The Yield and Composition of Milk from Transgenic Rabbits

  • Chrenek, P. (Slovak Agricultural Research Centre) ;
  • Chrastinova, L. (Slovak Agricultural Research Centre) ;
  • Kirchnerova, K. (Slovak Agricultural Research Centre) ;
  • Makarevich, A.V. (Slovak Agricultural Research Centre) ;
  • Foltys, V. (Slovak Agricultural Research Centre)
  • Received : 2006.03.23
  • Accepted : 2006.06.20
  • Published : 2007.04.01


Basic objective of this research was to compare the milk yield and composition of New Zealand White transgenic rabbit females expressing recombinant human factor VIII (hFVIII) in mammary gland during lactation with that of non-transgenic rabbit females of the same age during 30 days of lactation. Transgenic founders were generated by the microinjection of foreign DNA (mWAP-hFVIII gene construct) into the egg. F1, F2 and F3 generations of transgenic rabbits were obtained after mating of transgenic founder rabbits with non-transgenic rabbits. The amount of milk rejected was measured by weight-suckle-weight method at $10^{th}$, $20^{th}$and $30^{th}$ day of lactation. Quality of milk (content of fat, protein, lactose, dry ash, and some minerals) from transgenic and non-transgenic rabbit was also determined. Comparison of milk yield, determined by weight-suckle-weight method, showed significantly higher (p<0.05) milk production at day 20 of first lactation in non-transgenic females, but on the same day of second lactation higher milk yield was measured in transgenic ones. Significantly higher (p<0.05) content of milk fat and protein was determined in transgenic milk whilst higher content of lactose was found in non-transgenic milk. The content of minerals (calcium, phosphorus, magnesium and sodium) did not differ in both experimental and control groups. Our results showed that milk yield and composition of transgenic rabbit females (mammary specific transgenic over-expression of hFVIII) over several generations is only slightly and transiently different from milk yield of non-transgenic females, which had no significant consequence on the litter size and viability.


