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Comparisons of Chicken Muscles between Layer and Broiler Breeds Using Proteomics

Jung, K. C.;Jung, W. Y.;Lee, Y. J.;Yu, S. L.;Choi, K. D.;Jang, B. G.;Jeon, J. T.;Lee, J. H.

  • Received : 2006.04.26
  • Accepted : 2006.11.01
  • Published : 2007.03.01

Abstract

The present study was carried out to investigate differentially expressed chicken muscle proteins using proteomics approach. More than 300 protein spots were investigated for the muscle samples in 2DE gels and the differentially expressed protein spots between pectoralis and peroneus longus muscles from Cornish and White Leghorn breeds were characterized by MALDI-TOF. In pectoralis muscles, PGAM1 protein was detected as differentially expressed between White Leghorn and Cornish breeds. On the other hand, 4 protein spots (SP22, nxf-2, SOD1, TNNI2) were differentially expressed between White Leghorn and Cornish breeds in peroneus longus muscles. These proteins assumed to be related with muscle development, growth, stress, and movements in chicken. In this experimental process, 2D reference map of the chicken muscle proteins was needed and 25 proteins, which were commonly expressed in both pectoralis and peroneus longus muscles in both breeds, were selected and characterized. Upon finishing the exact roles of the differentially expressed proteins, the identified 5 proteins will be used as valuable information for the fundamental mechanisms of muscle biology and underline genetics.

Keywords

White Leghorn;Cornish;Muscles;2-DE;MALDI-TOF MS

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Acknowledgement

Supported by : Rural Development Administration, Korea Science & Engineering Foundation