Levels of sulfonamides for animals in food

식품 중 설폰아마이드계 동물용의약품의 잔류실태

  • Jeong, Jiyoon (Residue & Chemicals Team, Korea Food & Drug Administration) ;
  • Hong, Mooki (Residue & Chemicals Team, Korea Food & Drug Administration) ;
  • Choi, Dongmi (New Hazard Chemicals Team, Korea Food & Drug Administration)
  • 정지윤 (식품의약품안전청, 잔류화학물질팀) ;
  • 홍무기 (식품의약품안전청, 잔류화학물질팀) ;
  • 최동미 (식품의약품안전청, 신종유해물질팀)
  • Received : 2006.03.03
  • Accepted : 2007.02.12
  • Published : 2007.02.28

Abstract

To determine levels of 11 sulfonamides for animals in food, simultaneously, a selective method of high performance liquid chromatography with UV detector has been applied. The targets were sulfachlorpyridazine (SCP), sulfadiazine (SDZ), sulfadimethoxine (SDM), sulfisoxazole (SSX), sulfamerazine (SMZ), sulfamethazine (SMT), sulfamethoxazole (SMX), sulfamethoxypyridazine (SMP), sulfamonomethoxine (SMM), sulfaquinoxaline (SQX) and sulfathiazole (STZ). Food samples were beef, pork, chicken, milk and whole egg that were collected at the main 6 cities in Korea as Seoul, Busan, Daejon, Incheon, Mokpo and Gangneung. After homogenizing food samples with sodium phosphate solution and acetonitrile, it was extracted with n-hexane. The mobile phase gradient was a mixture of 5 mM potassium phosphate (pH 3.25) and methanol with a gradient ratio from 100:0 to 30:70. The UV wavelength was 270 nm. The overall recoveries were ranged from 75% to 95% and the limit of detection was minimum 0.004 mg/kg for SMT, and 0.007 mg/kg for STZ at signal/noise > 3, respectively. As results, sulfonamide drugs were not detected in most of the selected food samples, however, sulfamonomethoxine was detected in meat. The determined level of sulfamonomethoxine were 0.03 and 0.06 mg/kg for beef that were below the MRLs.

Keywords

sulfonamides;veterinary medicine;food;HPLC

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