Immunogenicity of the recombinant Pasteurella multocida toxin for development of subunit vaccine against swine atrophic rhinitis

돼지 위축성 비염 단위 백신 개발을 위한 재조합 파스튜렐라 독소 단백질의 면역원성 검정

  • Lee, Jeongmin (Institute of Life Science and Technology, Sungkyunkwan University)
  • 이정민 (성균관대학교 생명공학연구소)
  • Accepted : 2006.10.10
  • Published : 2007.03.30

Abstract

Pasteurella multocida Pasteurella multocida toxin (PMT) is a causal pathogenin atrophic rhinitis in pigs. To investigate the protective immunity and vaccination effect of recombinantPMT, the gene for PMT was isolated from the infective P. multocida D:4. The 2.3 kb XhoI/PstI fragment(PMT2.3) of PMT gene was expressed in E. coli BL21 (DE3) using the induced expression vector system.The recombinant protein of PMT2.3 having molecular weight of 84 kDa was purified by Ni-afinitycolumn chromatography. The PMT2.3 raised slightly less anti-PMT antibody titer than formalin-killedwhole cel, however, it showed more protective imunity against P. multocida D:4 infection in vaccinationand chalenge.

References

  1. Hunt ML, Adler B, Townsend KM. The molecular biology of Pasteurella multocida. Vet Microbiol 2000, 72, 3-25 https://doi.org/10.1016/S0378-1135(99)00183-2
  2. Liao CM, Huangb C, Hsuan SL, Chenb ZW, Lee WC, Liu CI, Winton JR, Chien MS. Immunogenicity and efficacy of three recombinant subunit Pasteurella multocida toxin vaccines against progressive atrophic rhinitis in pigs. Vaccine 2006, 24, 27-35 https://doi.org/10.1016/j.vaccine.2005.07.079
  3. Rhoades KR, Rimler RB. Capsular groups of Pasteurella multocida isolated from avian hosts. Avian Dis 1987, 31, 895-898 https://doi.org/10.2307/1591048
  4. Rutter JM, Rojas X. Atrohpic rhinitis in gnobiotic piglets: differences in the pathogenicity of Pasteurella multocida in combined infections with Bordetella bronchiseptica. Vet Res 1982, 110, 531-535
  5. To H, Someno S, Nagai S. Development of a genetically modified nontoxigenic Pasteurella multocida toxin as a candidate for use in vaccines against progressive atrophic rhinitis in pigs. Am J Vet Res 2005, 66, 113-120 https://doi.org/10.2460/ajvr.2005.66.113
  6. Gatto NT, Dabo SM, Hancock RE, Confer AW. Characterization of, and immune responses of mice to, the purified OmpA-equivalent outer membrane protein of Pasteurella multocida serotype A : 3 (Omp28). Vet Microbiol 2002, 87, 221-235 https://doi.org/10.1016/S0378-1135(02)00068-8
  7. Lee J, Kang S, Park SI, Woo HJ, Kwon M. Molecular cloning and characterization of the gene for outer memebrane protein H in a Pasteurella multocida (D : 4) isolate from pigs with atrophic rhinitis symptomes in Korea. J Microbiol Biotechnol 2004, 14, 1343-1349
  8. Rutter JM. Virulence of Pasteurella multocida in atrophic rhinitis of gnobiotic pigs infected with Bordetella bronchiseptica. Res Vet Sci 1983, 34, 287-295
  9. Rajeev S, Nair RV, Kania SA, Bemis DA. Expression of a truncated Pasteurella multocida toxin antigen in Bordetella bronchiseptica. Vet Microbiol 2003, 94, 313-323 https://doi.org/10.1016/S0378-1135(03)00137-8
  10. Petersen SK, Foged NT. Cloning and expression of the Pasteurella multocida toxin gene, toxA in Escherichia coli. Infect Immun 1989, 57, 3907-3913
  11. Kim H, Hwang H, Lee S, Park ES, Yoo SD, Lee J, Yang JS, Kwon M. Molecular cloning and expression of a gene for outer membrane protein H in Pasteurella multocida (A : 3): production of antisera against the OmpH. Kor J Microbiol Biotechnol 2005, 33, 274-280
  12. Nielsen JP, Foged NT, Sorensen V, Barfod K, Bording A, Petersen SK. Vaccination against progressive atrophic rhinitis with a recombinant Pasteurella multocida toxin derivative. Can J Vet Res 1991, 55, 128-138
  13. 지영철, 로승, 한정희, 한태욱. 돼지 위축성 비염 백신의 효과에 관한 연구. 대한수의학회지 2000, 40, 707-717
  14. Heddleston KH, Gallagher JE, Rebers PA. Fowl cholera: gel diffusion precipitin test for serotyping Pasteurella multocida from avian species. Avian Dis 1972, 16, 925-936 https://doi.org/10.2307/1588773
  15. Petersen SK, Foged NT, Bording A, Nielsen JP, Riemann HK, Frandsen PL. Recombinant derivatives of Pasteurella multocida toxin: candidates for a vaccine against progressive atrophic rhinitis. Infect Immun 1991, 59, 1387-1393
  16. Busch C, Orth J, Nabil D, Klaus A. Biological activity of a C-terminal fragment of Pasteurella multocida toxin. Infect Immun 2001, 69, 3628-3634 https://doi.org/10.1128/IAI.69.6.3628-3634.2001
  17. Adler B, Bulach D, Chung J, Doughty S, Hunt M, Rajakumar K, Serrano M, van Zanden A, Zhang Y, Ruffolo C. Candidate vaccine antigens and genes in Pasteurella multocida. J Biotechnol 1999, 73, 83-90 https://doi.org/10.1016/S0168-1656(99)00111-X
  18. Carter GR. Pasteurellosis: Pasteurella multocida and Pasteurella hemolytica. Adv Vet Sci Comp Med 1967, 11, 321-379