On the Dynamic Characteristics of Cell Contact by Analyzing TIRE Images

전반사 형광 이미지 분석을 통한 세포 부착점의 운동 특성에 관한 연구

  • Published : 2007.03.01


We carried out an image analysis of living cells forming their contacts at the bottom of the cell culturing substrate. In order to visualize the contact area selectively, we adopted total-internal-reflection-fluorescence (TIRF) method, which can illuminate the specimen volume within only several hundred nano-meters above the substrate. From the fluorescent intensity of the TRF image, we could calculate the distance of the cell surface from the substrate. As a result, we visualized the origin of cell contacts, their movements, and the change of cell-contact type from the close-contact into focal-contact with information of its vertical displacement representing the temporal evolution process of the three-dimensional cell-surface-profile near the contact area during this metamorphosis.


Cell Adhesion;Close Contact;Evanescent Wave;Focal Contact;Metamorphosis;Total Internal Reflection Fluorescence


  1. Cooper, J. M. and Cass, A. E. G., 2002, 'Miniaturized Screening Technologies for Drug Discovery,' Biochem. Soc. Trans., Vol. 30, part 4, pp. 802-806
  2. Newgreen, D. F., 1989, 'Physical Influences on Neural Crest Cell Migration in Avian Embryos: Contact Guidance and Spatial Restriction,' Int. J. Dev. Biol., Vol. 131, No. 1, pp. 136-148
  3. Zygourakis, K., 1996, 'Quantification and Regulation of Cell Migration,' Tissue Eng., Vol. 2, No. 1, pp. 1-26
  4. Gingell, D., Heavens, O. S. and Mellor, J. S., 1987, 'General Electromagnetic Theory of Total Internal Reflection Fluorescence: the Quantitative Basis for Mapping Cell-substratum Topography,' J. Cell Sci., Vol. 87, No. 5, pp. 677-693
  5. Burmeister, J. S., Olivier, L. A., Reichert, W. M. and Truskey, G. A., 1998, 'Application of Total Internal Reflection Fluorescence Microscopy to Study Cell Adhesion to Biomaterials,' Biomaterials, Vol. 19, pp. 307-325
  6. Axelrod, D., 1981, 'Cell-substrate Contacts Illuminated by Total Internal Reflection Fluorescence,' J. Cell Biol., Vol. 89, pp. 141-145
  7. Webb, D. J., Brown, C. M. and Horwitz, A. F., 2003, 'Illuminating Adhesion Complexes in Migrating Cells; Moving Toward a Bright Future,' Current Opinion in Cell Biol., Vol. 15, pp. 614-620
  8. Izzard, C. S. and Lochner, L. R., 1980, 'Formation of Cell-to-substrate Contacts During Fibroblast Motility: an Interference-reflexion Study,' J. Cell Sci., Vol. 42, pp. 81-116
  9. Smilenov, L. B., Mikhailov, A., Pelham Jr., R. J., Marcantonio, E. E. and Gundersen, G. G., 1999, 'Focal adhesion motility revealed in stationary fibroblasts,' Science. Vol. 286, pp.1172-1174
  10. Burridge, K. and Fath, K., 1989, 'Focal Contacts: Transmembrane Links Between the Extracellular Matrix and the Cytoskeleton,' BioEssays, Vol. 10, No. 4, pp. 104-108
  11. Toomre, D. and Manstein, D. J., 2001, 'Lighting up the Cell Surface with Evanescent Wave Microscopy,' Trends in Cell Biol., Vol. 11, No. 7, pp. 298-303

Cited by

  1. Review — Transport phenomena associated with cells incurring diseases vol.21, pp.11, 2007,