Antimicrobial susceptibility and genetic characteristics of Streptococcus uberis isolated from bovine mastitis milk

젖소 유방염 유즙에서 분리한 Streptococcus uberis의 항생제 감수성 및 유전학적 특성

  • Lee, Gil (Department of Dairy Science, Konkuk University) ;
  • Kang, Hyun-Mi (National Veterinary Research and Quarantine Service) ;
  • Chung, Chung-il (Department of Dairy Science, Konkuk University) ;
  • Moon, Jin-San (National Veterinary Research and Quarantine Service)
  • 이길 (건국대학교 낙농학과) ;
  • 강현미 (국립수의과학검역원) ;
  • 정충일 (건국대학교 낙농학과) ;
  • 문진산 (국립수의과학검역원)
  • Accepted : 2007.01.16
  • Published : 2007.03.30


Streptococcus spp. comprising Streptococcus (S.) uberis S. dysgalactiae strains is major causeof bovine mastitis from particularly well-managed or low somatic cell count herds that have successfullycontrolled contagious pathogens. In this study, antimicrobial susceptibility and genetic characteristics of S.uberis isolated from clinical or subclinical mastitis milk at 2003 were investigated. Eighty seven isolatesof Streptococus spp. were identified by the conventional biochemical methods. The antimicrobialsusceptibility by disk diffusion method was determined for 46 S. uberis, 11 S. bovis, 10 S. oralis, 6 S.uberis and 14 other Streptococcus spp.. Overall, the tested strains were susceptible to tetracycline (11.5%),amikacin (14.9%), streptomycin (16.1%), neomycin (26.4%), kanamycin (35.6%), gentamicin (65.2%),oxacillin (70.1%), ampicillin (75.9%), chloramphenicol (78.2%), and cephalothin (97.7%). Additionally, S.uberis strains were susceptible to pencillin G (97.8%), but resistant to erythromycin (76.0%) by minimalinhibitory concentration test. The multiple-drug resistance rate of isolated bacteria to 4 more thanamplification fingerprinting patterns amplifed with primer 8.6d showed that 3 to 8 number of distinguishableDNA fragments ranged from 180 bp to 1,20 bp. Thirty seven isolates of S. uberis strains were subtypedinto 8 distinct patterns. Each subtype revealed a typical pattern of antimicrobial susceptibilities. Thesefindings demonstrate that S. uberis isolates were mastitis pathogens of diverse serotypes, and oftenencountered the diverse resistant patterns.


