Electrochemical Detection of Lead and Cadmium in Human Saliva by Anodic Stripping Voltammetry (ASV) Analysis: A Pilot Study

양극 벗김 전압전류법 (Anodic stripping voltammetry: ASV)을 이용한 인체 타액 내 납과 카드뮴의 검출: 예비 연구

  • Kim, Young-Jun (Department of Oral Medicine & Diagnosis & Research Institute of Oral Science, College of Dentistry, Kangnung National University) ;
  • Kim, Cheul (Department of Oral Medicine & Diagnosis & Research Institute of Oral Science, College of Dentistry, Kangnung National University)
  • 김영준 (강릉대학교 치과대학 구강내과.진단학 교실 및 구강과학연구소) ;
  • 김철 (강릉대학교 치과대학 구강내과.진단학 교실 및 구강과학연구소)
  • Published : 2007.12.30

Abstract

The aim of this study was to evaluate the differences of salivary lead (Pb) and cadmium (Cd) concentrations, using ASV analysis, after various pre-treatment procedures. 10 unstimulated whole saliva samples of non-exposed subjects to Pb and Cd were collected. Each sample was divided into 6 aliquots and centrifugation was performed in only 3 aliquots. After centrifugation, 3 different types of pre-treatment procedures were carried out. Also, these pre-treatment procedures were carried out for another 3 aliquots, without centrifugation. Pre-treated aliquots were analyzed electrochemically, by ASV. The results are as follows: 1. Mean concentration of Pb in saliva after centrifugation was significantly higher than that of non-centrifugation. 2. In the detection sensitivity of Pb in saliva, those of simple dilution technique by HCl and acid digestion technique by nitric acid were significantly higher than that of simple dilution technique by electrolyte. 3. Mean concentration of Cd in saliva after centrifugation was significantly higher than that of non-centrifugation. 4. In the detection sensitivity of Cd in saliva, those of simple dilution technique by HCl and acid digestion technique by nitric acid were higher than that of simple dilution technique by electrolyte. But, there were no significant differences between them.

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