DOI QR코드

DOI QR Code

Effects of Colostrum Basic Protein from Colostrum Whey Protein: Increases in Osteoblast Proliferation and Bone Metabolism

  • Published : 2007.03.31

Abstract

Colostrum basic proteins (CBP) (MW 1$\sim$30 kDa) were isolated from bovine colostrum using a series of ultrafiltration processes and their effects on osteoblast cell proliferation and bone metabolism were investigated in cell line and animal models. Treatments with CBP (1, 10, 100 $\mu$g/mL) dose-dependently increased cell proliferation of osteoblastic MC3T3 cells. Alkaline phosphatase activity, a marker of osteoblastic phenotype, in the cells was also increased after treatments with CBP in a dose-dependent manner. Significant increases in bone density were observed in femur of ovariectomized rats which were fed a diet with 1% and 10% CBP, compared to rats fed a normal diet. These results suggest that CBP may increase bone mass and density and be useful for the prevention of bone-related diseases.

References

  1. Briese V, Straube W. 1986. Immunology of the fetomaternal interation: immunologic significance of colostrum and breas milk. Zentralbl Gynaekol 108: 73-83
  2. Davis DP. 1989. How suitable is human milk for pre-term babies? Acta Paediatr Jpn 31: 439-454 https://doi.org/10.1111/j.1442-200X.1989.tb01331.x
  3. Jayashree SM, Bhan MK, Kumar R, Bhandari N, Sazawal S. 1988. Protection against neonatal rotavirus infection by breast milk antibodies and trypsin inhibitors. J Med Virol 26: 333-338 https://doi.org/10.1002/jmv.1890260313
  4. Aynsley-Green A. 1989. New insights into the nutritional management of newborn infants derived from studies of metabolic and endocrine inter-relations during the adaptation to post-natal life. Proc Nutr Soc 48: 283-292 https://doi.org/10.1079/PNS19890040
  5. Kohno Y, Shiraki K, Mura T, Ikawa S. 1993. Iron-saturated actoferrin as a co-mitogenic substance for neonatal rat hepatocytes in primary culture. Acta Paediatr 82: 650-655 https://doi.org/10.1111/j.1651-2227.1993.tb18033.x
  6. Li SY, Geng ZC, Wang AZ, Gao YL, Li M, Wang HD, Wang WY. 1994. The relationship between PGE2 level in mothers' milk and physiological diarrhea of the baby and the treatment. Chin Med J (Engl) 107: 533-535
  7. Semchuk DM, Allen OB, O'Connor DL. 1994. Folate bioavailability from milk-containing diets is affected by altered intestinal biosynthesis of folate in rats. J Nutr 124: 1118-1125
  8. Klagsbrun M, Neumann J. 1979. The serum-free growth of balb/c 3T3 cells in medium supplemented with bovine milk. J Supramol Struct 11: 349-359 https://doi.org/10.1002/jss.400110310
  9. Steimer KS, Packard R, Holden D, Klagsbrun M. 1981. The serum-free growth of cultured cells in bovine colostrums and in milk obtained later in the lactation period. J Cell Physiol 109: 223-234 https://doi.org/10.1002/jcp.1041090205
  10. Hironaka T, Ohishi H, Masaki T. 1997. Identification and partial purification of a basic fibroblast growth factor-like growth factor derived from bovine colostrum J Dairy Sci 80: 488-495 https://doi.org/10.3168/jds.S0022-0302(97)75961-7
  11. Berseth CL, Lichtenberger LM, Morriss F Jr. 1983. Comparison of the gastrointestinal growth- promoting effects of rat colostrum and mature milk in newborn rats in vivo. Am J Clin Nutr 37: 52-60 https://doi.org/10.1093/ajcn/37.1.52
  12. Simmen FA, Cera KR, Maha DC. 1990. Stimulation by colostrum or mature milk of gastrointestinal tissue development in newborn pigs. J Ani Sci 68: 3596-3603 https://doi.org/10.2527/1990.68113596x
  13. Rosen CJ, Pollak M. 1999. Circulating IGF-I: New Perspectives for a New Century. Trends Endocrinol Metab 10: 136-141 https://doi.org/10.1016/S1043-2760(98)00126-X
  14. Beelin C, Andrew LZ. 2003. Separation of alpha-lactalbumin and beta-lactoglobulin using membrane ultrafiltration. Biotechnol Bioeng 83: 201-209 https://doi.org/10.1002/bit.10659
  15. Chung CY, Lida-Klein A, Wyatt LE, Rudkin GH, Lshida K, Yamaguchi DT, Miller TA. 1999. Serial passage of MC3T3-E1 cells alters osteoblastic function and responsiveness to transforming growth factor-beta 1 and bone morphogenetic protein-2. Biochem Biophys Res Commun 265: 246-251 https://doi.org/10.1006/bbrc.1999.1639
  16. D'Amour FF, Blood FR, Bleden DA. 1965. In: The manual for laboratory work in mammalian physiology. 3rd ed. University of Chicago Press, Chicago
  17. Kim NS, You YS, Kang CW, Choi IH. 2000. The changes of osteocalcin, bone-specific alkaline phosphatase, estrogen, IGF-I, Ca, P and bone mineral density on osteoporosis induced by ovariectomy in rats. Korea J Vet Res 40: 755-762
  18. Farquharson MJ, Speller RD, Brickley M. 1997. Measuring bone mineral density in archaeological bone using energy dispersive low angle X-ray scattering techniques. J Archaeol Sci 24: 765-772 https://doi.org/10.1006/jasc.1996.0158
