Association between PCR-RFLP Polymorphisms of Five Gene Loci and Milk Traits in Chinese Holstein

DOI QR코드

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Zhang, R.F.;Chen, H.;Lei, C.Z.;Fang, X.T.;Zhang, Y.D.;Hu, S.R.;Su, L.H.

  • 투고 : 2006.03.21
  • 심사 : 2006.08.22
  • 발행 : 2007.02.01

초록

The objective of this study was to assess the association of polymorphisms in ${\kappa}$-cn, ${\beta}$-lg, ${\beta}$-lg 5′ flanking region, CSN1S2, and IGFBP-3 genes with milk production traits and mastitis-related traits in Chinese Holstein. Traits analyzed were 305 day standard milk yield, protein percentage, fat percentage, the ratio of fat percentage and protein percentage, pre-somatic cell count, somatic cell count, and somatic cell score, respectively. CSN1S2 locus was uninformative because only one genotype BB was found in Chinese Holstein. Allele frequencies of A and B in IGFBP-3 gene were 0.5738 and 0.4262 in Chinese Holstein population, which was different from reported Qinchuan cattle population. The genotypes of animals at IGFBP-3 locus significantly affected 305 day standard milk yield, protein percentage, and somatic cell score. The ${\beta}$-lg genotypes had a significant effect on protein percentage and the ratio of fat percentage and protein percentage. Polymorphism in ${\beta}$-lg 5′ flanking region was associated with 305 day standard milk yield, protein percentage, fat percentage, pre-somatic cell count, and somatic cell count. No significant associations of the polymorphism in ${\kappa}$-cn gene were observed for any trait.

키워드

Chinese Holstein;${\beta}$-lg;${\kappa}$-cn;IGFBP-3;Milk Traits

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