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On the Dynamic Characteristics of Cell Contact by Analyzing TIRE Images

전반사 형광 이미지 분석을 통한 세포 부착점의 운동 특성에 관한 연구

  • Published : 2007.03.01

Abstract

We carried out an image analysis of living cells forming their contacts at the bottom of the cell culturing substrate. In order to visualize the contact area selectively, we adopted total-internal-reflection-fluorescence (TIRF) method, which can illuminate the specimen volume within only several hundred nano-meters above the substrate. From the fluorescent intensity of the TRF image, we could calculate the distance of the cell surface from the substrate. As a result, we visualized the origin of cell contacts, their movements, and the change of cell-contact type from the close-contact into focal-contact with information of its vertical displacement representing the temporal evolution process of the three-dimensional cell-surface-profile near the contact area during this metamorphosis.

Keywords

Cell Adhesion;Close Contact;Evanescent Wave;Focal Contact;Metamorphosis;Total Internal Reflection Fluorescence

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