Retroviral Gene Expression in Spermatogonial Stem Cells during Long-term Culture

  • Jeong, Dong Kee (Department of Animal Science and Biotechnology, College of Applied Life Sciences Cheju National University) ;
  • Griswold, Michael D. (School of Molecular Biosciences, Center for Reproductive Biology, Washington State University)
  • Received : 2006.11.27
  • Accepted : 2007.05.20
  • Published : 2007.07.01


The spermatogonial stem cell (SSCs) is unique in that it is the only cell in the adult male that can contribute genes to a subsequent generation. Permanent modification of the germ cell line may be realized if stem cells could be cultured, transfected with unique genes, and then transplanted into recipient testes. We developed a culture system that supported long-term viability of SSCs. We used a retrovirus vector (pMSCV including ${\beta}$-galactosidase) to stably transfect spermatogonia following long-term culture using the system developed. Expression of the reporter gene ${\beta}$-galactosidase controlled by the retroviral vector was stable in long-term cultured SSCs. We confirmed the retroviral-mediated ${\beta}$-galactsidase gene could be expressed in germ cells in recipient mice following SSCs transplantation.


Retrovirus;Gene Transfer;Electroporation;Stem Cell Medium


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