Occurrence of aflatoxin $M_1$ in milk determined by HPLC with derivatization method in Korea (1999-2000)

  • Kang, Hwan Goo (National Veterinary Research and Quarantine Service) ;
  • Cho, Joon Hyoung (National Veterinary Research and Quarantine Service)
  • Accepted : 2007.11.30
  • Published : 2007.12.30

Abstract

In this study, the levels of aflatoxin $M_1$ ($AFM_1$) in milk were determined by HPLC with derivatization method. Milk samples were purified using $C_{18}$ disposable cartridge followed by derivatization with trifluoroacetic acid and analysed using HPLC with fluorescence detection. The recoveries of AFM1 from milk samples added $AFM_1$ at a level of 0.025~0.1 ng/ml were 94.7~98.0% with detection limit of 0.009 ng/ml. The amounts of $AFM_1$ were determined below 0.05 ng/ml for all tested samples of commercial milk collected in 1999 and 2000.

References

  1. Bakirci I. A study on the occurrence of aflatoxin $M_{1}$ in milk and milk products produced in Van province of Turkey. Food Control 2001, 12, 47-51 https://doi.org/10.1016/S0956-7135(00)00020-7
  2. D'Melllo JPF, Macdonald AMC. Mycotoxins. Ani Feed Sci Technol 1997, 69, 155-166 https://doi.org/10.1016/S0377-8401(97)81630-6
  3. Dominguez L, Blanco JL, Gomez-Lucia E, Rodriguez EF, Suarez G Determination of aflatoxin $M_{1}$ in milk and milk products contaminated low levels. J Assoc Off Anal Chem 1987, 70, 470-472
  4. EL-Nezami HS, Nicoletti G, Neal GE, Donohue DC, Ahokas JT. Aflatoxin $M_{1}$ in Human breast milk samples from Victoria, Australia and Thailand. Food Chem Toxicol 1995, 33, 173-179 https://doi.org/10.1016/0278-6915(94)00130-G
  5. Faletto MB, Koser PL, Battula N, Townsend GK, Maccubbin AE, Gelbion HV, Gurtoo HL. Cytochrome P3-450 cDNA encodes aflatoxin B1-4-hydroxylase. J Biol Chem 1988, 263, 12187-12189
  6. Kim EK, Shon DH, Ryu D, Park JW, Hwang HJ, Kim YB. Occurrence of aflatoxin $M_{1}$ in Korean dairy products determined by ELISA and HPLC. Food Addit Contam 2000, 17, 59-64 https://doi.org/10.1080/026520300283595
  7. Lee HS, Cho BH, Son SW, Kang HG, Park JM. Rapid extraction and detection of aflatoxin $M_{1}$ in raw milk by high performance liquid chromatography. RDA J Veterinary Sci 1998, 40, 55-59
  8. MAFF UK. Survey of aflatoxin $M_{1}$ in retail milk and milk products. Food surveillance information sheet. 64, 1995
  9. Stubblefield RD. Optimum conditions for formation of aflatoxin $M_{1}$-trifluoroacetic acid derivative. J Assoc Off Anal Chem 1987, 70, 1047-1049
  10. van Egmond HP. Aflatoxin $M_{1}$ : occurrence, toxicity, regulation. In: Mycotoxin in Dairy Products. pp. 57-95, Elsevier, New York, 1989
  11. van Egmond HP. Monograph on residues and contaminants in milk and milk products, chapter 8 mycotoxins. In: International Dairy Federation Special Issue 9101. pp. 156-166, International Dairy Federation, Brussels, 1997
  12. van Egmond HP, WagstafTe PJ. The certification of aflatoxin $M_{1}$ in 3 milk powder samples. Report EUR 10412. Commission of the European Communities, Brussel, 1988
  13. WHO, CODEX. Report of the 32nd session of the codex committee on food additives and contaminants. 20-24, March, 2000. Codex, FAO, Rome, 2000