Influence of Dietary Salinomycin on Feeding-induced Variations of Glucose Kinetics and Blood Volatile Fatty Acids and Insulin Concentrations in Sheep Fed a High-roughage Diet

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Fujita, Tadahisa;Itoh, Takahiro;Majima, Hiroya;Sano, Hiroaki

  • 투고 : 2006.04.17
  • 심사 : 2006.07.03
  • 발행 : 2007.03.01

초록

This study was conducted to determine effects of salinomycin (SL) on feeding-induced changes in glucose kinetics and blood VFA concentrations in sheep fed a high-roughage diet. Four sheep were fed the diet with or without 20 mg/kg diet of SL once daily for 21 d. Glucose entry and utilization rates were determined during the prefeeding and 3 h postfeeding periods, using a [$^{13}C_6$]glucose dilution method and non-steady state equations. Ruminal characteristics and concentrations of blood VFA, plasma glucose and insulin were also measured during the same periods. A feeding-induced increase in ruminal total VFA concentration tended to be inhibited (p<0.10) with SL, although ruminal pH was unaffected (p>0.10) with SL or by feeding. Salinomycin decreased (p<0.05) acetate proportion and increased (p<0.05) propionate proportion in the rumen, but did not modify these changes in response to feeding (p>0.10). A feeding-induced increase in blood acetate concentration was attenuated (p<0.05) with SL. Salinomycin tended to increase (p<0.10) blood propionate concentration without modifying its response patterns to feeding (p>0.10). Plasma concentrations of glucose or insulin were unaffected (p>0.10) with SL. Salinomycin tended to enhance (p<0.10) glucose entry and utilization rates. Feeding also enhanced (p<0.01) both rates, whereas their interactive effect was not detected (p>0.10). We conclude that SL possibly enhances whole body glucose entry and utilization with an increase in blood propionate concentration in sheep given a high-roughage diet, although SL does not appear to affect their responses to feeding.