  19. AOAC. 2000. Official Methods of Analysis. William H, ed. 17th ed. Association of official analytical chemists, Virginia
  20. SAS Institute 1985. SAS User's Guide: Statistics. 5th ed. SAS Institute Inc, Cary, NC, USA
  21. Amarnani S, Merriman HL, Linkhart TA, Baylink DJ, Mohan S. 1993. Autocrine regulators of MC3T3-E1 cell proliferation. J Bone Miner Res 8: 157-165
  22. Takada Y, Aoe S, Kumegawa M. 1996. Whey protein stimulated the proliferation and differentiation of osteoblastic MC3T3-E1 cells. Biochem Biophys Res Commun 223: 445-449 https://doi.org/10.1006/bbrc.1996.0913
  23. Hill CS, Wolfert RL. 1989. The preparation of monoclonal antibodies which react preferentially with human bone alkaline phosphatase and not liver alkaline phosphatase. Clin Chim Acta 186: 315-320 https://doi.org/10.1016/0009-8981(90)90050-3
  24. Stein GS, Lian JB, Owen TA. 1990. Relationship of cell growth to the regulation of tissue-specific gene expression during osteoblast differentiation. FASEB J 4: 3111-3123
  25. Parfitt AM. 1987. Bone remodeling and bone loss: understanding the pathophysiology of osteoporosis. Clin Obstet Gynecol 30: 789-811 https://doi.org/10.1097/00003081-198712000-00004
  26. Bowles SA, Kurdy N, Davis AM, France MW, Marsh DR. 1996. Serum osteocalcin, total and bone-specific alkaline phosphatase following isolated tibial shaft fracture. Ann Clin Biochem 33: 196-200 https://doi.org/10.1177/000456329603300304
  27. Aoe S, Toba Y, Yamamura J, Kawakami H, Yahiro M, Kumegawa M, Itabashi A, Takada Y. 2001. Controlled trial of the effects of milk basic protein (MBP) supplementation on bone metabolism in healthy adult women. Biosci Biotechnol Biochem 65: 913-918 https://doi.org/10.1271/bbb.65.913
  28. Epstein S. 1989. Bone derived proteins. Trends Endocri-nol Metab 1: 9-14 https://doi.org/10.1016/1043-2760(89)90023-4
  29. Carmichael RD, LoBue J, Gordon AS. 1992. Neonatal erythropoiesis. II. Bone marrow and splenic erythropoietic activity: data suggest erythropoietin transfer via maternal milk. Endocr Regul 26: 143-149
  30. Khar A. 1983. The serum-free growth of different cell types in buffalo milk plasma. Experientia (Basel) 39: 534 https://doi.org/10.1007/BF01965193
  31. Dorit N, Andrew G, Ian RR, Jillian C. 2005. Lactoferrin-A novel bone growth factor. Clin Med Res 3: 93-101 https://doi.org/10.3121/cmr.3.2.93
  32. Donovan SM, Odle J. 1994. Growth factors in milk as mediators of infant development. Annu Rev Nutr 14: 147-167 https://doi.org/10.1146/annurev.nu.14.070194.001051
  33. Bramaud C, Aimar P, Daufin G. 1997. Whey protein fractionation: isoelectric precipitation of $\alpha$-lactalbumin under gentle heat treatment. Biotechnol Bioeng 56: 391-397 https://doi.org/10.1002/(SICI)1097-0290(19971120)56:4<391::AID-BIT5>3.0.CO;2-J
  34. Kurihara N, Ikeda K, Hakeda Y, Tsunoi M, Maeda N, Kumegawa M. 1984. Effect of 1,25-dihydroxyvitamin D3 on alkaline phosphatase activity and collagen synthesis in osteoblastic cells, clone MC3T3-E1. Biochem Biophys Res Commun 119: 767-771 https://doi.org/10.1016/S0006-291X(84)80316-2
  35. Boumana AA, Scheffer PG, Ooms ME, Lips P, Netelenbos C. 1995. Two bone alkaline phophatase assays compared with osteocalcin as a marker of bone formation in healthy elderly women. Clin Chem 41: 196-199
  36. Dura RJ, Kaltsas GA, Mukherjee JJ, Plowman PN, Monson JP, Grossman AB, Besser GM. 1998. The role of cytotoxic chemotherapy in the management of aggressive and malignant pituitary tumors. J Clin Endocrinol Metab 83: 4233-4238 https://doi.org/10.1210/jc.83.12.4233
  37. Yasuhiro T, Yukihiro T, Yasuhiro M, Yishikazu M, Mutsumi M, Tomoko H, Toshiaki S, Seiichiro A, Hiroshi K, Masayoshi K, Akira T, Akira I. 2001. Milk basic protein promotes bone formation and suppresses bone resorption in healthy adult men. Biosci Biotechnol Biochem 65: 1353-1357 https://doi.org/10.1271/bbb.65.1353

Cited by

  1. Characteristics and Nutritional and Cardiovascular-Health Properties of Seaweeds vol.12, pp.2, 2009, https://doi.org/10.1089/jmf.2008.0151
  2. Modulation of immune cell proliferation and chemotaxis towards CC chemokine ligand (CCL)-21 and CXC chemokine ligand (CXCL)-12 in undenatured whey protein-treated mice vol.23, pp.12, 2012, https://doi.org/10.1016/j.jnutbio.2011.11.006
  3. Characterization of bacterial populations from Murciano-Granadina goat colostrum vol.94, pp.6, 2014, https://doi.org/10.1007/s13594-014-0179-0
  4. Effect of postpartum time and season on the physicochemical characteristics of Murciano-Granadina goat colostrum vol.68, pp.1, 2015, https://doi.org/10.1111/1471-0307.12163
  5. Supplementing formula-fed piglets with a low molecular weight fraction of bovine colostrum whey results in an improved intestinal barrier1,2 vol.92, pp.8, 2014, https://doi.org/10.2527/jas.2013-6437