Rabbit;Transgenic;Milk Yield;mWAP-hFVIII Gene


  1. Barash, I., A. Faerman, M. Richenstein, R. Kairi, G. M. Damary, M. Shani and M. J. Bissell. 1999. In vivo and in vitro expressin of human serum albumin genomic sequences in mammary epithelial cells with $\beta$-lactoglobuline and whey acid protein promoters. Molec. Reprod. Devel. 52:241-252.<241::AID-MRD1>3.0.CO;2-X
  2. Chrenek, P., D. Vasicek, A.V. Makarevich, R. Jurcik, K. Suvegova, M. Bauer, V. Parkanyi, J. Rafay, A. Batorova and R. K. Paleyanda. 2005a. Increased transgene integration efficiency upon microinjection of DNA into both pronuclei of rabbit embryos. Transgenic Res. 14:417-428.
  3. Chrastinova, L., A. Sommer, J. Rafay and M. Svetlanska. 1997 Avotan exploitation in rabbit nutrition. II. Nutrient digestibility and lactation performance of does rabbit. J. Farm Anim. Sci. 30:80-86.
  4. Dragin, S., J. Pivko, P. Massanyi, N. Lukac, A. Makarevich and P. Chrenek. 2006. Ultrastructural morphometry of mammary gland in transgenic and non-transgenic rabbits. Anat. Histol. Embryol., 35:in press
  5. Jenness, J. P. 1982. Inter-species comparison of milk proteins. Dev. Diary Chem. 1:87-114.
  6. Schranner, S. 1993. Untersuchungen zum maschinellen Milchentzug beim Kaninchen als Grandlage zur Bestimmung von Laktationsleistungen und Milchinhaltsstoffen. Inaugural- Dissertation, Ludwig-Maximilians-Universitat Munchen, Germany, pp 142.
  7. Van Cott, E. K., H. Lubon, F. C. Gwazdauskas, J. J. Knight, W. N. Drohan and W. H. Velander. 2001. Recombinant human protein C expression in milk of transgenic pigs and effect on endogenous milk immunoglobulin and transferin levels. Transgenic Res. 10:43-51.
  8. Yalcin, S., E. E. Onbasilar and I. Onbasilar. 2006. Effect of sex on carcass and meat characteristic of new Zealand white rabbits aged 11 weeks. Asian-Aust. J. Anim. Sci. 19(8):1212-1216.
  9. Garber, K. 2000. RhFVIII deficit questioned. Nat. Biotechnol. 18:133-134.
  10. Lukefahr, S., W. D. Hohenboken, P. R. Cheeke and N. M. Patton. 1983. Doe reproduction and preweaning litter performance of straightbred and crossbred rabbits. J. Anim. Sci. 57:1090-1096.
  11. Drummond, H., E. Vazquez, S. Sanchez-Colon, M. Martinez- Gomez and R. Hudson. 2000. Competition for milk in the domestic rabbit: Survivors benefit from littermate deaths. Ethol. 106:511-526.
  12. Sanchez, W., P. R. Cheeke and N. M. Patton. 1985. Effect of dietary crude protein level on the reproductive performance and growth of New Zealand White rabbits. J. Anim. Sci. 60:1029-1039.
  13. Monaco, M. H., D. E. Gronlund, G .T. Bleck, W. L. Hurley, M. B. Wheeler and S. M. Donovan.2005. Mammary specific transgenic over-expression of insulin-like growth factor-I (IGF-I) increase pig milk IGF-I and IGF binding proteins, with no effect on milk composition or yield. Transgenic Res. 14:761-773.
  14. Chrenek, P., V. Kaufmann, A.V. Makarevich, M. Bauer, L. Ryban, P. Uhrin, R.K. Paleyanda and B. R. Binder. 2005b. Production of recombinant human clotting Factor VIII in mammary gland of two transgenic rabbit lines. 49th GTH meeting in Mannheim, February, poster p. 118.
  15. Fraga, M. J., M. Lorente, R. M. Carabano and J. C. De Blas. 1989. Effect of diet and of remaining interval on milk production and milk composition of the doe rabbit. Anim. Prod. 48:459-465.
  16. Partridge, G. G. 1986. Meeting the protein and energy requirements of the commercial rabbit for growth and reproduction. Proc. 4th World congress of Animal Feeding. Madrid, Spain, pp. 271-277.
  17. Dragin, S., A. Bozic and P. Chrenek. 2004. Effect of transgenesis on F2 and F3 rabbit offspring generations. 5th. scientific conference of PhD. students, University of Constantine Philosophie, Nitra, Slovakia, pp. 28-32.
  18. Lukefahr, S., W. D. Hohenboken, P. R. Cheeke, N. M. Patton. 1983. Characterization of straightbred and crossbred rabbits for milk production and associative traits. J. Anim. Sci. 57:1100-1107.
  19. Wilde, C. J., A. J. Clark, A. M. Kerr, C. M. Knight, M. McClenaghan and J. P. Simons. 1992. Mammary development and milk secretion in transgenic mice expressing the sheep beta-lactoglobulin gene. Biochem. J. 284:717-720.
  20. Bautista, A., M. Mendoza-Degante, G. Coureaud, M. Martinez- Gomez and R. Hudson. 2005. Scramble competition in newborn domestic rabbts for an unusually restricted milk supply. Anim. Behaviour 70:1011-1021.
  21. Palmer, C. A., H. Lubon and L. McManaman. 2003. Transgenic mice expressing recombinant human protein C exhibit defects in lactation and impaired mammary gland development. Transgenic Res. 12:283-292.
  22. Davies, M. 1983. The composition of milk. Biochem. Lactation, T.B.Mepham. Elsevier, pp. 71-117.
  23. Taranto, S., C. Di Meo, G. Stanco, G. Piccolo, M. P. Gazaneo and A. Nizza. 2003. Influence of age at weaning on caecal content characteristics and post-weaning performance and health of rabbits. Asian-Aust. J. Anim. Sci. 16(10):1540-1544
  24. Salvo-Garrido, H., S. Travella, L. J. Bilham, W. A. Harwood and J. W. Snape. 2004. The distribution of transgene insertion sites in barley determined by physical and genetic mapping. Genet, 167:1371-1379.

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