2, 4-Thiazolidindion Induced Plasticity of Myoblast (C2C12) and Satellite Cells (Porcine) - A Comparative Study

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Singh, N.K.;Chae, H.S.;Hwang, I.H.;Yoo, Y.M.;Ahn, C.N.;Lee, H.J.;Park, H.J.;Chung, H.Y.

  • 투고 : 2006.08.21
  • 심사 : 2007.02.20
  • 발행 : 2007.07.01

초록

This study was conducted to determine the difference between satellite cells (porcine) and myoblasts (C2C12) in their differentiation under the influence of 2, 4-thiazolidindion. C2C12 myoblast cells and porcine satellite cells (isolated from 10 d old $Landrace{\times}Duroc$ piglets) were grown to absolute confluency. Post confluent cells (day 0) were further exposed to adipogenic induction medium along with 2, 4-thiazolidindion ($8{\mu}M$) for 2 d. Thereafter, cells were exposed to 2, 4-thiazolidindion alone every 2 d till day 10 and analysed. The control was cultured in differentiation medium without any treatment. Increased (p<0.05) expression of transcriptional factors i.e. C/EBP-${\alpha}$ and PPAR-${\gamma}$ and transition of cells to adipocyte morphology was noticed from 2 d and 4 d onwards in satellite cells (Porcine) and myoblasts (C2C12) respectively. Myogenesis was observed to be suppressed completely in case of satellite cells compared to myoblasts in response to 2, 4-thiazolidindion. Pax-7 (transcriptional factor) appeared as a sole entity to satellite cells only, as it was not identified in case of myoblasts. Although both the cells were converting to adipoblasts, the degree of their conversion was different in response to 2, 4-thiazolidindion. Therefore, the hypothesis that satellite cells contribute various domains to the growing myoblasts appeared obscured and found to be dependent on the proliferative energy/or degree of fusion. However, it revealed satellite cells as currency to myoblasts/muscle.