키워드

Feeding;Glucose Metabolism;Insulin;Salinomycin;Sheep;Volatile Fatty Acid

참고문헌

  1. Brockman, R. P. 1990. Effect of insulin on the utilization of propionate in gluconeogenesis in sheep. Br. J. Nutr. 64:95-101. https://doi.org/10.1079/BJN19900012
  2. Cowan, J. S. and G. Hetenyi Jr. 1971. Glucoregulatory responses in normal and diabetic dogs recorded by a new tracer method. Metabolism 20:360-372. https://doi.org/10.1016/0026-0495(71)90098-9
  3. National Research Council. 1985. Nutrient Requirements of Sheep, sixth rev. Ed. National Academy Press, Washington, DC.
  4. Pethick, D. W., D. B. Lindsay, P. J. Barker and A. J. Northrop. 1981. Acetate supply and utilization by the tissues of sheep in vivo. Br. J. Nutr. 46:97-110. https://doi.org/10.1079/BJN19810013
  5. Prange, R. W., C. L. Davis and J. H. Clark. 1978. Propionate production in the rumen of Holstein steers fed either a control or monensin supplemented diet. J. Anim. Sci. 46:1120-1124. https://doi.org/10.2527/jas1978.4641120x
  6. Reffett-Stabel, J., J. W. Spears, R. W. Harvey and D. M. Lucas. 1989. Salinomycin and lasalocid effects on growth rate, mineral metabolism and ruminal fermentation in steers. J. Anim. Sci. 67:2735-2742. https://doi.org/10.2527/jas1989.67102735x
  7. Sano, H., A. Takebayashi, Y. Kodama, K. Nakamura, H. Ito, Y. Arino, T. Fujita, H. Takahashi and K. Ambo. 1999. Effects of feed restriction and cold exposure on glucose metabolism in response to feeding and insulin in sheep. J. Anim. Sci. 77:2564-2573. https://doi.org/10.2527/1999.7792564x
  8. SAS Insutitute Inc. 1996. $SAS/STAT^{\circledR}$ Software: Changes and Enhancements through Release 6.11. SAS Institute Inc., Cary, North Carolina.
  9. Singh, G. P. and D. De. 2005. Effect of different levels of monensin supplemented with cold process urea molasses mineral block on in vitro rumen fermentation at different adaptation with monensin. Asian-Aust. J. Anim. Sci. 18:320-325. https://doi.org/10.5713/ajas.2005.320
  10. Tserng, K.-Y. and S. C. Kalhan. 1983. Estimation of glucose carbon recycling and glucose turnover with [$U-^{13}C$]glucose. Am. J. Physiol. 245:E476-E482.
  11. Fujita, T., T. Itoh, H. Majima, H. Sano, A. Shiga and K. Ambo. 2000. Effects of type of diet and salinomycin supplementation on insulin secretory response and action in sheep. Anim. Sci. J. 71:42-49.
  12. Armentano, L. E. and J. W. Young. 1983. Production and metabolism of volatile fatty acids, glucose and $CO_2$ in steers and the effects of monensin on volatile fatty acid kinetics. J. Nutr. 113:1265-1277. https://doi.org/10.1093/jn/113.6.1265
  13. Terashima, Y., T. Kuroyanagi, Y. Miyakoshi, Y. Fukuda and Y. Kondo. 1990. The effect of dietary salinomycin supplementation on insulin secretory response to feeding in fattening steers. Jpn. J. Zootech. Sci. 61:271-276.
  14. Van der Walt, J. G. 1978. Volatile fatty acid metabolism in sheep. 3. Diurnal variation in the contribution of ruminal propionic acid production to the whole body glucose turnover of Merino sheep fed lucerne hay twice daily. Onderstepoort J. Vet. Res. 45:125-132.
  15. Bagley, C. P., J. I. Feazel, D. G. Morrison and D. M. Lucas. 1988. Effects of salinomycin on ruminal characteristics and performance of grazing beef steers. J. Anim. Sci. 66:792-797. https://doi.org/10.2527/jas1988.663792x
  16. Huggett, A. G. and D. A. Nixon. 1957. Enzymic determination of blood glucose. Biochem. J. 66:12P.
  17. Ambo, K., K. Yoshida and Y. Nakashima. 1989. Effect of salinomycin supplementation on plasma glucose and insulin responses to intravenous injection of glucose in sheep. Asian-Aust. J. Anim. Sci. 2:222-223. https://doi.org/10.5713/ajas.1989.222
  18. Rogers, J. A. and C. L. Davis. 1982. Rumen volatile fatty acid production and nutrient utilization in steers fed a diet supplemented with sodium bicarbonate and monensin. J. Dairy Sci. 65:944-952. https://doi.org/10.3168/jds.S0022-0302(82)82295-9
  19. Armentano, L. E., S. E. Mills, G. de Boer and J. W. Young. 1984. Effects of feeding frequency on glucose concentration, glucose turnover, and insulin concentration in steers. J. Dairy Sci. 67:1445-1451. https://doi.org/10.3168/jds.S0022-0302(84)81460-5
  20. Sano, H., Y. Terashima and T. Senshu. 1989. Insulin secretory response to feeding in sheep fed a diet supplemented with calcium, potassium and sodium propionate. Jpn. J. Zootech. Sci. 60:70-77.
  21. Arieli, A., J. E. Vallimont, Y. Aharoni and G. A. Varga. 2001. Monensin and growth hormone effects on glucose metabolism in the prepartum cow. J. Dairy Sci. 84:2770-2776. https://doi.org/10.3168/jds.S0022-0302(01)74731-5
  22. Weekes, T. E. C., Y. Sasaki and T. Tsuda. 1983. Enhanced responsiveness to insulin in sheep exposed to cold. Am. J. Physiol. 244: E335-E345.
  23. Sano, H., Y. Terashima and H. Takahashi. 1994. Effects of dietary salinomycin on postprandial changes in plasma insulin and glucagon concentrations, and insulin secretory response and action in sheep. Anim. Sci. Technol. 65:601-609.
  24. Bergman, E. N. 1990. Energy contributions of volatile fatty acids from the gastrointestinal tract in various species. Physiol. Rev. 70:567-590. https://doi.org/10.1152/physrev.1990.70.2.567
  25. Van Maanen, R. W., J. H. Herbein, A. D. McGilliard and J. W. Young. 1978. Effects of monensin on in vivo rumen propionate production and blood glucose kinetics in cattle. J. Nutr. 108:1002-1007. https://doi.org/10.1093/jn/108.6.1002