • 심사 : 2007.07.22
  • 발행 : 2007.12.01

초록

Heart fatty acid-binding protein gene (H-FABP) is an important candidate gene for meat quality. One of the objectives of this study was to screen single nucleotide polymorphisms (SNP) of chicken H-FABP gene among 252 individuals that included 4 Chinese domestic chicken breeds (Fengkai Xinghua (T04), Huiyang Huxu (H), Qingyuan Ma (Q), Guangxi Xiayan (S1)), 2 breeds developed by the Institute of Animal Science, Guangdong Academy of Agricultural Sciences (Lingnan Huang (DC), dwarf chicken (E4)) and one introduced broiler (Abor Acre (AA)). Another objective of this study was to analyze the associations between polymorphisms of the H-FABP gene and fat deposition traits in chickens. PCR-SSCP was used to analyze SNPs in H-FABP and 4 SNPs (T260C, G675A, C783T and G2778A) were detected. Associations between polymorphic loci and intramuscular fat (IMF), abdominal fat weight (AFW) and abdominal fat percentage (AFP) were analyzed by ANCOVA method. The results showed that the T260C genotypes were significantly associated with IMF (p = 0.0233) and AFP (p = 0.0001); the G675A genotypes were significantly associated with AFW, AFP (p<0.01) and IMF (p<0.05); at the C783T locus, AFW and AFP differed highly between genotypes. However, the G2778A loci did not show any significant effect on fat deposition traits in this study. In addition, we found that there were some differences between AFP and definite haplotypes through a nonparametric statistical method, so the haplotypes based on the SNPs except G2778A loci were also significantly associated with IMF, AFW (g) (p<0.05) and AFP (%) (p<0.001). Significantly and suggestively dominant effects of H4H4 haplotype were observed for IMF and the H2H3 was dominant for AFW (g) and AFP (%). The results also revealed that H5H7 haplotype had a negative effect on IMF, while the H5H6 had a positive effect on AFW (g) and AFP (%).

키워드

Chicken;H-FABP Gene;Single Nucleotide Polymorphism (SNP);Fat Deposition Traits;PCR-SSCP

참고문헌

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피인용 문헌

  1. 1. Relationships Between Single Nucleotide Polymorphisms of the H-FABP Gene and Slaughter and Meat Quality Traits in Chicken vol.47, pp.7-8, 2009, doi:10.5713/ajas.2007.1812
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  3. 3. Correlation between Heart-type Fatty Acid-binding Protein Gene Polymorphism and mRNA Expression with Intramuscular Fat in Baicheng-oil Chicken vol.28, pp.10, 2015, doi:10.5713/ajas.2007.1812
  4. 4. Polymorphisms in the Perilipin Gene May Affect Carcass Traits of Chinese Meat-type Chickens vol.28, pp.6, 2015, doi:10.5713/ajas.2007.1812
  5. 5. Association of H-FABP gene polymorphisms with intramuscular fat content in Three-yellow chickens and Hetian-black chickens vol.7, pp.1, 2016, doi:10.5713/ajas.2007.1812
  6. 6. Correlation of the A-FABP Gene Polymorphism and mRNA Expression with Intramuscular Fat Content in Three-Yellow Chicken and Hetian-Black Chicken vol.28, pp.1, 2017, doi:10.5713/ajas.2007.1812

과제정보

연구 과제 주관 기관 : Natural Science Research Fund of Guangdong Province