키워드

Adipogenesis;Myoblast (C2C12);Satellite Cells(Porcine);Pax-7;2;4-Thiazolidindion

참고문헌

  1. Cowherd, R. M., R. E. Lyle and R. E. McGehee Jr. 1999. Molecular regulation of adipocyte differentiation. Semin Cell Dev. Biol. 10:3-10. https://doi.org/10.1006/scdb.1998.0276
  2. Doumit, M. E. and R. A. Merkel. 1992. Conditions for the isolation and culture of porcine myogenic satellite cells. Tissue and Cell 24:253-262. https://doi.org/10.1016/0040-8166(92)90098-R
  3. Green, H. and O. Kehinde. 1974. Sublines of mouse 3T3 cells that accumulate lipid. Cell 1:113-116. https://doi.org/10.1016/0092-8674(74)90126-3
  4. Hawke, T. J. and D. J. Garry. 2001. Myogenic satellite cells: physiology to molecular biology. J. Appl. Physiol. 91:534-551. https://doi.org/10.1152/jappl.2001.91.2.534
  5. Anderson, J. E. 2006. The satellite cell as a companion in skeletal muscle plasticity: currency, conveyance, clue, connector and colander. J. Exp. Biol. 209:2276-2292. https://doi.org/10.1242/jeb.02088
  6. Asakura, A., M. A. Rudnicki and M. Komaki. 2001. Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation. Differentiation 68:1432-436.
  7. Chen, J. C. and D. J. Goldhamer. 1999. Transcriptional mechanisms regulating MyoD expression in the mouse. Cell Tissue Res. 296:213-219. https://doi.org/10.1007/s004410051282
  8. Choi, S., D. J. Jung, J. J. Bong and M. Baik. 2007. Expression of B cell activating factor pathway genes in mouse mammary gland. Asian-Aust. J. Anim. Sci. 20:153-159.
  9. Katagiri, T., A. Yamaguchi, M. Komaki, E. Abe, N. Takahashi, T. Ikeda, V. Rosen, J. M. Wozney, A. Fusisawa-Sehara and T. Suda. 1994. Bone morphogenetic protein-2 converts the differentiation pathways of C2C12 myoblasts into the osteoblast lineage. J. Cell. Biol. 127:1755-1766. https://doi.org/10.1083/jcb.127.6.1755
  10. Kook, S. H., K. C. Choi, Y. O. Son, K. Y. Lee, I. H. Hwang, H. J. Lee, J. S. Chang, I. H. Choi and J. C. Lee. 2006. Satellite cells isolated from adult Hanwoo muscle can proliferate and differentiate into myoblasts and adipose-like cells. Mol. Cell. 22(2):239-245.
  11. Li, W. C., W. Y. Yu, J. M. Quinlan, Z. D. Burke and D. Tosh. 2005. The molecular basis of transdifferentiation. J. Cell. Mol. Med. 9(3):569-582. https://doi.org/10.1111/j.1582-4934.2005.tb00489.x
  12. Singh, N. K., H. S. Chae, I. H. Hwang, Y. M. Yoo, C. N. Ahn, S. H. Lee, H. J. Lee, H. J. Park and H. Y. Chung. 2006. Transdifferentiation of porcine satellite cells into adipoblasts with ciglitizone. J. Anim. Sci. doi: 10.2527/jas. 2006-524.
  13. Slack, J. M. W. and D. Tosh. 2001. Transdifferentiation and metaplasia-switching cell types. Curr. Opin. Genet. Dev. 11:581-586. https://doi.org/10.1016/S0959-437X(00)00236-7
  14. Teboul, L., D. Gaillard, L. Staccini, H. Inadera, E. Z. Amri and P. A. Grimaldi. 1995. Thiazolidinediones and Fatty Acids Convert Myogenic Cells into Adipose-like cells. J. Biol. Chem. 270:28183-28187. https://doi.org/10.1074/jbc.270.47.28183
  15. Van Barneveld, R. J. 2003. Modern pork production-Balancing efficient growth and feed conversion with product quality requirements and consumer demands. Asia Pac. J. Clin. Nutr. 12:S31.
  16. Wada, M. R., M. Inagawa-Ogashiwa, S. Shimizu, S. Yasumoto and N. Hashimoto. 2002. Generation of different fates from multipotent muscle stem cells. Devel. 129(12):2987-2995.
  17. Tontonoz, P., E. Hu, J. Devine, E. G. Beale and B. M. Spiegelman. 1995. PPAR${\gamma}$2 regulates adipose expression of the phosphoenolpyruvate carboxykinase gene. Mol. Cell. Biol. 15:351-357. https://doi.org/10.1128/MCB.15.1.351
  18. Tontonoz, P., E. Hu, R. A. Graves, A. I. Budavari and B. M. Spiegelman. 1994. mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer. Genes. Dev. 8:1224-1234. https://doi.org/10.1101/gad.8.10.1224
  19. Tosh, D. and J. M. W. Slack. 2002. How cells change theirphenotype. Nat. Rev. Mol. Cell. Biol. 3:187-194. https://doi.org/10.1038/nrm761
  20. Mc Neil, M. 2005. Adiopocyte staining with Oil-Red-O. Pennington lab, Molecular Endocrinology, Pennington Biomedical Research Centre. Baton Rouge, La.
  21. Michal, J., Z. Xiang, M. Davenport, M. Hayek, M. V. Dodson and K. M. Byrne. 2002. Isolation and characterization of canine satellite cells. In vitro Cell. Dev. Biol. Anim. 38:467-480. https://doi.org/10.1290/1071-2690(2002)038<0467:IACOCS>2.0.CO;2
  22. SAS Institute Inc. 2002. JMP, A Business Unit of SAS. Version 5, First edition, SAS Institute., SAS Campus Drive, Cary, NC 27513, USA.
  23. Schoonmaker, J. P., F. L. Fluharty and S. C. Loerch. 2004. Effect of source and amount of energy and rate of growth in the growing phase on adipocyte cellularity and lipogenic enzyme activity in the intramuscular and subcutaneous fat depots of Holstein steers. J. Anim. Sci. 82:137-148. https://doi.org/10.2527/2004.821137x
  24. Seale, P., L. A. Sabourin, A. G. Gabardo, A. Mansouri, P. Gruss and M. A. Rudnicki. 2000. Pax-7 is required for the specification of myogenic satellite cells. Cell 102:777-786. https://doi.org/10.1016/S0092-8674(00)00066-0
  25. Singh, N. K., H. S. Chae, I. H. Hwang, Y. M. Yoo, C. N. Ahn, H. J. Lee, H. J. Park and H. Y. Chung. 2007. Conversion of C2C12 myoblast into adipoblast with thiazolidinediones-A possible basis of intramuscular fat generation in meat animals. Asian-Aust. J. Anim. Sci. 20(3):432-439. https://doi.org/10.5713/ajas.2007.432
  26. Dodson, M. V. and B. A. Mathison. 1988. Comparison of ovine and rat muscle-derived satellite cells: response to insulin. Tissue and Cell 20:909-918. https://doi.org/10.1016/0040-8166(88)90032-8
  27. Jin, E. J., I. Kim, C. Y. Lee and B. C. Park. 2006. Suppressed cell proliferation and differentiation following an over-expression of myostatin is associated with inhibited expression of Insulin-like growth factor II and myogenin in rat L6 myoblasts. Asian-Aust. J. Anim. Sci. 19(10):1508-1513. https://doi.org/10.5713/ajas.2006.1508