Successful pregnancy following surgical intrauterine insemination using frozen semen in Afghan hound dog

  • Kim, Hye Jin (College of Veterinary Medicine, Seoul National University) ;
  • Oh, Hyun Ju (College of Veterinary Medicine, Seoul National University) ;
  • Jang, Goo (College of Veterinary Medicine, Seoul National University) ;
  • Yoon, Jung Hee (College of Veterinary Medicine, Seoul National University) ;
  • Lee, Hyung Suk (Woosong Information College) ;
  • Kim, Sang Cheol (Department of Animal Science, Shingu College) ;
  • Hwang, Cheol Young (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Dae Yong (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Min Kyu (College of Veterinary Medicine, Seoul National University)
  • Accepted : 2007.02.22
  • Published : 2007.03.30

Abstract

A 3 year-old female Afghan hound came to the Veterinary Referral Hospital of the College of Veterinary Medicine, Seoul National University for artificial insemination (AI) with frozen semen. In order to inseminate, semen was frozen in USA 3 years ago. Frozen semen was sent by air from Santiago to Seoul for AI. The stud died 2 years ago, so we could only use a limited amount of frozen semen in that estrus cycle. The number of total motile spermatozoa was $59.4{\times}10^6$and the total volume was 1.2 ml. The frozen spermatozoa were thawed in $70^{\circ}C$ water for 8 sec, which were then deposited at the bilateral uterine horns by a surgical method. The number of corpus luteum was 6. Sixty days after artificial insemination resulted in the birth of 4 puppies, all of which are alive and healthy.

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