- Volume 20 Issue 7
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Interaction of Bovine Growth Hormone with Buffalo Adipose Tissue and Identification of Signaling Molecules in Its Action
- Sodhi, R. (Animal Biochemistry Division, National Dairy Research Institute) ;
- Rajput, Y.S. (Dairy Chemistry Division, National Dairy Research Institute)
- Received : 2006.04.18
- Accepted : 2006.09.12
- Published : 2007.07.01
Results on localization of growth hormone receptor (GHR), interaction of growth hormone (GH) with receptor in buffalo adipose tissue and identification of activated signaling molecules in the action of GH are presented. Bovine GH (bGH) was labeled with fluorescein or biotin. Fluorescein-labelled bGH was used for localization of GHRs in buffalo adipocytes. The receptors were present on the cell surface. The affinity of binding of GH to its receptor was determined by designing an experiment in which buffalo adipose tissue explants, biotinylated GH and streptavidin-peroxidase conjugate were employed. The affinity constant was calculated to be
Growth Hormone;Growth Hormone Receptor;Signalling Molecules;Buffalo;Jak2
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