  1. 강희장, 김익천, 김진희, 손원근, 이두식. 젖소의 원인균 분리 및 약제 감수성 검사. 대한수의학회지 2001, 41, 511-521
  2. 김 두. 유우의 임상형 유방염 원인균과 항생제 감수성의 변화 양상. 대한수의학회지 1988, 28, 397-404
  3. 김충희, 김곤섭, 허정호, 점명호, 김국헌, 조명희, 이국천, 류재두, 하대식, 김종수. 경남 남부지방에서 임상형 유방염의 원인균 분리 및 약제 감수성 시험. 한국임상수의학회지 2003, 20, 177-184
  4. 손봉환. 유방염 감염 조사 및 예방대책에 관한 연구. (최근 3년간('91-'93) 유방염 발생 실태에 관한 최종 결과 보고서), 한국가축위생학회, 서울, 1994
  5. 손봉환, 김효민, 한웅주, 정흥환, 김수장. 경기도지역의 유우 유방염에 관한 조사. 4. 유우 유방염에서 분리한 Staphylococcus와 Streptococcus의 항균제에 대한 감수성시험. 대한수의학회지 1975, 15, 101-108
  6. 송영민, 김기일, 김창수, 최석호, 윤영호. 환경형 유방염 원인균 Streptococcus uberis의 Genomic DNA Typing과 항생제 감수성. 한국동물자원과학회지 2000, 42, 93-100
  7. 이정치, 이채용, 김상기, 이정길, 서국현. Holstein 유우의 유즙에서 분리한 유방염 원인균의 항균제 감수성. 한국임상수의학회지 2003, 20, 166-171
  8. 정창국, 한홍율, 정길택. 우리나라 젖소 유방염 원인균 역학조사 및 치료에 관한 연구. 대한수의학회지 1970, 10, 39-45
  9. 한홍율, 박선일, 유종현. 항생제 내성에 관한 수의학적 고찰. 대한수의사회지 1998, 34, 483-489
  10. Anon. Veterinary Investigation Surveillance Report, Veterinary Laboratories Agency. London, 2001
  11. Bradley AJ. Bovine mastitis: an evolving disease. Vet J 2002, 164, 116-128
  12. Bramley AJ, Dodd FH. Reviews of the progress of dairy science: mastitis control-progress and prospects. J Dairy Res 1984, 51, 481-512
  13. Clinical and Laboratory Standards Institute. Performance Standards for antimicrobial disk susceptibility tests for bacteria isolated from animals. 2nd, NCCLS document M32-A2, Wayne, Pennsylvania, 2002
  14. Dodd FH, Westgarth DR, Griffin TK. Strategy of mastitis control. J Am Vet Med Assoc 1977, 170, 1124-1128
  15. Gurin-Fauble V, Tardy F, Bouveron C, Carret G. Antimicrobial susceptibility of Streptococcus species isolated from clinical mastitis in dairy cows. Int J Antimicrob Agents 2002, 19, 219-226
  16. Hogan JS, Smith KL, Hoblet KH, Schoenberger PS, Todhunter DA, Hueston WD, Pritchard DE, Bowman GL, Heider LE, Brockett BL, Conrad HR. Field survey of clinical mastitis in low somatic cell count herds. J Dairy Sci 1989, 72, 1547-1556
  17. Hookey JV, Richardson JF, Cookson BD. Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene. J Clin Microbiol 1998, 36, 1083-1089
  18. Leigh JA. Streptococcus uberis: a permanent barrier to the control of bovine mastitis. Vet J 1999, 157, 225-238
  19. Jayarao BM, Bassam BJ, Caetano-Anolles G, Gresshoff PM, Oliver SP. Subtyping of Streptococcus uberis by DNA amplification fingerprinting. J Clin Microbiol 1992, 30, 1347-1350
  20. King JS. Streptococcus uberis: a review of its role as a causative organism of bovine mastitis. I. Characteristics of the organism. Br Vet J 1981, 137, 36-52
  21. Mackey ET, Hinton M. The survival of streptococci and enterococci in animal feed and on straw with particular reference to Streptococcus uberis. J Appl Bacteriol 1990, 68, 345-348
  22. Oliver SP. Frequency of isolation of environmental mastitis-causing pathogens and incidence of new intramammary infection during the nonlactating period. Am J Vet Res 1988, 49, 1789-1793
  23. Pankey JW, Pankey PB, Barker RM, Williamson JH, Woolford MW. The prevalence of mastitis in primiparous heifers in eleven Waikato dairy herds. N Z Vet J 1996, 44. 41-44
  24. Philpot WN. Economics of mastitis control. Vet Clin North Am large Anim Pract 1984, 6, 233-245
  25. Phuektes P, Mansell PD, Dyson RS, Hooper ND, Dick JS, Browning GF. Molecular epidemiology of Streptococcus uberis isolates from dairy cows with mastitis. J Cli Microbiol 2001, 39, 1460-1466
  26. Rossitto PV, Ruiz L, Kikuchi Y, Glenn K, Luiz K, Watts JL, Cullor JS. Antibiotic susceptibility patterns for environmental streptococci isolated from bovine mastitis in central California dairies. J Dairy Sci 2002, 85, 132-138
  27. Salmon SA, Watts JL, Aarestrup FM, Pankey JW, Yancey RJ Jr. Minimum inhibitory concentrations for selected antimicrobial agents against organisms isolated from the mammary glands of dairy heifers in New Zealand and Denmark. J Dairy Sci 1998, 81, 570-578
  28. Smith KL, Hogan JS. Environmental mastitis. Vet Clin North Am Food Anim Pract 1993, 9, 489-498
  29. Watts JL. Etiological agents of bovine mastitis. Vet Microbiol 1988. 16, 41-66
  30. Williamson JH, Woolford MW, Day AM. The prophylactic effect of a dry-cow antibiotic against Streptococcus uberis. N Z Vet J 1995, 43, 228-234
  31. Zadoks RN. Tikofsky LL, Boor KJ. Ribotyping of Streptococcus uberis from a dairy's environment, bovine feces and milk. Vet Microbiol 2005, 109, 257-265